Table 2 Quality analysis of the pcDNA-CCOL2A1 vaccine via different purification procedures

A₂₆₀/A₂₈₀

UV spectrophotometer

1.98 ± 0.02

2.00 ± 0

2.10 ± 0.10

 ≥ 1.75^a

1.75–1.85^b

Endotoxin (EU/mg)

LAL assay

4.25 ± 0.40

5.31 ± 0.53

4.66 ± 0.48

 ≤ 10 EU/mg^{a, b}

 ≤ 40 EU/mg ^c

E. coli protein (μg/mg)

ELISA

Undetectable

 ≤ 1 μg/mg^a

 ≤ 10 μg/mg ^b

 < 1% ^c

E. coli gDNA (μg/mg)

Q-PCR

2.09 ± 0.18**

3.45 ± 0.57*

7.49 ± 0.07

 ≤ 2 μg /mg^a

 ≤ 10 μg/mg ^b

 < 1%^c

E. coli RNA (%)

AGE

Not visible

10.11%

Not visible

Not visible^{a, b}

 < 1%^c

Supercoiled (%)

HPLC/AGE

94.98%

71.24%

93.67%

 ≥ 90%^{a, b}

 ≥ 80%^c