Fig. 3

Characterization of RPE-related gene and protein expression in the control group, Y27632 group, Repsox group, and Y-YR group (treated with Y27632 and Y27632 + Repsox sequentially). A Diagram of Y27632 and Repsox treatment in the Y-YR group. B Immunofluorescence staining of ZO1, NCAD in four groups. Scale bar 25 μm. C, D Western blot images and analysis of ECAD, NCAD, and Vimentin expression in four groups. E RT-qPCR results of mRNA expression for CRALBP, ECAD, NCAD, ZEB1, FN1,and ACTA in four groups. Mean ± SD (*p < 0.05; **p < 0.01; ***p < 0.001; n = 3)