Fig. 4

Comparison of microfluidic cultivation platforms after 4 days of cultivation. Spheroids cultivated in the microwell (A, B) platform in the cultivation chamber were smaller than spheroids cultivated in the open microwell platform (G). Analysis of mineralization (C, D) and viability (C´, D´) of MCT3T3-E1 cells in micropillar microfluidics, which was not sufficient for spheroid formation. (E, F) Spheroid formation in the microwell-pillar platform. The spheroids cultivated inside the microwell-pillar platform exhibited an altered decrease in sphere size (G), and propidium iodide staining showed the positive effect of dynamic cultivation on spheroids (H). I Summarizing scheme. G n = 120, *** p<0005, **** p<00001. (H) n = 30, **** p<00001. Scale bar = 160 μm