- Letters to the Editor
- Open Access
An improved Escherichia colistrain to host gene regulatory networks involving both the AraC and LacI inducible transcription factors
© Kogenaru and Tans; licensee BioMed Central Ltd. 2014
- Received: 2 October 2013
- Accepted: 14 December 2013
- Published: 2 January 2014
Many of the gene regulatory networks used within the field of synthetic biology have extensively employed the AraC and LacI inducible transcription factors. However, there is no Escherichia coli strain that provides a proper background to use both transcription factors simultaneously. We have engineered an improved E. coli strain by knocking out the endogenous lacI from a strain optimal for AraC containing networks, and thoroughly characterized the strain both at molecular and functional levels. We further show that it enables the gradual and independent induction of both AraC and LacI in a simultaneous manner. This construct will be of direct use for various synthetic biology applications.
- Escherichia coli
- Gene network
- Transcription factor
- Regulatory cascade
Synthetic biology often deals with engineering of biological circuitry to obtain desired phenotypes. The successful design and construction of the first synthetic gene circuits like the genetic toggle switch  and the repressilator  have demonstrated that engineering-based methodology could indeed be used to build sophisticated, computing-like behavior into biological systems. Well-understood transcription factors are the key element for such circuits. Although many different transcription factors are found in nature, majority of them have not been well-characterized, and even their performance may be unpredictable . Further, it is becoming evident that the host strain has a profound influence on the synthetic gene circuits phenotypic response . Hence synthetic biology field has extensively used just a few well-characterized parts, in particular the arabinose operon regulator (AraC), lactose operon regulator (LacI), tetracycline operon regulator (TetR) and λ phage regulator (CI).
A plethora of E. coli strains is available to harbor synthetic regulatory networks built from TetR and CI parts, owing to their exogenous nature. However, the choice is limited for the networks that use the LacI and AraC parts, as they are endogenous to E. coli. Currently, there are ~80 strains available with a lacI mutation at the coli genetic stock center, which may in principle be used to harbor constructs with the LacI, TetR and CI. For applications that aim to combine the AraC and LacI, one may consider strains KL390 (CGSC#6207), KL384 (CGSC#6205) and KL385 (CGSC#6206), since they have mutations in both the araC and lacI. However, these strains lead to an all-or-none response by the araBAD promoter (PBAD), in which cells either display full expression or only basal expression, while gradual induction cannot be achieved, especially at the low concentration of the L-Arabinose inducer . This all-or-none phenomena occurs because, expression of the inducer transporter is controlled by the inducer itself . Additionally, these strains contain arabinose metabolizing genes, which results in the decrease of the effective concentration of L-Arabinose as the cells utilize it.
Here we constructed a host suitable for gene regulatory networks involving both the AraC and LacI transcription factors. We used the strain BW27783 (CGSC#12119) as a basis, since it carries a deletion for the arabinose metabolizing genes, and also abolished the all-or-none response, with a copy of the low-affinity high-capacity transporter, araE, under the control of an arabinose-independent promoter . To allow for networks containing LacI as well, we knocked out the endogenous lacI copy from the BW27783 strain, using lambda Red recombinase mediated site-specific genome engineering technology . We characterized the resulting new strain for the possible effects on the growth rate, and further show that it allows for the gradual and independent induction of AraC and LacI.
The parent strain BW27783 carries a ∆lacZ4787(::rrnB-3) mutation, consisting of three tandem copies of rrnB transcriptional terminators inserted within the promoter of the lactose (lac) operon . This suppresses the expression of lac operon, which was confirmed by the LacZ assay (see Additional file 1: Figure S1). Hence deletion of the lacI in this strain will not lead to the over expression of the lac operon.
In order to further confirm the lacI deletion at the level of protein, we carried out Western blot analysis on both the parent and engineered strains (Figure 1c). This confirms that the parent strain indeed expresses lacI repressor, as the protein was detected around 40 kilo Dalton (kDa). In particular, the engineered strain MK01 didn’t show any detectable protein around that size, confirming the complete knockout of the lacI repressor (Figure 1c).
To assess the possible effects on the growth, we measured the relative accumulation of the parental and engineered cells in a stationary-phase culture. For this, we labeled these cells with two different fluorescent proteins (eYFP and mCherry), using constitutively expressing constructs (see Additional file 1: Figure S2a and b), and performed competition experiment in M9 minimal medium. We initiated the competition by mixing equal densities of the cultures from both the strains and measured their relative abundance after 15 hours of the growth. This assay revealed that the engineered MK01 strain accumulated 5.6% less than the parent BW27783 strain (Figure 1d). After swapping the fluorescent protein marker, MK01 again accumulated less than the parent strain (15.8%, Figure 1e). These observations could be attributed to the constitutive expression of the cat selection cassette used to replace the lacI. Additionally, maintenance of the whole reporter plasmids used to label the strains may also influence the growth rates . Furthermore, addition of the inducers L-Arabinose and Isopropyl β-D-1-thiogalactopyranoside (IPTG) did not show any differences in the relative accumulation, consistent with the idea that these inducers are non-metabolizable (Figure 1d and e).
The strain BW27783 (genotype: F-, ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), λ-, ∆(araH-araF)570(::FRT), ∆araEp-532::FRT, φPcp8-araE535, rph-1, ∆(rhaD-rhaB)568, hsdR514)  was used as a parental strain to derive the MK01 strain by following one-step inactivation of chromosomal genes procedure . More detailed procedure on strain engineering can be found in the Additional file 1.
The expression of LacI protein was assayed by Western blotting using a mouse anti-LacI monoclonal antibody (Abcam), and a sheep anti-mouse secondary antibody (Jackson ImmunoResearch) conjugated to horseradish peroxidase.
Strains transformed with various DNA constructs (Additional file 1) were grown overnight in M9 minimal medium with appropriate antibiotics at 37°C. These cultures were further diluted to an optical density of 0.001 at 550 nm in the measuring plate. After 6 to 15 hours of further growth, cells were measured for fluorescence, using a BD LSRFortessa™ cell analyzer flow cytometer. The eYFP fluorescence was measured using a 488 nm excitation laser and a 515–545 nm emission filter, while mCherry was measured using a 561 nm excitation laser and 600–620 nm emission filter. A minimum of 10,000 cells was measured from each sample. From the single-cell fluorescence intensities, the mean fluorescence intensity per cell, representing the population average was calculated.
This work is part of the research programme of the Stichting voor Fundamenteel Onderzoek der Materie (FOM), which is financially supported by the Nederlandse Organisatie voor Wetenschappelijke Onderzoek (NWO).
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