Fig. 4
From: Stable, neuron-specific gene expression in the mouse brain
Step-by-step depiction of mouse perfusion, brain and spinal cord extraction and tissue processing. A The perfusion apparatus used to perfuse the mouse. (A-i) The perfusion is performed intracardially with ice-cold PBS-Heparin at a constant speed of ~ 200 µl/second. (A-ii and iii) Spinal cord extraction. B The brain is cut into two hemispheres. Each hemisphere follows different processing according to the analysis to be performed: one hemisphere is transferred from PFA/PBS 4% to sucrose 10% and 30% (B-i to iii), with every step taking 24 h. The tissue is then embedded in optimal cutting temperature compound (OCT compound) and stored at –80 °C (B-iv); the other hemisphere is transferred to TRIzol and homogenized for mRNA and protein isolation (B-v to vii)