Fig. 1
From: An ovalbumin fusion strategy to increase recombinant protein secretion in chicken eggs
The generation of OVAL-E3-EGFP chickens. A Ovalbumin-(EAAAK)3-EGFP protein 3D structure predict by alphafold. B strategy of site-specific gene integration in ovalbumin locus. C schedule for generating the OVAL-E3-EGFP off-spring. D OVAL-E3-EGFP PGCs cell line established by FACs. E The sperms fluorescence was presenting in the sperms of OVAL-E3-EGFP recipients. F EGFP fluorescence was detected in the sperms of CAG-EGFP recipients but not in wild-type group. G OVAL-E3-EGFP offspring exhibited a distinct and robust mCherry signal in their comb, beak, skin, and claw. H Sanger sequencing reveals the precise integration of linker-EGFP sequence in ovalbumin locus