Table 1 Information on the primers designed for each osteogenic protein genes
Gene | Amplicon Size(bp) | Forward primer(5' → 3') | Reverse primer(5' → 3') |
|---|---|---|---|
Mo-β-actin | 120 | AGTGTGACGTTGACATCCGT | TGCTAGGAGCCAGAGCAGTA |
Mo-RUNX2 | 130 | AAATTGCAGGCTTCGTGGTT | AATGGCTGTATGGTGAGGCT |
Mo-OPN | 182 | CCTGACCCATCTCAGAAGCA | TCCACAGAATCCTCGCTCTC |
Mo-ALP | 185 | GTATGGGCGTCTCCACAGTA | CTTCACGCCACACAAGTAGG |
Mo-Col1a1 | 88 | AACTGGTACATCAGCCCGAA | TTCCGTACTCGAACGGGAAT |
Mo-OCN | 156 | AGTGTGAGCTTAACCCTGCT | GAGGATCAAGTCCCGGAGAG |