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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology

Fig. 2

Western blotting reactivities and fluorescent labeling of hybridomas of the MS clones. a mAbs obtained in combination with MS screening were subjected to WB against Escherichia coli-expressed EGFP. A subset of them is shown in the figure, and the arrows indicated the EGFP signal. Some clones showed a strong single signal, while S2E8 and S2F1 did not give a signal. b Two hybridomas (S2E8 and S2F1) secreting mAb that did not give a signal in WB were labeled with EGFP and analyzed via FCM. Areas marked in gray indicate hybridomas labeled with EGFP. RFM, relative fluorescence units

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Journal of Biological Engineering

ISSN: 1754-1611