Fig. 8
End-point viability assessment by LDH cryosection stain after pulsatile dynamic vs. static AV tissue culture: A LDH-viability stain performed on pAV tissues at the beginning of the experiment and following 14 days of incubation under high flow dynamic, low flow dynamic, static and death tissue conditions. (Left to right, representative samples shown, n = 6, scale bar: 500 µm); DAPI nuclear counterstain is visualized in the lower part of the tissue section; B Positively stained areas were quantified and relativized against cross section surface; C Staining intensity variation was metrically assessed by applying a colour threshold (two-way-ANOVA; * p < 0.05; # p < 0.1)