Fig. 10

After 8 weeks of surgery, the gross examinations were inspected by optical photos, and the biological and chemical composition of bilayer osteochondral grafts were assessed. The glycosaminoglycan (GAG) distribution analysis using Safranin-O staining (a). The HE stating is hematoxylin staining the nuclei in blue-purple, while eosin staining stains the cytoplasm and extracellular matrix pink (b). Red dashed line box: the chondral layer defect area; Black dashed line box: the subchondral layer defect area; Blue delta showed osteocyte