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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors

Fig. 3

Reversible control of transgene expression by Shield1-responsive Csy4. A Outline of reversible control of EGFP expression in the SrC switch system. B Images of fluorescent protein expression with altered Shield1 concentration. The concentration of Shield1 was altered as indicated in the scheme to control transgene expression in NIH3T3 cells harboring SeV(Csy4/RS-EGFP). Scale bars, 100 μm. C Expression of luciferase gene with altered Shield1 concentration. Addition and removal of Shield1 (± Shield1) or DMSO (control) were repeated as indicated periods in SeV(Csy4/RS-Nluc)-infected NIH3T3 cells. The Nluc and Fluc activities were determined, and Nluc/Fluc ratio in non-treated cells (Day 0) was set to 1.0. Data are represented as the means ± SD of three independent experiments

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Journal of Biological Engineering

ISSN: 1754-1611