Skip to main content

Gentamicin-loaded chitosan/folic acid-based carbon quantum dots nanocomposite hydrogel films as potential antimicrobial wound dressing



To provide effective healing in the wound, various carbohydrate polymers are commonly utilized that are highly potent platforms as wound dressing films. In this work, novel antibacterial flexible polymeric hydrogel films were designed via crosslinking polymeric chitosan (CS) with folic acid-based carbon quantum dots (CQDs). To end this, folic acid as a bio-precursor is used to synthesize CQDs through the hydrothermal technique. The synthesized CQDs as a crosslinking agent was performed at different concentrations to construct nanocomposite hydrogel films via the casting technique. Also, gentamicin (GM), L-Arginine and glycerol were supplemented in the formulation of nanocomposite since their antibiotic, bioactivity and plasticizing ability, respectively.


The successful construction of films were verified with different methods (FT-IR, UV-Vis, PL, SEM, and AFM analyses). The GM release profile displayed a controlled release manner over 48 h with a low initial burst release in the simulated wound media (PBS, pH 7.4). Antibacterial and in vitro cytotoxicity results showed a significant activity toward different gram-positive and negative bacterial strains (about 2.5 ± 0.1 cm inhibition zones) and a desired cytocompatibility against Human skin fibroblast (HFF-1) cells (over 80% cell viability), respectively.


The obtained results recommend CQDs-crosslinked CS (CS/CQD) nanocomposite as a potent antimicrobial wound dressing.

Graphical Abstract


Skin, the widest organ of the body, does multifunctional tasks. Its injuries including all kinds of pressure sores, ulcer and burn wounds, and also other abrasive and traumatic wounds are highly costly to be cured every year. Hence, to make a good method in wound healing, lots of research on wound dressing are done [1, 2]. The infection created as a result of microorganisms such as bacteria in the injured section of the skin is one of the important challenges to wounds healing [3]. Further problems such as delay in skin recovery may occur as a result of this infection. Recently widespread range of science in different areas such as MRI contrast agents, injectable hydrogel, drug delivery, and another type of biomedical applications have concentrated on biodegradable polymers [4, 5]. For example, to provide effective healing in the wound, various biomaterials are commonly utilized [6]. Among biomaterials, carbohydrate polymers and relative hydrophilic ones are highly potent platforms as wound dressing films [7].

Chitosan, a carbohydrate polymer, has been extensively researched in different fields since its exclusive features such as biodegradability, biocompatibility, availability, etc. [8, 9]. Moreover, it can be generally employed in bio-based and biomedical applications owing to its biodegradability, biocompatibility, anti-infection, antimicrobial, and hemostatic ability properties [10,11,12]. On the other hand, most of these applications of chitosan are related to its hydrogel forms. The main required method for the preparation of hydrogels is the crosslinking types including chemical and physical [13,14,15]. While in the latter type networks are generated through intermolecular interactions, in the former, the chemical type, they are constructed via covalent bonding. CS hydrogel as a talented matrix platform is respected from a material point of view for its renewability, non-toxicity, biocompatibility, biodegradability, and supramolecular structure [16]. Particularly, the incorporation of different nanoparticles makes CS-based nanocomposites good candidate in biomedical fields that attracted a considerable attention [17]. Recently, the cytotoxicity of nanoparticles and crosslinking is of great challenge, particularly in biomedical applications. Therefore, it is essential to find convenient and secure crosslinkers with proper cytocompatibility and biodegradability.

In recent decades, there has been significant attention in using biocompatible nanomaterials as crosslinking agents for the construction of polymeric hydrogel [18,19,20,21]. Remarkably, fluorescent hydrogels received great attention in biomedical applications, which has been offered by the integration of individual chemical and physical characteristics of photoluminescence (PL) nanomaterials, for instance, semiconductor quantum dots, up-conversion nanoparticles, carbon quantum dots (CQDs), and luminescent metal complexes [22,23,24,25]. Because of great photo-physical and chemical features, CQDs have been regarded as a class of PL nanomaterial, absorbing remarkable and attracting interest in both technology and scientific fields [26, 27]. Likewise, they have been broadly employed in white light-emitting diodes, optoelectronic devices, catalysis, drug delivery, chemo/biosensors, bioimaging, etc. [28, 29]. Recently, a significant amount of research has been implemented on the synthesis of CQDs from bio-based materials, i.e., folic acid [30,31,32]. As a vital dietary component and one of the B vitamins, folic acid can well reduce the risk of some diseases [33]. It has a good potential to be adequately polymerized, condensed, and carbonized to produce N-doped CQDs without using any passivation or additive agents because of the containing rich nitrogen and functional groups of –COOH, –NH2, and –OH, in its structure [30].

As the above mentioned stated of art, we opted to construct innovative fluorescent hydrogel films as a potential wound dressing application based on chitosan as an easily obtainable carbohydrate. For this purpose, folic acid-based CQDs were selected as a green agent for the crosslinking chitosan because of its great biocompatibility, high surface functionality, and also controlling drug release ability. Hydrogel films of CQDs-crosslinked chitosan (CS/CQD) nanocomposite were prepared via a straightforward casting technique. Gentamicin (GM) is a powerful commercially available antibiotic drug prescribed commonly due to its efficacy in killing a variety of Gram-positive and negative bacteria. Also, the effect of CQDs content on the structural features and release manner of the hydrogel films was deliberate. Moreover, their antibacterial activity against different types of bacterial, in-vitro GM release, blood clotting, hemolysis, and cytotoxicity toward Human skin fibroblast HFF-1 cell lines were studied, enabling us to distinguish the ability of CS/CQD as an antibacterial film. This procedure may open a new vision in constructing a simple platform as antimicrobial wound dressing.

Results and discussion

CS/CQD preparation

In this study, hydrogel films with the nanocomposite of CQDs and chitosan biopolymer were designed (Fig. 1). First, folic acid as a bio-precursor is used to synthesize CQDs through the hydrothermal technique. Next, CS/CQD nanocomposites were prepared by incorporating GM as the antibiotic agent and different concentrations of CQDs as a crosslinking agent within the CS matrix via the straightforward casting method. L-Arg and Glycerol were also added in the nanocomposite formulation since their bioactivity and plasticizing capability, respectively. The carboxylate functional groups in the CQDs structure make them potential to interact with CS by generating electrostatic interaction and hydrogen bonding with its amine and hydroxyl functional groups, respectively [20]. The prepared CS/CQD nanocomposite hydrogel films with controlling the release profile of GM could be considered as an environmentally friendly, cytocompatible and low-cost anti-infection platform.

Fig. 1
figure 1

Schematic representation of the crosslinking CS with CQDs toward the preparation of CS/CQD nanocomposite hydrogel film

Characterization of the materials

The FT-IR spectra of CS, CS/CQD 5%, CS/CQD 10%, and CS/CQD 15% are considered to realize the successful construction of nanocomposites and also their possible interactions (Fig. 2). In the CS FT-IR spectrum, the peaks appeared at 1604 and 1590 cm− 1 are related to their C = O stretching vibrations of amide moieties and the N–H bending vibrations of amines, respectively. Comparison of the spectrum of CS and CS/CQD nanocomposite hydrogel films shows an appearance of a new peak at 1520 cm− 1 ascribed to the skeletal vibration of aromatic rings. Also, the peaks at the range of 3200–3600 cm− 1 related to the stretching vibrations of O-H become wider in the spectrum of CS/CQD that can be due to the H-bonding correlations between CS and CQDs. The appearance of these alterations can confirm the presence of CQDs in the CS polymeric network [20].

Fig. 2
figure 2

FT-IR spectra for the CS (A), CS/CQD 5% (B), CS/CQD 10% (C), and CS/CQD 15% (D)

The optical and luminescent properties of the prepared films were evaluated using UV–vis and PL spectroscopy techniques, respectively. Figure 3A displays the desperation UV–vis spectra of CQDs, CS/CQD 5%, CS/CQD 10%, and CS/CQD 15%. A characteristic π-π* transition absorption peak related to the aromatic sp2 domains, a typical n-π* transition absorption, and a long tail widening into the visible range are observed for CQDs [34]. After the incorporation of CQDs within the CS polymeric matrix, a steady blueshift was observed for the n-π* transition absorption peak in UV–vis spectra of CS/CQD nanocomposite. This is probably due to electrostatic interaction between CQDs and CS and consequently variation in the electronic transitions of π-π* and n-π* by refilling or depleting the CQDs valance bands. The maximum emission wavelength for CQDs was observed at 450 nm (Fig. 3B). Due to the high ultimate fluorescence feature of folic acid-based CQDs, it is triggered in composition with CS, which can visibly induce in the CS/CQD nanocomposites (photographic images under UV 365 nm lamp in Fig. 3B). Also, another emission wavelength can be seen in the PL spectra of CS/CQD nanocomposites at about 570 nm. For these observations, some reasons have been reported such as the self-absorbing, filter effect, inner photoluminescence reabsorption, internal or external transformations phenomena, partial quenching, extinction effect, and intersystem transitions [35]. As the exact mechanism is not completely known, we recommended further investigations to fully address this subject.

Fig. 3
figure 3

UV (A) and PL (B) spectra for the CQDs (A), CS/CQD 5% (B), CS/CQD 10% (C), and CS/CQD 15% (D) with the photographic digital image under sun and UV lamp (365 nm)

Figure 4 displays the SEM images of the CS film and CS/CQD with different concentrations of CQDs from 5 to 15%. From the SEM images, all films have a uniform surface morphology; however, by increasing the amount of CQDs in the nanocomposite, the surface morphology of the nanocomposite was slightly changed to be rough. On the other hand, there are visible the morphology of hydrogel films be a little altered by increasing CQDs (Fig. 5, AFM images). This contribution may be attributed to the nanoparticles aggregation by rising their concentration in the nanocomposite hydrogel films.

Fig. 4
figure 4

SEM images of CS film (A), CS/CQD 5% (B), CS/CQD 10% (C), and CS/CQD 15% (D) hydrogel films

Fig. 5
figure 5

AFM images of CS (A), CS/CQD 5% (B), CS/CQD 10% (C), and CS/CQD 15% (D)

The CS film and CS/CQD 5%, CS/CQD 10%, and CS/CQD 15% showed a tensile strength of 3.43, 4.26, 5.86, and 6.60 MPa, respectively (Fig. 6a). The CQDs incorporation in the CS network increased the tensile strength of films due to its crosslinking capability. Comparing the tensile strength value of CS/CQD with different CQDs concentrations showed an enhancement with rising the CQDs content. To consider the flexibility of the films, their elongation at break values was also considered. This value for CS film without CQDs is higher than that of CS/CQD nanocomposites (Fig. 6b). Whereas the integration of CQDs reduced the flexibility of the samples, they were suitable to allocate the operational force for standing over a variety of bodies or wound surfaces [36, 37].

Fig. 6
figure 6

Stress (A) and elongation at break (B) of the CS and CS/CQD with different CQDs content

Figure 7A demonstrates the swelling performance of the films in PBS (pH 7.4) at 37 °C. It was shown that the swelling ratio was noticeably raised with immersing time. The incorporating CQDs in the polymeric matrix of CS diminishes the swelling ratio. This can be due to the more crosslinking of the CS biopolymer and rigidity of the hydrogel network [21]. From the swelling results, the prepared nanocomposite hydrogel films can absorb wound moisture and release the loaded drug.

Fig. 7
figure 7

The swelling ratio in PBS (pH 7.4) at 37 °C for the CS and CS/CQD nanocomposite hydrogel films (the average of three measurements was reported) (A). GM release from the prepared CS and CS/CQD nanocomposite hydrogel films in PBS (pH 7.4) at 37 °C (B). The results of the whole blood clotting index (C) and hemolysis rate (D) of CS/CQD nanocomposite hydrogel films after incubation with human blood and human red blood cells (erythrocytes), respectively

In-vitro drug release study

Figure 7B shows the GM release chart of the CS and CS/CQD nanocomposite hydrogel films achieved in PBS (pH 7.4) at 37 °C. GM release chart exhibited a controlled and continued release over 48 h with a lower initial burst release. According to the literature [38], this can depend on the diverse interaction between CQDs and GM molecules, for example, electrostatic interaction, π-π stacking, and hydrogen bonding, which is in agreement. Therefore, the content of CQDs in the polymeric CS matrix impacts the release manner of GM. It was shown a diminish in GM release with increasing the CQDs concentration in the nanocomposites. Not only this is related to the increasing the possible interaction between CQDs and GM drug, but also the network rigidity of hydrogel affects this contribution, which is confirmed in the section of materials characterization. The results of release study are comparable with the similar reports on the GM delivery with respect to the initial burst release [39, 40]. Therefore, the prepared nanocomposite films are suitable platform to release a certain dosage of GM to the wound tissue and prevent any possible side effects of its exploded initial release.

Blood clotting and hemolysis studies

A key factor related to the wound dressing based on biomaterials is the blood clotting ability. The results of the blood clotting study are represented in Fig. 7C. According to the results, the blood capability of nanocomposite films with raising the concentration of CQDs has enhanced. The blood clotting ability of CS/CQD 15% showed a higher blood clotting ability than that of other films, as well as it is comparable with commercial Gauze. Probably, this is can be related to the bioactivity of folic acid-based CQGs. Obtained results reveal the haemostatic potential of the prepared CS/CQD nanocomposite as the wound dressing applications [41].

In the normal human blood, the erythrocyte rate is ~ 50% and its interaction with blood-contacting platforms is critical to consider the hemoglobin release. The hemolytic effects were respectively increased with hemolysis rate of 11%, 20%, and 34% with raising the concentration of CQDs in the formulation of CS/CQD nanocomposites (CS/CQD 5%, CS/CQD 10%, and CS/CQD 15%), Fig. 7D. Also, the optical image inside Fig. 7D displayed the supernatant of treated films with red blood cells (hemoglobin), which is meaningful in comparison with negative control (PBS). Therefore, the film with low CQDs content (CS/CQD 15%) is the desired candidate for employment as a wound application [42].

Cytotoxicity study

MTT test was carried out to investigate the cytotoxicity of the CS and CS/CQD hydrogel films against HFF-1 cell lines (Fig. 8). The cell viability of samples has remained over 80% even after 48 h. On the other hand, the result shows the cytotoxicity of hydrogels was modestly increased with treated time and the content of CQDs in the nanocomposite. This desired cytocompatibility of the CS/CQD may be related to the good cytotoxicity nature of CS and folic acid-based CQDs reported in the literature [30, 43, 44]. From the results, the constructed CS/CQD films could potentially to suggest a safe wound dressing platform.

Fig. 8
figure 8

Cell viability of HFF-1 cell lines on treating with CS/CQD nanocomposite hydrogel films after different incubation time

Antimicrobial activity

To explain the antimicrobial performance of the CS/CQD nanocomposites, their activity was studied toward E. faecalis, P. aeruginosa, S. mutans, S. aureus, K. pneumoniae, and E. coli bacteria via the disc diffusion procedures. Figure 9 displays that all nanocomposites show significant antibacterial activity compared to CS film as a control sample. The antibacterial performance of constructed CS/CQD nanocomposite hydrogel films is similar due to a low difference between the GM release of samples over 24 h (Table 1). Among different bacterial strains, E. coli shows a lower resistance against nanocomposite. The results of our present work are comparable with the similar hydrogel films in the literatures [39, 45,46,47].

Fig. 9
figure 9

Images of the antibacterial inhibition zones for CS (a), CS/CQD 5% (b), CS/CQD 10% (c), and CS/CQD 15% (d)

Table 1 Inhibition zones (cm) of the tested films toward different bacteria


This study deals a green, simple and economical protocol to prepare an antimicrobial film with great efficiency based on chitosan (CS) hydrogel crosslinked with folic acid-based carbon quantum dots (CQDs) and plasticized by glycerol. Gentamicin (GM) and L-arginine (L-Arg) as antibacterial and bioactive agent are also incorporated in the nanocomposite formulation. The synthesizing CQDs was successfully performed through the hydrothermal technique. The unique functional groups of folic acid-based CQDs, i.e., carboxylate moieties, make it suitable for crosslinking CS via electrostatic interaction. For the construction of hydrogel films, this type of processing offers feasible properties since the availability of starting materials and applications of green approaches, i.e., simple casting and hydrothermal methods. The incorporation of CQDs impacts the polymeric network rigidity (improved the stress and elongation at break values about 2 MPs and 20%, respectively) as it acts as a crosslinker agent. The successful construction of nanocomposites was confirmed with different techniques (FT-IR, UV-Vis, PL, SEM, and AFM analyses). Gentamicin (GM) release from the hydrogel films showed a sustained and controlled release profile over 48 h with a lower initial burst release. This is because of the synergistic effect of CS and CQDs in controlling the release manner in which they enhance the diversity of probable interactions between carrier and GM molecules. Outstandingly, among the nanocomposite, CS/CQD 15% shows the desired blood clotting (about 70%), haemostatic potential (about 34%), notable antibacterial activity toward different bacterial strains (about 2.5 ± 0.1 cm inhibition zones), and cytotoxicity against Human skin fibroblast (HFF-1) cell lines (over 80% cell viability). We believe that the present protocol may receive great interest to prepare nanocomposite hydrogel films as wound dressing bandages through the green protocol to control microbial infection in wound exudate.



Chitosan (99.5%, medium molecular weight), acetic acid (98%), folic acid, L-Arg, Glycerol (99.5%), and all other chemical were procured from Merck Co. All biological agents were supplied from Sigma Aldrich, USA.

Characterization and analysis

UV–vis absorption spectra of the samples were gained with a spectrophotometer (Shimadzu, model 2450). An FTIR spectrometer (Bruker Instruments, model Aquinox 55, Germany) was used to obtained infrared spectra of the samples. Moreover, the surface morphology of the samples was studied using a scanning electron microscope (SEM; LEO, 1430VP) after coating the samples with gold films operating at 5 kV and atomic force microscopy (AFM, FLEX, Nanosurf, Swiss) were used. The fluorescent properties of the films were recorded through a spectrofluorometer (FP-6200, JASCO). Determining the elongation at break and tensile strength of the samples was possible by using a universal testing machine (Zwick/Roell, Model, Z010 Germany).

Synthesis of N-doped CQDs

The N-doped CQDs were synthesized using FA as a bio-precursor through a hydrothermal technique [30]. A well-distributed solution of FA was prepared by dissolving 10 mg of FA in 15 mL ultrapure water and sonicated for 5 min. Subsequently, it is treated in an oven for 8 h at 200 °C for carbonization. As a result of this reaction, a clear light yellow-brown solution was attained. Finally, to remove the precipitate, the solution was centrifuged for 15 min at 11,000 rpm. The supernatant was kept at 0 °C in dark for further use.

Preparation of CS/CQD/GM hydrogel films

1% (w/v) homogenous solution of chitosan was prepared by dissolving a certain amount of chitosan powder (1.000 g) in 0.1% (w/v) acetic acid solution and stirred for 1 h at ambient temperature. Certain amounts of CQDs (0.050, 0.100 and 0.150 g) were dissolved in 20 mL distilled water to prepare 5%, 10%, and 15% solutions (weight% of CQDs to CS content), respectively. Afterward, CQDs and GM (0.010 g) were separately added to the CS solution and stirred until dissolving, followed by the supplementation of L-Arg (0.010 g) to the prepared mixture. Then, as a plasticizer, glycerol (0.5% w/v) was added to the prepared solutions and stirred for 4 h at 50 °C. The formed homogeneous and transparent paste (25 mL) was cast onto a polystyrene plate with a 10 cm diameter and cured at 60 °C for 24 h.

Swelling study

The in-vitro swelling examination was performed at physiological temperature (37 °C) using phosphate-buffered saline (PBS, pH 7.4) solution. Samples (CS/CQD/GM hydrogel films) were weighed (Wi) and socked in 20 mL of the buffered solution. To determine the swelling ratio, samples were taken out at predetermined time intervals and then weighted (Wt) after removing the surface water using a filter paper. The following equation (Eq. 1) was used to determine the swelling ratio:


Drug release study

According to the reported procedure [47], to investigate the drug release profile, each hydrogel film (10 mg) was immersed in phosphate buffered media (10 mL, PBS, pH 7.4) to simulate the physiological microenvironment. Afterward, an aqueous solution (3 mL) of released media was withdrawn and replaced with fresh buffered media at a certain time break. Standard calibration curve for GM at 270 nm used to attained the concentration of drug in the released media. The release profile was considered by the Eq. 2:


Blood clotting

Reported protocols demonstrate the blood clotting assay performance [42]. Sodium citrate as an anticoagulant stabilized the fresh human blood. Initially, all sliced films with 10 mm diameter are located into the 12 well-plates. To pre-warm the films, the plate was placed into an incubator for 15 min at 37 °C. Subsequently, stabilized blood (200 µL) was gradually dropped on the films. Here, the controls group were well with gauze and no sample. Then, to initiate the blood-clotting 40 µL of CaCl2 solution (0.2 M) was added into the blood samples. After incubation for 15 min at 37 °C, the red blood cells are trapped in the clotting. Subsequently, to hemolysis the blood cells, 6 mL of deionized water was added to each well. After 2 min gentle shaking, 2 mL of media was withdrawn and centrifuged for 2 min at 1500 rpm. The absorbance of the supernatant was considered at 540 nm in water using ELISA-reader (BioTech). The blood clotting index (BCI) was measured with the following Eq. 3 for various samples.

$$\text{B}\text{C}\text{I}= \frac{ \text{O}\text{D}\text{S}\text{a}\text{m}\text{p}\text{l}\text{e}}{\text{O}\text{D}\text{C}\text{o}\text{n}\text{t}\text{r}\text{o}\text{l}}$$

where ODsample and ODControl were absorbances of blood that had been in contact with samples and without the sample, respectively.

Hemolysis rate assay

Hemolysis assay was carried out according to the reported procedure with a little modification [42]. At first, the supernatant (plasma) stabilized human fresh blood was taken out by centrifuging. To eliminate lysed hemoglobin, the pellets (human red blood cells) were rinsed 3 times with PBS (pH 7.4). The pellets were immersed in PBS (diluted ten times) and blended with 1 mL PBS having CS/CQD 5%, 10% and 15% and incubated for 60 min at 37 °C. The positive control with 100% hemolysis was obtained by double-distilled water, and the negative control with 0% hemolysis was induced with PBS solution. The incubated samples were centrifuged at 3500 rpm for 10 min. The supernatant was taken to the 96-well plates and their absorbance was measured using a spectrophotometer at 545 nm. The percent of hemolysis rate was determined using the following Eq. 4.

$$\text{Hemolysis Rate}\left(\%\right)=\frac{(\text{Abs sample}\;-\;\text{Abs PBS})}{(\text{Abs water}\;-\;\text{Abs PBS})}\times100$$

Cell culture

Human skin fibroblast (HFF-1) cells were attained from Pasteur Institute of Iran (Tehran, Iran) and cultured in Dulbeccom modified Eagle medium (DMEM, high glucose formulation; Gibco BRL containing 100 mg/mL streptomycin, 100 unit/mL penicillin and 10% (v/v) fetal bovine serum. Cells were seeded in triplicate into 96-well plates at a density of 1 × 104 cells/well and were incubated at 37 °C for 24–48 h (incubator containing 5% CO2) and used in tests when they achieved 80% confluence at logarithmic growing phase.

Cell viability assay

The biocompatibility and the cytotoxicity tests of the nanocomposite hydrogel films were assessed by MTT assay. The films were sterilized with ethanol 70% for 5 min afterward exposure to UV irradiation for 20 min. The samples were washed 2 times with PBS (pH 7.2) and then immersed in a fresh culture medium. After 24 h, HFFF-1 cells were cultured at the density of 104 cells per well into 96-well plates (including samples) in triplicate. As a control group, cells without samples were considered that only received fresh medium. To solubilize the formazan crystals, the medium was replaced with 150 µL fresh culture medium containing MTT solution (5 mg·mL–1) and incubated at 37 °C for 4 h. Then, the medium was taken out and DMSO (200 µL) was added to dissolve the purple-blue MTT formazan precipitate. The absorbance of solubilized formazan was recorded at 570 nm using a multi-well plate reader (Quant Bio-tek Instruments, Winooski, VT, USA) after 30 min and the cell viability was calculated with following Eq. 5.

$$\text{Cell viability}\left(\%\right)=\frac{\text{Mean absorbance of each group}}{\text{Mean absorbance of the control group}}\times100$$

Antimicrobial activity

The antimicrobial performance was carried out with the agar disc diffusion method [48, 49]. To investigate antibacterial activities, the synthesized nanocomposite films were tested against six pathogens and results are presented in Fig. 9; Table 1. The bacteria strains were adjusted to 0.5 McFarland standard and followed by they were treated on the surface of agar plates. Afterward, all films were cut in a sterile condition with 8 mm diameter and were put on agar plates, and then they were incubated at 37 °C. After 18–24 h, the inhibition zones were measured.

Availability of data and materials

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.


  1. Rahimi M, Noruzi EB, Sheykhsaran E, Ebadi B, Kariminezhad Z, Molaparast M, et al. Carbohydrate polymer-based silver nanocomposites: recent progress in the antimicrobial wound dressings. Carbohydr Polym. 2020;231:115696.

    Article  Google Scholar 

  2. Nezhad-Mokhtari P, Javanbakht S, Asadi N, Ghorbani M, Milani M, Hanifehpour Y, et al. Recent advances in honey-based hydrogels for wound healing applications: towards natural therapeutics. J Drug Deliv Sci Technol. 2021;66:102789.

    Article  Google Scholar 

  3. Wenzel R, Perl T. The significance of nasal carriage of Staphylococcus aureus and the incidence of postoperative wound infection. J Hosp Infect. 1995;31(1):13–24.

    Article  Google Scholar 

  4. Puppi D, Chiellini F. Biodegradable polymers for biomedical additive manufacturing. Appl Mater Today. 2020;20:100700.

    Article  Google Scholar 

  5. Javanbakht S, Shaabani A. Multicomponent reactions-based modified/functionalized materials in the biomedical platforms. ACS Appl Bio Mater. 2019;3(1):156–74.

    Article  Google Scholar 

  6. Dhivya S, Padma VV, Santhini E. Wound dressings–a review. BioMedicine. 2015;5(4):1–5.

    Article  Google Scholar 

  7. Simões D, Miguel SP, Ribeiro MP, Coutinho P, Mendonça AG, Correia IJ. Recent advances on antimicrobial wound dressing: a review. Eur J Pharm Biopharm. 2018;127:130–41.

    Article  Google Scholar 

  8. Mohebbi S, Nezhad MN, Zarrintaj P, Jafari SH, Gholizadeh SS, Saeb MR, et al. Chitosan in biomedical engineering: a critical review. Curr Stem Cell Res Therapy. 2019;14(2):93–116.

    Article  Google Scholar 

  9. Fakhri E, Eslami H, Maroufi P, Pakdel F, Taghizadeh S, Ganbarov K, et al. Chitosan biomaterials application in dentistry. Int J Biol Macromol. 2020;162:956–74.

    Article  Google Scholar 

  10. Ahmed F, Soliman FM, Adly MA, Soliman HA, El-Matbouli M, Saleh M. Recent progress in biomedical applications of chitosan and its nanocomposites in aquaculture: a review. Res Vet Sci. 2019;126:68–82.

    Article  Google Scholar 

  11. Ryu JH, Hong S, Lee H. Bio-inspired adhesive catechol-conjugated chitosan for biomedical applications: a mini review. Acta Biomater. 2015;27:101–15.

    Article  Google Scholar 

  12. Rahimi M, Ahmadi R, Kafil HS, Shafiei-Irannejad V. A novel bioactive quaternized chitosan and its silver-containing nanocomposites as a potent antimicrobial wound dressing: structural and biological properties. Mater Sci Engineering: C. 2019;101:360–9.

    Article  Google Scholar 

  13. Pellá MC, Lima-Tenório MK, Tenório-Neto ET, Guilherme MR, Muniz EC, Rubira AF. Chitosan-based hydrogels: from preparation to biomedical applications. Carbohydr Polym. 2018;196:233–45.

    Article  Google Scholar 

  14. Salari M, Khiabani MS, Mokarram RR, Ghanbarzadeh B, Kafil HS. Use of gamma irradiation technology for modification of bacterial cellulose nanocrystals/chitosan nanocomposite film. Carbohydr Polym. 2021;253:117144.

    Article  Google Scholar 

  15. Ebrahimzadeh S, Bari MR, Hamishehkar H, Kafil HS, Lim L-T. Essential oils-loaded electrospun chitosan-poly (vinyl alcohol) nonwovens laminated on chitosan film as bilayer bioactive edible films. LWT. 2021;144:111217.

    Article  Google Scholar 

  16. Mndlovu H, du Toit LC, Kumar P, Choonara YE, Marimuthu T, Kondiah PP, et al. Bioplatform fabrication approaches affecting chitosan-based interpolymer complex properties and performance as wound dressings. Molecules. 2020;25(1):222.

    Article  Google Scholar 

  17. Murugesan S, Scheibel T. Chitosan-based nanocomposites for medical applications. J Polym Sci. 2021;59(15):1610–42.

    Article  Google Scholar 

  18. Campea MA, Majcher MJ, Lofts A, Hoare T. A review of design and fabrication methods for nanoparticle network hydrogels for biomedical, environmental, and industrial applications. Adv Funct Mater. 2021;31:2102355.

    Article  Google Scholar 

  19. Biondi M, Borzacchiello A, Mayol L, Ambrosio L. Nanoparticle-integrated hydrogels as multifunctional composite materials for biomedical applications. Gels. 2015;1(2):162–78.

    Article  Google Scholar 

  20. Javanbakht S, Shaabani A. Encapsulation of graphene quantum dot-crosslinked chitosan by carboxymethylcellulose hydrogel beads as a pH-responsive bio-nanocomposite for the oral delivery agent. Int J Biol Macromol. 2019;123:389–97.

    Article  Google Scholar 

  21. Javanbakht S, Nabi M, Shaabani A. Graphene quantum dots-crosslinked gelatin via the efficient Ugi four-component reaction: safe photoluminescent implantable carriers for the pH-responsive delivery of doxorubicin. Materialia. 2021;20:101233.

    Article  Google Scholar 

  22. Su W, Wang R, Qian C, Li X, Tong Q, Jiao T. Research progress review of preparation and applications of fluorescent hydrogels. J Chem. 2020;2020:8246429.

  23. Li Y, Young DJ, Loh XJ. Fluorescent gels: a review of synthesis, properties, applications and challenges. Mater Chem Front. 2019;3(8):1489–502.

    Article  Google Scholar 

  24. Javanbakht S, Namazi H. Doxorubicin loaded carboxymethyl cellulose/graphene quantum dot nanocomposite hydrogel films as a potential anticancer drug delivery system. Mater Sci Engineering: C. 2018;87:50–9.

    Article  Google Scholar 

  25. Javanbakht S, Nazari N, Rakhshaei R, Namazi H. Cu-crosslinked carboxymethylcellulose/naproxen/graphene quantum dot nanocomposite hydrogel beads for naproxen oral delivery. Carbohydr Polym. 2018;195:453–9.

    Article  Google Scholar 

  26. Molaei MJ. A review on nanostructured carbon quantum dots and their applications in biotechnology, sensors, and chemiluminescence. Talanta. 2019;196:456–78.

    Article  Google Scholar 

  27. Pandey H, Khare P, Singh S, Singh SP. Carbon nanomaterials integrated molecularly imprinted polymers for biological sample analysis: a critical review. Mater Chem Phys. 2020;239:121966.

    Article  Google Scholar 

  28. Molaei MJ. Carbon quantum dots and their biomedical and therapeutic applications: a review. RSC Adv. 2019;9(12):6460–81.

    Article  Google Scholar 

  29. Javanbakht S, Shaabani A. Stimuli-Responsive Bio‐Based Quantum Dots in Biomedical Applications. Nanoengineering of Biomaterials: Biomedical Applications. 2022;2:323–52.

    Article  Google Scholar 

  30. Liu H, Li Z, Sun Y, Geng X, Hu Y, Meng H, et al. Synthesis of luminescent Carbon dots with Ultrahigh Quantum yield and inherent folate receptor-positive Cancer Cell Targetability. Sci Rep. 2018;8(1):1086.

    Article  Google Scholar 

  31. Das R, Bandyopadhyay R, Pramanik P. Carbon quantum dots from natural resource: a review. Mater today Chem. 2018;8:96–109.

    Article  Google Scholar 

  32. Javanbakht S, Saboury A, Shaabani A, Mohammadi R, Ghorbani M. Doxorubicin imprinted photoluminescent polymer as a pH-responsive nanocarrier. ACS Appl Bio Mater. 2020;3(7):4168–78.

    Article  Google Scholar 

  33. Smith AD, Kim Y-I, Refsum H. Is folic acid good for everyone? Am J Clin Nutr. 2008;87(3):517–33.

    Article  Google Scholar 

  34. Zhu C, Yang S, Wang G, Mo R, He P, Sun J, et al. A new mild, clean and highly efficient method for the preparation of graphene quantum dots without by-products. J Mater Chem B. 2015;3(34):6871–6.

    Article  Google Scholar 

  35. Javanbakht S, Namazi H. Solid state photoluminescence thermoplastic starch film containing graphene quantum dots. Carbohydr Polym. 2017;176:220–6.

    Article  Google Scholar 

  36. Yadollahi M, Namazi H, Barkhordari S. Preparation and properties of carboxymethyl cellulose/layered double hydroxide bionanocomposite films. Carbohydr Polym. 2014;108:83–90.

    Article  Google Scholar 

  37. Rakhshaei R, Namazi H. A potential bioactive wound dressing based on carboxymethyl cellulose/ZnO impregnated MCM-41 nanocomposite hydrogel. Mater Sci Engineering: C. 2017;73:456–64.

    Article  Google Scholar 

  38. Nair A, Haponiuk JT, Thomas S, Gopi S. Natural carbon-based quantum dots and their applications in drug delivery: a review. Biomed Pharmacother. 2020;132:110834.

    Article  Google Scholar 

  39. Liu Y, Ji P, Lv H, Qin Y, Deng L. Gentamicin modified chitosan film with improved antibacterial property and cell biocompatibility. Int J Biol Macromol. 2017;98:550–6.

    Article  Google Scholar 

  40. Qiu Y, Xu D, Sui G, Wang D, Wu M, Han L, et al. Gentamicin decorated phosphatidylcholine-chitosan nanoparticles against biofilms and intracellular bacteria. Int J Biol Macromol. 2020;156:640–7.

    Article  Google Scholar 

  41. Mehrabani MG, Karimian R, Rakhshaei R, Pakdel F, Eslami H, Fakhrzadeh V, et al. Chitin/silk fibroin/TiO2 bio-nanocomposite as a biocompatible wound dressing bandage with strong antimicrobial activity. Int J Biol Macromol. 2018;116:966–76.

    Article  Google Scholar 

  42. Rahimi M, Ahmadi R, Samadi Kafil H, Shafiei-Irannejad V. A novel bioactive quaternized chitosan and its silver-containing nanocomposites as a potent antimicrobial wound dressing: structural and biological properties. Mater Sci Eng C Mater Biol Appl. 2019;101:360–9.

    Article  Google Scholar 

  43. Abdull Rasad MSB, Halim AS, Hashim K, Rashid AHA, Yusof N, Shamsuddin S. In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts. Carbohydr Polym. 2010;79(4):1094–100.

    Article  Google Scholar 

  44. Kandra R, Bajpai S. Synthesis, mechanical properties of fluorescent carbon dots loaded nanocomposites chitosan film for wound healing and drug delivery. Arab J Chem. 2020;13(4):4882–94.

    Article  Google Scholar 

  45. Fan L, Cheng C, Qiao Y, Li F, Li W, Wu H, et al. GNPs-CS/KGM as hemostatic first aid wound dressing with antibiotic effect: in vitro and in vivo study. PLoS One. 2013;8(7):e66890.

    Article  Google Scholar 

  46. Raphael KJ, Meimandipour A. Antimicrobial activity of Chitosan Film forming solution enriched with essential oils; an in Vitro Assay. Iran J Biotechnol. 2017;15(2):111–9.

    Article  Google Scholar 

  47. Javanbakht S, Nabi M, Shadi M, Amini MM, Shaabani A. Carboxymethyl cellulose/tetracycline@ UiO-66 nanocomposite hydrogel films as a potential antibacterial wound dressing. Int J Biol Macromol. 2021;188:811–9.

    Article  Google Scholar 

  48. Kazeminava F, Arsalani N, Ahmadi R, Kafil HS, Geckeler KE. A facile approach to incorporate silver nanoparticles into solvent-free synthesized PEG-based hydrogels for antibacterial and catalytical applications. Polym Test. 2021;101:106909.

    Article  Google Scholar 

  49. Arsalani N, Kazeminava F, Akbari A, Hamishehkar H, Jabbari E, Kafil HS. Synthesis of polyhedral oligomeric silsesquioxane nano-crosslinked poly (ethylene glycol)‐based hybrid hydrogels for drug delivery and antibacterial activity. Polym Int. 2019;68(4):667–74.

    Article  Google Scholar 

Download references


This study was supported by Maragheh University of Medical Sciences, Maragheh, Iran. We thank all staff of DARC for their collaboration.


This study was supported by Maragheh University of Medical sciences, Maragheh, Iran.

Author information

Authors and Affiliations



H.S.K and F.K and A.T did study design, F.K and S.J and P.G and H.S.K and P NM did sample preparation and analysis, P.G and M.N. and F.K prepared figures of the manuscript. F.K. and H.S.K and S.J wrote the main manuscript and All authors reviewed the manuscript. The author(s) read and approved the final manuscript.

Corresponding authors

Correspondence to Asghar Tanomand or Hossein Samadi Kafil.

Ethics declarations

Ethics approval and consent to participate

This study was approved by local ethic committee of Maragheh University of Medical Sciences with reference number IR.MUMS.REC.1397.1320. All steps were done according to Helsinki declaration and under consideration by Biosafety committee and assessment.

Competing interests

There are no conflicts to declare.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kazeminava, F., Javanbakht, S., Nouri, M. et al. Gentamicin-loaded chitosan/folic acid-based carbon quantum dots nanocomposite hydrogel films as potential antimicrobial wound dressing. J Biol Eng 16, 36 (2022).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: