Fig. 4

Reduction of transgene expression by DD-Cys4 targeting to the SeV L gene. A Structure of the SeV(Csy4/RS-L) vector. B Images of fluorescent protein expression from SeVdp vectors whose replication and transcription were suppressed by Shield1-responsive Csy4. NIH3T3 cells were infected with SeV(Csy4/RS-L) or SeV(Csy4/RS-EGFP). After G418 selection, the cells were cultured with or without 300 nM Shield1 for 4 or 8 days. Scale bars, 100 μm. C Outline of transcription and replication control in the SrC switch system