Fig. 8

Engineered RPE patches on the collagen vitrigel (CV) membrane. A Scanning electron microscopy (SEM) of CV membrane and the RPE cells on CV membrane. Scale bar 1 μm. Scale bar 2 μm. B Immunofluorescence staining of ZO1 and Melanoma in the normal control (NC) group and CV group. Scale bar 25 μm. C Western blotting analysis of CRALBP and Melanoma expression in the two groups. D RT-qPCR results of mRNA expression for RPE65, CRALBP, PMEL, BEST, TJP1, GULP1, PEDF in the two groups. E The photograph of the bio-printed RPE spheroids. F The regular ZO1 expression of RPE in the circular region adjacent to spheroids. G The bright-filed image of the circular region adjacent to spheroids. H The collected RPE patches cutting from the engineered RPE sheets. Mean ± SD (*p < 0.05; **p < 0.01; ****p < 0.0001; n = 3)