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  1. Research

    CE-UV/VIS and CE-MS for monitoring organic impurities during the downstream processing of fermentative-produced lactic acid from second-generation renewable feedstocks

    During the downstream process of bio-based bulk chemicals, organic impurities, mostly residues from the fermentation process, must be separated to obtain a pure and ready-to-market chemical. In this study, cap...

    Hendrik Laube, Frank-Michael Matysik, Andreas Schmidberger, Kerstin Mehlmann, Andreas Toursel and Jana Boden

    Journal of Biological Engineering 2016 10:7

    Published on: 19 May 2016

  2. Review

    Respirator masks protect health but impact performance: a review

    Respiratory protective masks are used whenever it is too costly or impractical to remove airborne contamination from the atmosphere. Respirators are used in a wide range of occupations, form the military to me...

    Arthur T. Johnson

    Journal of Biological Engineering 2016 10:4

    Published on: 9 February 2016

  3. Research

    End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis

    Synthetic biology aims to engineer biological systems for desired behaviors. The construction of these systems can be complex, often requiring genetic reprogramming, extensive de novo DNA synthesis, and functi...

    Gregory Linshiz, Erik Jensen, Nina Stawski, Changhao Bi, Nick Elsbree, Hong Jiao, Jungkyu Kim, Richard Mathies, Jay D. Keasling and Nathan J. Hillson

    Journal of Biological Engineering 2016 10:3

    Published on: 2 February 2016

  4. Erratum

    Erratum to: No training required: experimental tests support homology-based DNA assembly as a best practice in synthetic biology

    Afnan Azizi, Wilson Lam, Hilary Phenix, Lioudmila Tepliakova, Ian J. Roney, Daniel Jedrysiak, Alex Power, Vaibhav Gupta, Nada Elnour, Martin Hanzel, Alexandra C. Tzahristos, Shihab Sarwar and Mads Kærn

    Journal of Biological Engineering 2016 10:1

    Published on: 7 January 2016

    The original article was published in Journal of Biological Engineering 2015 9:8

  5. Research

    Rational design of Raman-labeled nanoparticles for a dual-modality, light scattering immunoassay on a polystyrene substrate

    Surface-enhanced Raman scattering (SERS) is a powerful light scattering technique that can be used for sensitive immunoassay development and cell labeling. A major obstacle to using SERS is the complexity of f...

    Nathan D. Israelsen, Donald Wooley, Cynthia Hanson and Elizabeth Vargis

    Journal of Biological Engineering 2016 10:2

    Published on: 7 January 2016

  6. Research

    Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme

    Plant terpenoids are known for their diversity, stereochemical complexity, and their commercial interest as pharmaceuticals, food additives, and cosmetics. Developing biotechnology approaches for the productio...

    Thiyagarajan Gnanasekaran, Konstantinos Vavitsas, Johan Andersen-Ranberg, Agnieszka Zygadlo Nielsen, Carl Erik Olsen, Björn Hamberger and Poul Erik Jensen

    Journal of Biological Engineering 2015 9:24

    Published on: 22 December 2015

  7. Research

    Stimulating effect of graphene oxide on myogenesis of C2C12 myoblasts on RGD peptide-decorated PLGA nanofiber matrices

    In the field of biomedical engineering, many studies have focused on the possible applications of graphene and related nanomaterials due to their potential for use as scaffolds, coating materials and delivery ...

    Yong Cheol Shin, Jong Ho Lee, Min Jeong Kim, Suck Won Hong, Bongju Kim, Jung Keun Hyun, Yu Suk Choi, Jong-Chul Park and Dong-Wook Han

    Journal of Biological Engineering 2015 9:22

    Published on: 25 November 2015

  8. Research

    Collective cell migration of smooth muscle and endothelial cells: impact of injury versus non-injury stimuli

    Cell migration is a vital process for growth and repair. In vitro migration assays, utilized to study cell migration, often rely on physical scraping of a cell monolayer to induce cell migration. The physical act...

    Kaitlyn R. Ammann, Katrina J. DeCook, Phat L. Tran, Valerie M. Merkle, Pak K. Wong and Marvin J. Slepian

    Journal of Biological Engineering 2015 9:19

    Published on: 15 October 2015

  9. Research

    Patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells

    The interaction of stem cells with their culture substrates is critical in controlling their fate and function. Declining stemness of adult-derived human mesenchymal stem cells (hMSCs) during in vitro expansion o...

    Lucas H. Hofmeister, Lino Costa, Daniel A. Balikov, Spencer W. Crowder, Alexander Terekhov, Hak-Joon Sung and William H. Hofmeister

    Journal of Biological Engineering 2015 9:18

    Published on: 12 October 2015

  10. Letters to the Editor

    Standards not that standard

    There is a general assent on the key role of standards in Synthetic Biology. In two consecutive letters to this journal, suggestions on the assembly methods for the Registry of standard biological parts have b...

    Cristina Vilanova, Kristie Tanner, Pedro Dorado-Morales, Paula Villaescusa, Divya Chugani, Alba Frías, Ernesto Segredo, Xavier Molero, Marco Fritschi, Lucas Morales, Daniel Ramón, Carlos Peña, Juli Peretó and Manuel Porcar

    Journal of Biological Engineering 2015 9:17

    Published on: 1 October 2015

  11. Methodology

    Development of a miniaturized stimulation device for electrical stimulation of cells

    Directing cell behaviour using controllable, on-demand non-biochemical methods, such as electrical stimulation is an attractive area of research. While there exists much potential in exploring different modes ...

    Gordon Minru Xiong, Anh Tuan Do, Jun Kit Wang, Chee Leong Yeoh, Kiat Seng Yeo and Cleo Choong

    Journal of Biological Engineering 2015 9:14

    Published on: 4 September 2015

  12. Methodology

    Essential validation methods for E. coli strains created by chromosome engineering

    Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a controlled fashion. Such techniques are widely used ...

    Sriram Tiruvadi Krishnan, M. Charl Moolman, Theo van Laar, Anne S. Meyer and Nynke H. Dekker

    Journal of Biological Engineering 2015 9:11

    Published on: 1 July 2015

  13. Letters to the Editor

    No training required: experimental tests support homology-based DNA assembly as a best practice in synthetic biology

    The Registry of Standard Biological Parts imposes sequence constraints to enable DNA assembly using restriction enzymes. Alnahhas et al. (Journal of Biological Engineering 2014, 8:28) recently argued that thes...

    Afnan Azizi, Wilson Lam, Hilary Phenix, Lioudmila Tepliakova, Ian J Roney, Daniel Jedrysiak, Alex Power, Vaibhav Gupta, Nada Elnour, Martin Hanzel, Alexandra C Tzahristos, Shihab Sarwar and Mads Kærn

    Journal of Biological Engineering 2015 9:8

    Published on: 12 June 2015

    The Erratum to this article has been published in Journal of Biological Engineering 2016 10:1

  14. Methodology

    Chemical lysis of cyanobacteria

    We have developed a mixture of enzymes and chemicals that completely lyse cyanobacteria. Since the treatment involves only readily-available chemicals and simple proteins that degrade the components of the cya...

    Kunal K. Mehta, Niklaus H. Evitt and James R. Swartz

    Journal of Biological Engineering 2015 9:10

    Published on: 5 June 2015

  15. Research

    Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors

    Conventional experiments in small scale are often performed in a ‘Black Box’ fashion, analyzing only the product concentration in the final sample. Online monitoring of relevant process characteristics and par...

    S. J. Wewetzer, M. Kunze, T. Ladner, B. Luchterhand, S. Roth, N. Rahmen, R. Kloß, A. Costa e Silva, L. Regestein and J. Büchs

    Journal of Biological Engineering 2015 9:9

    Published on: 30 May 2015

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