Fig. 1
From: Optimization of a recombinant BlaR-CTD protein formulation using the response surface methodology
A Agarose gel analysis of BlaR-CTD PCR product. Lane 1&5, pcr negative control; M, 500bp DNA ladder; Lane 2, 3, 4, 6, 7, and 8 show PCR product (~ 800 bp) in different concentration. Lane 8 was used for cloning. B Agarose gel analysis of pColdII vector PCR with specific primers before insertion. M, 500 bp DNA ladder; Lane 1, pColdI NdeI/HindIII PCR product; lane 2, pColdIV NdeI/HindIII PCR product; and lane 3, pET21a NdeI/HindIII PCR product. There is not any fragment with ~ 800bp length in PCR product. C Agarose gel analysis of Colony-PCR with pCold primers. Lane 4&7 are positive colonies for tranformation and other lane are negative colonies. Arrow indicates insert fragment. M, 500 bp DNA ladder. D Agarose gel analysis for evaluation of cloning. M, 500 bp marker; lane 1 & 2, double digested pColdII/BlaR-CTD construct, and lane 3, negative control (pColdII vector without insert fragment). Arrow indicates the insert fragment after double digestion