Fig. 2

Effects of Aqp1 expression on cell morphology and viability. a, Representative quantitative phase microscopy images of cells engineered to express Aqp1 or GFP. Scale bar is 5 μm. Fold-change in b, dry mass, c, volume, and d, sphericity of Aqp1-expressing cells relative to GFP-cells measured using quantitative phase imaging. e, Viability of Aqp1-expressing cells relative to GFP-controls measured using the MTT assay. f, ATP levels in Aqp1-expressing cells relative to GFP. g, Caspase-3/7 activation in Aqp1-cells relative to GFP. The dashed lines denote the unit fold change representing no difference between GFP and Aqp1. Error bars in the phase imaging experiments represent the s.e.m. from n ≥ 3 images comprising 10–50 single cells. Error bars in the viability and caspase assays represent the s.e.m. from n ≥ 3 biological replicates