Fig. 2From: Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblastsImage analysis of intracellular Ca2+: Mean fluorescence intensity (MFI) of MG-63 osteoblasts stained with Ca2+ sensitive dye Fluo-3. Cell shape was determined using light microscopy images from LSM. Then, MFI of Fluo-3 was measured over the whole cell area. (LSM 780, Carl Zeiss, scale bars 20 μm)Back to article page