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Table 3 Matrix designed for B. sonorensis strain ACP2 OCCD

From: Eggshell waste bioprocessing for sustainable acid phosphatase production and minimizing environmental hazards

Std order

Trials

 

Variables

ACP productivity (U L-1 min-1)

 

Type

X1

X2

X3

X4

Actual value

Predicted value

Residual

19

1

Center

0

0

0

0

190.48

190.459

0.024

18

2

Factorial

-1

-1

1

1

140.75

143.832

-3.077

21

3

Center

0

0

0

0

191.06

190.459

0.605

14

4

Factorial

-1

1

1

-1

151.80

143.396

8.409

11

5

Factorial

-1

1

1

1

150.06

148.558

1.502

28

6

Factorial

-1

-1

-1

-1

157.62

164.528

-6.906

3

7

Factorial

1

1

1

-1

162.27

160.384

1.890

32

8

Center

0

0

0

0

190.48

190.459

0.024

4

9

Factorial

1

1

-1

-1

168.96

177.082

-8.118

16

10

Axial

0

2

0

0

174.48

173.689

0.7997

24

11

Factorial

1

-1

-1

1

129.12

148.727

-19.60

6

12

Center

0

0

0

0

190.19

190.459

-0.266

12

13

Center

0

0

0

0

190.77

190.459

0.315

17

14

Axial

0

0

2

0

158.20

166.516

-8.312

30

15

Center

0

0

0

0

189.90

190.459

-0.557

25

16

Axial

0

0

0

2

153.25

148.388

4.871

34

17

Factorial

1

-1

1

1

161.69

152.387

9.306

15

18

Center

0

0

0

0

190.77

190.459

0.315

27

19

2

Center

0

0

0

0

190.19

190.459

-0.266

36

20

Factorial

-1

-1

1

-1

146.28

141.869

4.410

26

21

Factorial

1

-1

-1

-1

156.16

153.889

2.278

20

22

Axial

0

0

-2

0

204.44

188.714

15.728

35

23

Factorial

-1

-1

-1

1

151.22

149.333

1.8902

5

24

Center

0

0

0

0

190.48

190.459

0.0242

22

25

Axial

0

-2

0

0

152.38

145.771

6.6160

13

26

Axial

2

0

0

0

158.20

147.855

10.348

7

27

Factorial

1

-1

1

-1

131.44

140.391

-8.942

9

28

Axial

0

0

0

-2

150.93

148.388

2.544

23

29

Factorial

-1

1

-1

-1

163.72

169.254

-5.525

10

30

Center

0

0

0

0

189.90

190.459

-0.557

1

31

Axial

-2

0

0

0

128.54

131.472

-2.932

29

32

Center

0

0

0

0

190.48

190.459

0.024

31

33

Center

0

0

0

0

190.77

190.459

0.315

8

34

Factorial

1

1

1

1

171.29

175.579

-4.289

2

35

Factorial

-1

1

-1

1

155.00

157.258

-2.253

33

36

Factorial

1

1

-1

1

174.48

175.119

-0.630

Variables

Code

Coded and actual levels

 

-2

-1

0

1

2

 

Glucose

 

8

16

24

32

40

MgSO4.7H2O

 

0.18

0.36

0.54

0.72

0.9

NaH2PO4

 

0.15

0.45

0.75

1.05

1.35

CrCl2.6H2O

 

0.002

0.004

0.006

0.008

0.01