Fig. 4
From: Advanced biomanufacturing and evaluation of adeno-associated virus
Scaled up AAV production in stirred-tank bioreactor. A VPC cell growth profile with peak VCD of 6.15 × 106 cells/mL (AAV-DJ8) or 7.60 × 106 cells/mL (AAV-DJ). VPC had better and healthier cell growth in bioreactor. B AAV concentration reached 7.17 × 1010 vg/mL (AAV-DJ8) or 8.14 × 1010 vg/mL (AAV-DJ). The 1.2–2.0 L of production cultures were performed in 2.5-L bioreactor with automatically controlled process parameters of 37 °C, pH 7.0, 210 rpm and DO 40%