Fig. 9
A The effect of free PX-12, free YC-1, YC/PX, NIO/YC-PX, and blank niosome on the cell cycle of the HT-29 cell line. B HIF-1α gene expression in HT-29 cells after treatment with PX-12, YC-1, YC/PX, NIO/YC-PX, and blank niosome at their IC50 values. C The cellular protein levels of HIF-1α were checked after treatments of HT-29 cells with YC-1, PX-12, YC/PX, and NIO/YC-PX using western blotting (up). The bands were quantified by Image J (down). All tests were repeated three times and the data are shown based on (mean ± SD). *p < 0.05, **p < 0.01, and ***p < 0.001