Table 4 Bacterial strains

IJ1133

E. coli K-12 F ^-Δ lacX74 thi Δ (mcrC-mrr)102::Tn10

Host for measuring phage titers and some serial transfers to determine the evolutionary stability of the dsp B transgene in the absence of an extracellular PNAG matrix.

[31]

BL21 (DE3)

E. coli B Gal ^-dcm ompT hsdS(r _B - m _B -) [ λ(DE3)]

Host for protein expression

[32]

IJ512

E. coli K12 Δ lacX74 supE44 galK2 galT22 mcrA rfbD1 mcrB1 hsdS3 /F ^′lac

Host for selecting T7 carrying T3 gene 1.2

Δ trxA

JW5856 (Keio Δ trxA::kan)

Host for selection of positive phage recombinants carrying trxA

KEIO [33]

Δ csrA

E. coli K-12 F ^-Δ lacU169 Δ csrA::kan

Biofilm host; overproduces PNAG, the substrate for dispersin B

TR1-5 mutant in [34]

Δ nagZ

JW1083 (Keio Δ nagZ::kan)

Host with low background activity for producing dispersin B protein from phage lysates

KEIO [33]