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Table 2 ZIKV biosensors developed in 2016

From: Future developments in biosensors for field-ready Zika virus diagnostics

Assay technique Detection mode Target Sample matrix Range of detection Limit of detection Sample volume Assay time Cost per assay Ref.
Immunoassay Impedimetry NS1 (flaviviruses) PBS
Serum
10-2000 ng/mL
10-1000 ng/mL
3 ng/mL
30 ng/mL
40 μL 30 min unspecified [61]
Capacitance NS1 (flaviviruses) PBS
Serum
5-1000 ng/mL
5-1000 ng/mL
0.2 ng/mL
0.5 ng/mL
Immunoassay Chemiluminescence E protein (ZIKV) PBS
Urine, plasma
10-105 PFU/mL
10-104 PFU/mL
10 PFU/mL
10 PFU/mL
100 μL ~2 h unspecified [68]
LAMP AC susceptometry NS5 oligonucleotide (ZIKV) Unspecified
Serum
1-103 aM
1-104 aM
1 aM
1 aM
40 μL <30 min unspecified [41]
NASBA-CRISPR Colorimetry RNA (ZIKV) Serum 3 fM - 30 pM 1 fM 300 μL ~3 h $0.10-$1 [39]
RT-LAMP Colorimetry E protein RNA (ZIKV) Saliva 50-5 × 104 PFU/mL 50-100 PFU/mL 65 μL 40 min ~$2 [40]
RT-SIBA Fluorescence RNA (ZIKV) Lysis buffer 5 × 103-5 × 106 copies/mL 5000 copies/mL 2 μL <30 min unspecified [42]