Fig. 8

IHC staining for the assessment of vascular density (A-D; n = 10). The number of α-SMA⁺ vessels was increased in supporting hydrogels supplemented with Mel, ECs, and Mel + ECs compared to the FHT and FT groups (Black arrows; A-B). Along with these changes, the number of vWF⁺ vessels was also increased in FHT + Mel, and FHT ECs groups compared to the FT group (Black arrows; C-D). These changes did not reach statistically significant differences related to the FT and FHT groups. One-Way ANOVA with Tukey post hoc analysis. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001