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Table 1 Yield of the pcDNA-CCOL2A1 vaccine following different purification procedures

From: An innovative lab-scale production for a novel therapeutic DNA vaccine candidate against rheumatoid arthritis

Purification method

Volumetric plasmid yield (mg/L)

Specific plasmid yield (mg/g)

Concentration (mg/L)

PEG/MgCl2

11.81 ± 1.03*

1.78 ± 0.30*

670.60 ± 57.42**

AEC

6.62 ± 0.79

0.83 ± 0.05

330.81 ± 39.61

Commercial kit

6.52 ± 0.15

0.77 ± 0.11

325.75 ± 7.67

  1. Data are expressed as the means ± standard deviations of three independent experiments
  2. *P < 0.05, **P < 0.001
  3. Abbreviations: AEC Anion exchange column chromatography, PEG/MgCl2 Polyethylene glycol/magnesium chloride
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Journal of Biological Engineering

ISSN: 1754-1611