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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Experimental studies from shake flasks to 3 L stirred tank bioreactor of nutrients and oxygen supply conditions to improve the growth of the avian cell line DuckCelt®-T17

Fig. 3

Effect of the substitution of glutamine (strategy A) by glutamax (strategy B) in the culture medium for the DuckCelt®-T17 cell culture in shake flasks. Time evolution of cell growth (solid line) and viability percentage (dotted line) (a), of the concentration of metabolites involved in glutaminolysis (b) and glycolysis (c). Glutamine and glucose are represented in solid line and lactate and ammonium in dotted line. Results are presented as means ± SD (n = 6 for strategies A and B, n = 2 for strategy C (without glutamine))

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Journal of Biological Engineering

ISSN: 1754-1611