Fig. 2

Evaluation of the SrC switch to control luciferase expression. A Structure of SeVdp vectors containing various degrons. Nluc: NanoLuc-PEST, Fluc: firefly luciferase (Luc2CP), OsTIR1: Oryza sativa F-box transport inhibitor response 1 mutant [OsTIR1(F74G)], LID: ligand-induced degron, mAID: mini auxin-inducible degron. B Inhibition of luciferase expression by Shield1-responsive Csy4. SeV(Csy4/RS-Nluc)-infected NIH3T3 cells were treated with 30 or 300 nM Shield1 for indicated time, and then Nluc and Fluc activities were determined, followed by calculating Nluc/Fluc values. Data are represented as the means ± SD of three independent experiments. ***p < 0.001. C Comparison of different degron systems. SeV(Csy4/RS-Nluc)- and SeV(Nluc-LID)-infected NIH3T3 cells were treated with Shield1 at indicated concentrations. SeV(OsTIR/AID-Nluc)-infected NIH3T3 cells were treated with 5-Ph-IAA at indicated concentrations. Twenty-four hours after addition of drugs, Nluc and Fluc activities were determined, followed by calculating Nluc/Fluc values. Data are represented as the means ± SD of three independent experiments. **p < 0.01, ***p < 0.001