Fig. 5

Interaction of nanobodies A1 and F2 with AFB1. Molecular docking was used to predict the interaction between the key residues of nanobodies A1 and F2 with AFB1. A The structure of the template nanobody 3QXV is shown by the cartoon diagram, including CDR-H1 (green), CDR-H2 (blue), CDR-H3 (magenta), CDR-H4 (orange), and N-terminal (NT) (yellow) loops. B, C Schematic representing all interactions between individual nanobodies A1(B) and F2(C) with AFB1. The interacting residues of a nanobody with AFB1 are present in the panel. The AFB1 molecule is shown as a stick model, and its carbon atoms are in pink. The interactions between the AFB1 molecule and the nanobody residues are represented by orange and magenta dotted lines, indicating cation-pi interaction and hydrophobic interactions, respectively. D The percentages of the binding of the serially diluted A1 and F2 nanobodies to AFB1 were calculated. The dashed lines indicate the nanobody concentration at EC₅₀