Direct cell reprogramming, also called transdifferentiation, allows for the reprogramming of one somatic cell type directly into another, without the need to transition through an induced pluripotent state. Th...
Authors: Alexander Grath and Guohao Dai
Citation:Journal of Biological Engineering
2019
13:14
Cardiac fibrosis is the most common pathway of many cardiac diseases. To date, there has been no suitable in vitro cardiac fibrosis model that could sufficiently mimic the complex environment of the human hear...
Authors: Mi-Ok Lee, Kwang Bo Jung, Seong-Jae Jo, Sung-Ae Hyun, Kyoung-Sik Moon, Joung-Wook Seo, Sang-Heon Kim and Mi-Young Son
Citation:Journal of Biological Engineering
2019
13:15
Detecting the arbitrary movements of fast-moving insects under field conditions is notoriously difficult because existing technologies are limited by issues of size, weight, range and cost. Here, we establish ...
Authors: Sarah E. Barlow, Mark A. O’Neill and Bruce M. Pavlik
Citation:Journal of Biological Engineering
2019
13:13
Digitalization of health care practices is substantially manifesting itself as an effective tool to diagnose and rectify complex cardiovascular abnormalities. For cardiovascular abnormalities, precise non-inva...
Authors: Rajat Vashistha, Prasoon Kumar, Arun Kumar Dangi, Naveen Sharma, Deepak Chhabra and Pratyoosh Shukla
Citation:Journal of Biological Engineering
2019
13:12
Erythromycin A (Er-A) produced by the actinomycete Saccharopolyspora erythraea is an important antibiotic extensively used in human medicine. Dissecting of transcriptional regulators and their target genes associ...
Authors: Hang Wu, Zuling Chu, Wanxiang Zhang, Chi Zhang, Jingshu Ni, Heshi Fang, Yuhong Chen, Yansheng Wang, Lixin Zhang and Buchang Zhang
Citation:Journal of Biological Engineering
2019
13:11
Hydrophobins are highly surface-active proteins that have versatile potential as agents for interface engineering. Due to the large and growing number of unique hydrophobin sequences identified, there is growi...
Authors: Bryan W. Berger and Nathanael D. Sallada
Citation:Journal of Biological Engineering
2019
13:10
ADAMTS13 (A disintegrin and metalloprotease with a thrombospondin type 1 motif 13) cleaves Von Willebrand factor (VWF) to regulate its size, thereby preventing aberrant platelet aggregation and thrombus. Defic...
Authors: Shanshan Yu, Wang Liu, Jinhua Fang, Xiaozhong Shi, Jianhua Wu, Ying Fang and Jiangguo Lin
Citation:Journal of Biological Engineering
2019
13:9
Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated and expanded from many tissues, and are being investigated for use in cell therapies. Though MSC therapies have demonstrated some s...
Authors: Andrew Hamann, Albert Nguyen and Angela K. Pannier
Citation:Journal of Biological Engineering
2019
13:7
The automation of modular cloning methodologies permits the assembly of many genetic designs. Utilising characterised biological parts aids in the design and redesign of genetic pathways. The characterisation ...
Authors: Kealan Exley, Christopher Robert Reynolds, Lorna Suckling, Soo Mei Chee, Argyro Tsipa, Paul S. Freemont, David McClymont and Richard Ian Kitney
Citation:Journal of Biological Engineering
2019
13:8
Cardiovascular disease and myocardial infarction are associated with high mortality and morbidity and a more effective treatment remains a major clinical need. The intramyocardial injection of biomaterials has...
Authors: David J. Lee, Maria A. Cavasin, Adam J. Rocker, Danielle E. Soranno, Xianzhong Meng, Robin Shandas and Daewon Park
Citation:Journal of Biological Engineering
2019
13:6
The research community is intent on harnessing increasingly complex biological building blocks. At present, cells represent a highly functional component for integration into higher order systems. In this revi...
Authors: Kara A. Davis, Pei-Jung Wu, Calvin F. Cahall, Cong Li, Anuhya Gottipati and Brad J. Berron
Citation:Journal of Biological Engineering
2019
13:5
The onset of cancer metastasis is the defining event in cancer progression when the disease is considered lethal. The ability of metastatic cancer cells to stay dormant for extended time periods and reawaken a...
Authors: Shreyas S. Rao, Raghu Vamsi Kondapaneni and Akshay A. Narkhede
Citation:Journal of Biological Engineering
2019
13:3
The ovarian follicle is the structural and functional unit of the ovary, composed of the female gamete (the oocyte) and supportive somatic cells. Follicles are not only the source of a female’s germ cell suppl...
Authors: Andrea S. K. Jones and Ariella Shikanov
Citation:Journal of Biological Engineering
2019
13:2
Articular cartilage is a specialized connective tissue, predominately composed of water, collagen, and proteoglycans, that provides a smooth, lubricated surface for articulation in joints. It has long been con...
Authors: Hannah Cash and Delphine Dean
Citation:Journal of Biological Engineering
2019
13:1
Angiogenesis is touted as a fundamental procedure in the regeneration and restoration of different tissues. The induction of de novo blood vessels seems to be vital to yield a successful cell transplantation r...
Authors: Shirin Saberianpour, Morteza Heidarzadeh, Mohammad Hossein Geranmayeh, Hossein Hosseinkhani, Reza Rahbarghazi and Mohammad Nouri
Citation:Journal of Biological Engineering
2018
12:36
Metastatic recurrence is a major hurdle to overcome for successful control of cancer-associated death. Residual tumor cells in the primary site, or disseminated tumor cells in secondary sites, can lie in a dor...
Authors: Shantanu Pradhan, John L. Sperduto, Cindy J. Farino and John H. Slater
Citation:Journal of Biological Engineering
2018
12:37
Cell-based therapy by transplantation of nucleus pulposus (NP) progenitor/notochordal cells has been proposed as a promising way to halt and reverse the progression of disc degeneration. Although some studies ...
Authors: Zhuochao Liu, Zhiyong Zheng, Jin Qi, Jun Wang, Qi Zhou, Fangqiong Hu, Jing Liang, Changwei Li, Weibin Zhang and Xingkai Zhang
Citation:Journal of Biological Engineering
2018
12:35
Pectin is a rather complex and highly branched polysaccharide strengthening the plant cell wall. Thus, many different pectinases are required for an efficient microbial conversion of biomass waste streams with...
Authors: Markus Jan Müller, Sarah Stachurski, Peter Stoffels, Kerstin Schipper, Michael Feldbrügge and Jochen Büchs
Citation:Journal of Biological Engineering
2018
12:34
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) is today one of the most reliable method for gene-editing, supporting previous gene therapies technologies such as TALEN, Meganucleases a...
Authors: Alessio Biagioni, Anna Laurenzana, Francesca Margheri, Anastasia Chillà, Gabriella Fibbi and Mario so
Citation:Journal of Biological Engineering
2018
12:33
The lymphatic system is a major circulatory system within the body, responsible for the transport of interstitial fluid, waste products, immune cells, and proteins. Compared to other physiological systems, the...
Authors: Laura Alderfer, Alicia Wei and Donny Hanjaya-Putra
Citation:Journal of Biological Engineering
2018
12:32
Biological systems have complicated environmental conditions that vary both spatially and temporally. It becomes necessary to impose time-varying soluble factor concentrations to study such systems, including ...
Authors: Joshua Garrison, Zidong Li, Barath Palanisamy, Ling Wang and Erkin Seker
Citation:Journal of Biological Engineering
2018
12:31
The retinal pigment epithelium (RPE) is essential to the health of the retina and the proper functioning of the photoreceptors. The RPE is rich in melanosomes, which contain the pigment melanin. Changes in RPE...
Authors: Maryse Lapierre-Landry, Joseph Carroll and Melissa C. Skala
Citation:Journal of Biological Engineering
2018
12:29
Silkworm genetic engineering is widely used in gene function, silk engineering and disease-resistant engineering in most of Asia. Some of the earliest promoter elements are used to control the development of s...
Authors: Zhan-Qi Dong, Zhi-Gang Hu, Hai-Qing Li, Ya-Ming Jiang, Ming-Ya Cao, Peng Chen, Cheng Lu and Min-Hui Pan
Citation:Journal of Biological Engineering
2018
12:30
Cell-based therapy has expanded its influence in cancer immunotherapy, regenerative medicine, and tissue engineering. Due to their secretory functions, differentiation capabilities, specific homing effects thr...
Authors: Daniel Y. Lee, Byung-Hyun Cha, Minjin Jung, Angela S. Kim, David A. Bull and Young-Wook Won
Citation:Journal of Biological Engineering
2018
12:28
Standard microorganism isolating technology applied for complex multiphase environmental samples such as soil or sediment needs pre-treatment steps to remove living cells from their mixed-phase microniche, by ...
Authors: M. V. Gorlenko, E. A. Chutko, E. S. Churbanova, N. V. Minaev, K. I. Kachesov, L. V. Lysak, S. A. Evlashin, V. S. Cheptsov, A. O. Rybaltovskiy, V. I. Yusupov, V. S. Zhigarkov, G. A. Davydova, B. N. Chichkov and V. N. Bagratashvili
Citation:Journal of Biological Engineering
2018
12:27
In vitro expansion of mesenchymal stem cells (MSCs) has been implicated in loss of multipotency, leading to impaired chondrogenic potential and an eventual therapeutic effect, as reported in our previous study...
Authors: Chong Shen, Tongmeng Jiang, Bo Zhu, Yiguan Le, Jianwei Liu, Zainen Qin, Haimin Chen, Gang Zhong, Li Zheng, Jinmin Zhao and Xingdong Zhang
Citation:Journal of Biological Engineering
2018
12:26
Oleaginous fungi are efficient tools to convert agricultural waste streams into valuable components. The filamentous fungus Mucor circinelloides was cultivated in whey permeate, a byproduct from cheese production...
Authors: Lauryn G. Chan, Joshua L. Cohen, Gulustan Ozturk, Marie Hennebelle, Ameer Y. Taha and Juliana Maria L. N. de Moura Bell
Citation:Journal of Biological Engineering
2018
12:25
The seeding of scaffolds with the stromal vascular fraction (SVF) of adipose tissue is a common prevascularization strategy in tissue engineering. Alternatively, adipose tissue-derived microvascular fragments ...
Authors: Thomas Später, Florian S. Frueh, Ruth M. Nickels, Michael D. Menger and Matthias W. Laschke
Citation:Journal of Biological Engineering
2018
12:24
A primary objective of synthetic biology is the construction of genetic circuits with behaviors that can be predicted based on the properties of the constituent genetic parts from which they are built. However...
Authors: Kalen P. Clifton, Ethan M. Jones, Sudip Paudel, John P. Marken, Callan E. Monette, Andrew D. Halleran, Lidia Epp and Margaret S. Saha
Citation:Journal of Biological Engineering
2018
12:23
Fermentation condition optimization and nutrients screening are of equal importance for efficient production of plasmid DNA vaccines. This directly affects the downstream purification and final quality and yie...
Authors: Juan Long, Xiao Zhao, Fei Liang, Nan Liu, Yuying Sun and Yongzhi Xi
Citation:Journal of Biological Engineering
2018
12:22
The ability to direct the cellular response by means of biomaterial surface topography is important for biomedical applications. Substrate surface topography has been shown to be an effective cue for the regul...
Authors: P. Formentín, Ú. Catalán, L. Pol, S. Fernández-Castillejo, R. Solà and L. F. Marsal
Citation:Journal of Biological Engineering
2018
12:21
Human connective tissues are complex physiological microenvironments favorable for optimal survival, function, growth, proliferation, differentiation, migration, and death of tissue cells. Mimicking native tis...
Authors: Girdhari Rijal and Weimin Li
Citation:Journal of Biological Engineering
2018
12:20
Generation of reactive oxygen species (ROS), triggered by ultraviolet radiation (UVR), is associated with carcinogenesis of the skin. UV irradiation induced superoxide anion (O2•−) is the key ROS involved in the ...
Authors: Li Xia Gao, Chunxiang Bian, Yan Wu, Muhammad Farrukh Nisar, Shida Chen, Chang Ming Li, Ling Yu, Ping Ji, Enyi Huang and Julia Li Zhong
Citation:Journal of Biological Engineering
2018
12:17
Epithelia and endothelia delineate tissue compartments and control their environments by regulating the passage of ions and solutes. This barrier function is essential for the development and maintenance of mu...
Authors: Jose Yeste, Xavi Illa, Mar Alvarez and Rosa Villa
Citation:Journal of Biological Engineering
2018
12:18
Flow cytometry, with its high throughput nature, combined with the ability to measure an increasing number of cell parameters at once can surpass the throughput of prevalent genomic and metagenomic approaches ...
Authors: Abhishek S. Dhoble, Pratik Lahiri and Kaustubh D. Bhalerao
Citation:Journal of Biological Engineering
2018
12:19
Antibiotics, which are the most important medication in human history, have brought global concerns due to their potential risk to human health and environment by accelerating the development of drug-resistant...
Authors: Sang Hoon Kim, Ee Taek Hwang and Man Bock Gu
Citation:Journal of Biological Engineering
2018
12:16
Biosafety is a key aspect in the international Genetically Engineered Machine (iGEM) competition, which offers student teams an amazing opportunity to pursue their own research projects in the field of Synthet...
Authors: Christopher M. Whitford, Saskia Dymek, Denise Kerkhoff, Camilla März, Olga Schmidt, Maximilian Edich, Julian Droste, Boas Pucker, Christian Rückert and Jörn Kalinowski
Citation:Journal of Biological Engineering
2018
12:13
Effects of shear stress on endothelium are important for the normal physiology of blood vessels and are implicated in the pathogenesis of atherosclerosis. They have been extensively studied in vitro. In one pa...
Authors: Mean Ghim, Kuin T. Pang, Mehwish Arshad, Xiaomeng Wang and Peter D. Weinberg
Citation:Journal of Biological Engineering
2018
12:15
Synthesis of a variety of biological circuits for specific functional purposes has made a tremendous progress in recent years. The ultimate goal of combining molecular biology and engineering is to realize a f...
Authors: Chun-Liang Lin, Ting-Yu Kuo and Wei-Xian Li
Citation:Journal of Biological Engineering
2018
12:14
While hypoxia has been well-studied in various tumor microenvironments, its role in cancer cell dormancy is poorly understood, in part due to a lack of well-established in vitro and in vivo models. Hypoxic con...
Authors: Hak Rae Lee, Faith Leslie and Samira M. Azarin
Citation:Journal of Biological Engineering
2018
12:12
Anti-CRISPR proteins are expressed by phages as a reaction to the bacterial CRISPR–Cas defense system. Recently, the structures of anti-CRISPR proteins have been determined, and their diverse functions have be...
Authors: Jing Li, Zengliang Xu, Aleksandr Chupalov and Mario Andrea Marchisio
Citation:Journal of Biological Engineering
2018
12:11
Petrochemical wastewaters are highly polluting due to having various destructive materials such as aromatic hydrocarbons and heavy metal ions. Therefore, they need to be treated before disposal to the environm...
Authors: Mahmood Derakhshan and Mojtaba Fazeli
Citation:Journal of Biological Engineering
2018
12:10
Regulatory genes play critical roles in natural product biosynthetic pathways. Chromomycins are promising anticancer natural products from actinomycetes. This study is aimed to create an efficient strain for p...
Authors: Lei Sun, Jia Zeng, Peiwu Cui, Wei Wang, Dayu Yu and Jixun Zhan
Citation:Journal of Biological Engineering
2018
12:9
Coral reefs are colored by eukaryotic chromoproteins (CPs) that are homologous to green fluorescent protein. CPs differ from fluorescent proteins (FPs) by intensely absorbing visible light to give strong color...
Authors: Josefine Liljeruhm, Saskia K. Funk, Sandra Tietscher, Anders D. Edlund, Sabri Jamal, Pikkei Wistrand-Yuen, Karl Dyrhage, Arvid Gynnå, Katarina Ivermark, Jessica Lövgren, Viktor Törnblom, Anders Virtanen, Erik R. Lundin, Erik Wistrand-Yuen and Anthony C. Forster
Citation:Journal of Biological Engineering
2018
12:8
Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase...
Authors: Fouzia Tabssum, Muhammad Irfan, Hafiz Abdullah Shakir and Javed Iqbal Qazi
Citation:Journal of Biological Engineering
2018
12:7
Regenerative medicine and tissue engineering are promising approaches for organ transplantation. Extracellular matrix (ECM) based scaffolds obtained through the decellularization of natural organs have become ...
Islet transplantation is increasingly used in the diabetic patients to control the blood glucose level. However, the functional output of transplanted islets remains hampered due to the local inflammation, los...
Authors: Kelei Zhu, Leqi Dong, Jinbo Wang, Dingyao Li, Mingliang Chen, Cunbin Jiang and Jinfa Wang
Citation:Journal of Biological Engineering
2018
12:5
Protecting drinking water supplies from pathogens such as Cryptosporidium parvum is a major concern for water utilities worldwide. The sensitivity and specificity of current detection methods are largely determin...
Authors: L. Diéguez, M. Winter, S. Molan, P. Monis, B. King and B. Thierry
Citation:Journal of Biological Engineering
2018
12:4
Tryptophan derivatives such as 5-hydroxytryptophan (5HTP) and serotonin are valuable molecules with pharmaceutical interest. 5HTP is presently mainly obtained by extraction from the plant Griffonia simplicifolia ...
Authors: José-Aníbal Mora-Villalobos and An-Ping Zeng
Citation:Journal of Biological Engineering
2018
12:3
As a family of hormones with pleiotropic effects, natriuretic peptide (NP) system includes atrial NP (ANP), B-type NP (BNP), C-type NP (CNP), dendroaspis NP and urodilatin, with NP receptor-A (guanylate cyclas...
Authors: Shihui Fu, Ping Ping, Fengqi Wang and Leiming Luo
Citation:Journal of Biological Engineering
2018
12:2