Fig. 3

A-C Spheroid formation using the PDMS microwell platform and (D) analysis of spheroid diameters at different initial seeding densities during 3 days of cultivation (n = 60). E Actin-normalized mRNA expression levels of osteoprogenitor, preosteoblast, and osteoblast markers in the monolayer and spheroids were measured by qPCR. n ≥ 3, * p < 0.05, ** p = 0.0019, *** p = 0.0008, **** p < 0.0001. Scale bar = 100 μm, microwell spheroids were captured under 40x magnification