Eggshell waste bioprocessing for sustainable acid phosphatase production and minimizing environmental hazards

Table 1 Randomized PBD for evaluating factors influencing ACP production by B. sonorensis strain ACP2

Std order	Trails	Variables													ACP productivity (U L⁻¹ min⁻¹)
Std order	Trails	X₁	X₂	X₃	X₄	X₅	X₆	X₇		X₈	X₉		X₁₀	X₁₁	Actual value	Predicted value	Residual
9	1	1	1	1	-1	-1	-1	1		-1	1		1	-1	44.2039	44.90942	-0.7055
12	2	-1	-1	-1	-1	-1	-1	-1		-1	-1		-1	-1	31.6019	32.30742	-0.7055
17	3	0	0	0	0	0	0	0		0	0		0	0	39.7448	38.38223	1.3625
10	4	-1	1	1	1	-1	-1	-1		1	-1		1	1	34.4131	35.11864	-0.7055
15	5	0	0	0	0	0	0	0		0	0		0	0	39.8417	38.38223	1.4594
3	6	1	-1	1	1	-1	1	1		1	-1		-1	-1	51.4743	52.1798	-0.7055
5	7	-1	-1	1	-1	1	1	-1		1	1		1	-1	31.6019	32.30742	-0.7055
16	8	0	0	0	0	0	0	0		0	0		0	0	39.7448	38.38223	1.3625
1	9	1	1	-1	1	1	1	-1		-1	-1		1	-1	36.3519	37.05741	-0.7055
14	10	0	0	0	0	0	0	0		0	0		0	0	39.9386	38.38223	1.5564
4	11	-1	1	-1	1	1	-1	1		1	1		-1	-1	31.0203	31.72579	-0.7055
13	12	0	0	0	0	0	0	0		0	0		0	0	39.7448	38.38223	1.3625
8	13	1	1	-1	-1	-1	1	-1		1	1		-1	1	37.2244	37.92985	-0.7055
6	14	-1	-1	-1	1	-1	1	1		-1	1		1	1	30.2448	30.95029	-0.7055
2	15	-1	1	1	-1	1	1	1		-1	-1		-1	1	30.7295	31.43498	-0.7055
7	16	1	-1	-1	-1	1	-1	1		1	-1		1	1	55.8365	56.54203	-0.7055
11	17	1	-1	1	1	1	-1	-1		-1	1		-1	1	37.4182	38.12373	-0.7055
18	18	0	0	0	0	0	0	0		0	0		0	0	39.7448	38.38223	1.3625
Variable				Code		Coded and actual levels
Variable				Code		-1			0			1
Glucose				X₁		1			5.5			10
Na-glutamate				X₂		0.1			0.3			0.5
NH₄NO₃				X₃		0.1			0.3			0.5
NH₄Cl				X₄		0.1			0.3			0.5
Eggshells				X₅		0.2			1.1			2
KCl				X₆		0.1			0.3			0.5
MgSO₄.7H₂O				X₇		0.02			0.11			0.2
NaH₂PO₄				X₈		0.02			0.11			0.2
(NH₄)₂MoO₄				X₉		0.0005			0.0015			0.0025
CrCl₂.6H₂O				X₁₀		0.0005			0.0015			0.0025
FeSO₄.7H₂O				X₁₁		0.0005			0.0015			0.0025

ISSN: 1754-1611