Table 4 Analysis of variance based on CCD
From: Optimization of a recombinant BlaR-CTD protein formulation using the response surface methodology
Source | 350 nm | 260/280 nm | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
DF | Adj SS | Adj MS | F-Value | P-Value | DF | Adj SS | Adj MS | F-Value | P-Value | |
Model | 9 | 0.000078 | 0.000009 | 3.06 | 0.048 | 9 | 0.187504 | 0.020834 | 1.96 | 0.154 |
Linear | 3 | 0.000038 | 0.000013 | 4.44 | 0.031 | 3 | 0.089839 | 0.029946 | 2.82 | 0.093 |
pH | 1 | 0.000003 | 0.000003 | 1.08 | 0.322 | 1 | 0.089545 | 0.089545 | 8.43 | 0.016* |
Sucrose | 1 | 0.000034 | 0.000034 | 11.89 | 0.006* | 1 | 0.000035 | 0.000035 | 0.00 | 0.956 |
Glycerol | 1 | 0.000001 | 0.000001 | 0.35 | 0.567 | 1 | 0.000260 | 0.000260 | 0.02 | 0.879 |
Square | 3 | 0.000024 | 0.000008 | 2.77 | 0.097 | 3 | 0.095751 | 0.031917 | 3.00 | 0.081 |
pH*pH | 1 | 0.000002 | 0.000002 | 0.80 | 0.392 | 1 | 0.065304 | 0.065304 | 6.15 | 0.033* |
Sucrose*Sucrose | 1 | 0.000020 | 0.000020 | 7.23 | 0.023* | 1 | 0.013046 | 0.013046 | 1.23 | 0.294 |
Glycerol*Glycerol | 1 | 0.000001 | 0.000001 | 0.25 | 0.628 | 1 | 0.008111 | 0.008111 | 0.76 | 0.403 |
2-Way Interaction | 3 | 0.000017 | 0.000006 | 1.97 | 0.183 | 3 | 0.001913 | 0.000638 | 0.06 | 0.980 |
pH*Sucrose | 1 | 0.000010 | 0.000010 | 3.57 | 0.088 | 1 | 0.000190 | 0.000190 | 0.02 | 0.896 |
pH*Glycerol | 1 | 0.000006 | 0.000006 | 2.16 | 0.172 | 1 | 0.000903 | 0.000903 | 0.09 | 0.777 |
Sucrose*Glycerol | 1 | 0.000000 | 0.000000 | 0.18 | 0.683 | 1 | 0.000820 | 0.000820 | 0.08 | 0.787 |
Error | 10 | 0.000028 | 10 | 0.106235 | 0.010624 | |||||
Lack-of-Fit | 5 | 0.000017 | 0.000003 | 5 | 0.095928 | 0.019186 | 9.31 | 0.014 | ||
Pure Error | 5 | 0.000012 | 0.000002 | 1.40 | 0.362 | 5 | 0.010307 | 0.002061 | ||
Total | 19 | 0.000106 | 19 | 0.293739 | ||||||