Table 2 P. tricornutum fragments used for assembly of chromosomes 25 and 26
Fragment # | Fragment size (kb) (final sizes with KmArs (frags 2,3,4) and Ura (frag 3)) | Restriction sites used to cut genomic DNA for TAR cloning | Release sites (5’ and 3’) | 5’ overlap (kb) | 3’ overlap (kb) |
|---|---|---|---|---|---|
25-1 | 105.5 | NotI (3’ only) | I-CeuI + I-SceI | 0.6 | 35.4 |
25-2 | 126.9 | FseI (3’ only) | I-CeuI + I-SceI | 35.4 | 9.6 |
25-3 | 122.3 | SrfI | I-CeuI + I-SceI | 9.6 | 27.8 |
25-4 | 125.2 | FseI (5’ only) | I-CeuI + I-SceI | 27.8 | 32.8 |
25-5 | 127.5 | PmeI (5’ only) | I-CeuI + I-SceI | 32.8 | 0.6 |
26-1 | 76.6 | AscI (3’ only) | I-CeuI + I-SceI | 0.5 | 26.1 |
26-2 | 142.6 | SwaI | I-CeuI + I-SceI | 26.1 | 39.5 |
26-3 | 129.3 | NotI + AscI | I-CeuI + I-SceI | 39.5 | 25.1 |
26-4 | 116.6 | AsiSI | I-CeuI + I-SceI | 25.1 | 12.1 |
26-5 | 83.1 | AbsI (5’ only) | I-CeuI + I-SceI | 12.1 | 0.5 |