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Table 1 Comparison of the efficiency of mAb acquisition by EW, MW and MS combinations

From: Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology

First Screening

Secondary screening

Abbreviation

Positive Clone %

(positive/analyzed)

 

ELISA

Western blotting

EW

1.8 (24/1300)

(14)

MIHS

MW

15.2 (57/375)

SAST

MS

63.0 (172/273)

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Journal of Biological Engineering

ISSN: 1754-1611