Fig. 4

Morphology and gene expression of RPE spheroids in the control group, Y27632 group and Y-YR group (treated with Y27632 and Y27632 + Repsox sequentially). A, B Live and dead assay of RPE spheroids in three groups. Scale bar 250 μm. C, D Analysis and bright-field images of pigment area in RPE spheroids. Scale bar 250 μm. E Immunofluorescence staining of ZO1, Vimentin in three groups. Scale bar 25 μm. F RT-qPCR results of mRNA expression for CRALBP, ECAD, NCAD, TJP1, FN1, ACTA in three groups. Mean ± SD (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; n = 3)