Construct | Features | Application |
---|---|---|
pdCas9_silencer -gRNA | hU6 promoter- Silencer_gRNA -sgRNA scaffold-CAG promoter-dCas9-polyA | CRISPRi |
pdCas9_CAR-gRNA | hU6 promoter- CAR_gRNA -sgRNA scaffold-CAG promoter-dCas9-polyA | CRISPRi |
pdCas9-X | CAG promoter-dCas9-polyA | CRISPRi control |
pX459_14 | hU6 promoter- NRE_gRNA -sgRNA scaffold-CAG promoter-Cas9-T2A- PuroR-bGH polyA | CRISPR Excision |
pX459_15 | hU6 promoter- SDRE_gRNA -sgRNA scaffold-CAG promoter-Cas9-T2A- PuroR-bGH polyA | CRISPR Excision |
pX459_6 | hU6 promoter- Ova E2_gRNA -sgRNA scaffold-CAG promoter-Cas9-T2A- PuroR-bGH polyA | CRISPR HDR |
pHD_4520 | Exon 2 LHA-IRES-DsRed2-HSV TK polyA-CMV promoter –PuroR-IRES2-EGFP-SV40 polyA-Exon 2 RHA | CRISPR HDR |