Optimization of a recombinant BlaR-CTD protein formulation using the response surface methodology

Table 3 UV Detection at 350 nm and the ratio of 280/260 nm of control and BlaR-CTD formulated with different excipients

StdOrder	RunOrder	PtType	Blocks	PH	Sucrose (mM)	Glycerol (%)	350 nm			280/260 nm
StdOrder	RunOrder	PtType	Blocks	PH	Sucrose (mM)	Glycerol (%)	T0	T1	T2	T0	T1	T2
12	1	-1	1	9	100	20	0.047	0.049	0.057	0.912	0.895	0.924
10	2	-1	1	9	300	20	0.057	0.051	0.0525	0.995	0.896	1.012
5	3	1	1	7	100	10	0.047	0.047	0.0485	0.921	0.903	0.907
1	4	1	1	7	300	10	0.051	0.048	0.048	0.922	0.911	0.922
20	5	0	1	7	300	20	0.048	0.050	0.05	0.886	0.905	0.900
13	6	-1	1	9	200	15	0.066	0.066	0.0685	0.971	0.925	0.917
16	7	0	1	6	200	15	0.050	0.046	0.0475	0.921	0.894	0.889
6	8	1	1	9	300	10	0.050	0.051	0.053	0.908	0.913	0.974
14	9	-1	1	8	200	15	0.061	0.066	0.0625	0.906	0.919	0.908
9	10	-1	1	7	100	20	0.044	0.046	0.047	0.883	0.888	0.879
4	11	1	1	8	200	15	0.046	0.047	0.0475	0.892	0.894	0.894
2	12	1	1	8	200	15	0.073	0.069	0.0715	0.902	0.937	0.949
11	13	-1	1	8	200	20	0.045	0.045	0.047	0.899	0.906	0.907
15	14	0	1	8	200	15	0.045	0.045	0.05	0.900	0.874	0.935
3	15	1	1	8	100	15	0.078	0.078	0.07	0.926	0.955	0.914
18	16	0	1	8	200	15	0.056	0.048	0.0545	0.911	0.883	1.049
17	17	0	1	8	300	15	0.053	0.052	0.0525	0.910	0.928	0.933
8	18	1	1	8	200	20	0.061	0.058	0.0635	0.807	0.861	0.906
19	19	0	1	9	100	10	0.054	0.055	0.0555	0.957	0.901	0.907
7	20	1	1	8	300	10	0.057	0.057	0.052	0.917	0.912	0.946
21	21	1	1	7	0	0	0.075	0.064	0.0555	0.950	0.870	0.926

ISSN: 1754-1611