Fig. 3
From: The lyophilized chloroplasts store synthetic DARPin G3 as bioactive encapsulated organelles
A SDS-PAGE analysis of chloroplast-made DARPin G3 purified from fresh leaves (F.L), lyophilized leaves (L.L), fresh chloroplasts (F.Ch) and lyophilized chloroplasts (L.Ch) of transplastomic tobacco plants. TSP extracted from a mature leaf of wild-type (WT) was also loaded as a control. On the margins, the positions of molecular weight markers (Broad Range Protein Molecular Weight Markers), DARPin G3, and RbcL are indicated. B HER2-ECD binding activity and (C) affinity of chloroplast-made DARPin G3 purified from fresh and lyophilized leaves and chloroplasts of transplastomic tobacco plants. The total soluble protein of the wild-type (WT) tobacco plant and the bovine serum albumin (BSA, 1%, w/v) were used as negative controls. Data are the means ± SD of three independent experiments. Error bars represent the standard deviation of the mean