Fig. 4

Correlation between ELISA and SAST experimental values. a ELISA values of eight clones obtained by the EW method were plotted on the vertical axis, and SAST values were plotted on the horizontal axis, resulting in an approximate equation with small dispersion and a slope of approximately 1. b For the 54 clones obtained by the MW method, the variation was small, and an approximate equation with a slope of 0.7414 was obtained, indicating that the SAST experimental value was slightly higher than the ELISA experimental value. c ELISA and SAST values of 65 clones obtained by MS combination were plotted as in (a), showing that the dispersion was small. The slope was 0.5965, indicating that the SAST experimental values were higher than the ELISA experimental values. d ELISA and SAST values of 32 WB positive clones of MS were plotted, and an approximate equation was obtained. (e) ELISA and SAST values of 33 WB negative clones of MS were plotted to obtain an approximate equation. Note that the slope of the approximate equation of (d) was higher than that of (e); however, there was no significant difference between the two values. (f) Student's t-test analysis confirmed no significant difference between ELISA and SAST absorbance in EW; however, SAST experimental values were significantly higher than those of ELISA in MW and MS. ***; p < 0.01