Fig. 7
From: Cancer-on-chip: a 3D model for the study of the tumor microenvironment
Examples of liver cancer-on-chips. a Schematic design of the microfluidic device. (i) The left channel was used to generate the hypoxic gradient (red fluorescent image), flanked by the culture compartment composed of three adjacent channels. (ii) Image of the co-culture compartments. Reproduced from [182] with permission from the Chinese Journal of Analytical Chemistry. b Cholangiocarcinoma-on-chip to detect the CTCs in the human bile. (i) Image of the chip with its compartments. Upper module: A, sample loading chamber; B, membrane-type micromixers/micropumps; P, PBS chamber; W, waste outlet. Lower module: C, membrane-type micromixers/micropumps; D, paraformaldehyde chamber; E, Triton X-100 chamber; F - G, first and secondary antibody chambers, respectively; H, DAPI/Hoechst stain chamber; P, PBS chamber; W, waste outlet. (ii) Schematic representation of the cell capture, washing, collection, and immunofluorescence (IF) staining and analysis on-chip. Reproduced from [183] Copyright Hung et al., 2017