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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSox

Fig. 1

Process of differentiation from human induced pluripotent stem cells (hiPSCs) into retinal pigment epithelial (RPE) cells. A Diagram of differentiation from hiPSCs into RPE cells. B Morphological observation of hiPSCs. Scale bar 250 μm. C Morphological observation of RPE cells. Scale bar 25 μm. D Quantitative real-time polymerase chain reaction (RT-qPCR) results of mRNA expression for Nanog, OCT4, SOX2, CRALBP, PMEL. E Immunofluorescence staining of ZO1, MITF, BEST, Melanoma in RPE cells. Scale bar 25 μm. Mean ± SD (**p < 0.01; ****p < 0.0001; n = 3)

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Journal of Biological Engineering

ISSN: 1754-1611