Fig. 4

Opportunities for innovating global 5mC analysis methods. a Comparison of tools based on LC-MS/MS with immunoquantification to perform global methylation analysis. The red check mark indicates the method that displays superiority on the basis of the indicated criterion. b The authors’ illustration of how the current global DNA methylation derivatives’ analysis methods fare on the basis of analytical superiority (aggregate of detection limit, input genomic DNA and robustness wherever available) and feasibility of translation (aggregate of time, complexity and cost)