Fig. 2

Setting up the decellularization protocol for human cortical ovarian tissues from Transsexual and Chemo-POF patients. A H&E and B DAPI staining on the native tissue (N-Ovary) and present both panels (A and B) from top to bottom. C H&E and D DAPI staining on the decellularized ovarian tissues (D-Ovary) after treatment with 0.5 M NaOH and subsequent DNase for 24 h. After 24 h of decellularization, nuclear remnants left, only the collagen fibers were present. E Relative DNA content in the native cortical (N = 9) tissues compared to decellularized corresponding tissues treated for 24 h. Asterisk: significant difference between the two groups (P < 0.05). Scale bars: A, B & D = 100 μm and C = 16 μm