Fig. 6

Seeding of DCT (Decellularized Cortical Tissue) with HOCCs (Human Ovarian Cortical Cells) in vitro. Seeding of DCT using two methods: A & B injection of HOCCs and C a spinner flask. D The morphology of pre-seeding DCT, E The morphology and F H&E staining of post-seeding DCT after 7 days of co-culture with HOCCs in spinner flask. G Stereology to count the cell density and zone division in DCT, including the exterior outer surfaces (top), the center of the section, and the bottom zone. H Cell viability using the MTT assay at 0, 24, 48 and 72 h after culturing the DCT. Asterisk: significant difference between the groups (P < 0.05). Values are given as mean ± SEM, Scale bar: 10 μm