Fig. 2
From: Advanced biomanufacturing and evaluation of adeno-associated virus
Development of 3-day suspensive AAV production in small-scale shaker flask. A Evaluation of transfection VCD of 2.0, 3.0, and 4.0 × 106 cells/mL. B Evaluation of ratio of plasmid DNA and VPC cells including 0.4, 0.5 and 0.6 μg/1 × 106 cells. C Viable cell density and viability of VPC pre- and post-transfection with maximal VCD of 4.53 × 106 cells/mL and harvest viability of 71% with optimal transfection conditions. D Volumetric productivity of AAV with final titer of 3.13 × 10.10 vg/mL with optimal transfection conditions. VPC cells were cultivated in 30-mL viral production medium supplemented with 6 g/L of glucose and 4 mM of GlutaMax at 37 °C, 8% CO2, and 130 rpm. The production process could be applied to four serotypes (AAV2, 5, DJ, and DJ/8)