- Open Access
Synthetic feedback control using an RNAi-based gene-regulatory device
© Bloom et al.; licensee BioMed Central. 2015
- Received: 10 November 2014
- Accepted: 20 March 2015
- Published: 14 April 2015
Homeostasis within mammalian cells is achieved through complex molecular networks that can respond to changes within the cell or the environment and regulate the expression of the appropriate genes in response. The development of biological components that can respond to changes in the cellular environment and interface with endogenous molecules would enable more sophisticated genetic circuits and greatly advance our cellular engineering capabilities.
Here we describe a platform that combines a ligand-responsive ribozyme switch and synthetic miRNA regulators to create an OFF genetic control device based on RNA interference (RNAi). We developed a mathematical model to highlight important design parameters in programming the quantitative performance of RNAi-based OFF control devices. By modifying the ribozyme switch integrated into the system, we demonstrated RNAi-based OFF control devices that respond to small molecule and protein ligands, including the oncogenic protein E2F1. We utilized the OFF control device platform to build a negative feedback control system that acts as a proportional controller and maintains target intracellular protein levels in response to increases in transcription rate.
Our work describes a novel genetic device that increases the level of silencing from a miRNA in the presence of a ligand of interest, effectively creating an RNAi-based OFF control device. The OFF switch platform has the flexibility to be used to respond to both small molecule and protein ligands. Finally, the RNAi-based OFF switch can be used to implement a negative feedback control system, which maintains target protein levels around a set point level. The described RNAi-based OFF control device presents a powerful tool that will enable researchers to engineer homeostasis in mammalian cells.
- Feedback control
- Synthetic biology
The complexity of mammalian cells is largely due to the sophisticated control mechanisms employed to modulate the expression of endogenous genes . Molecules in the cell are organized into networks, which can respond to changes in intracellular protein or metabolite levels, as well as changes in the environment, by upregulating or downregulating the expression of any number of genes . These genetic circuits are essential for virtually all cellular processes including differentiation , replication , and signal transduction . The ability to interface with these endogenous programs to create novel molecular networks would greatly advance our cellular engineering capabilities for a variety of applications. Central to our ability to create these new genetic circuits is the development of biological components that can respond to changes in the cellular environment and interface with endogenous molecules to enable more sophisticated synthetic circuits .
MicroRNAs (miRNA) are a class of small regulatory RNAs that are used to post-transcriptionally control gene expression in higher eukaryotes . MiRNA biogenesis begins with longer pri-miRNAs, which are cleaved by a Microprocessor complex into shorter ~60 nucleotide pre-miRNA hairpins and subsequently exported from the nucleus. The pre-miRNA is subject to a final cleavage step in the cytoplasm by the enzyme Dicer, which creates a 22–25 nucleotide (nt) double stranded RNA . One of these RNA strands, called the guide strand, is then incorporated into one of the Argonaut family of proteins to create the RNA-induced silencing complex (RISC). This complex subsequently cleaves or translationally represses the target transcript depending on the degree of complementarity between the miRNA and the target . This powerful mechanism of gene regulation plays vital roles in diverse processes, such as development and angiogenesis, and it is estimated that RNAi regulates the majority of genes in the human genome .
RNA-based synthetic biology tools are powerful resources for building synthetic control systems that can detect and respond to changes in cell state or the cellular environment, and synthetic RNA-based systems have been demonstrated in mammalian cells [9-12]. Frameworks have been described for the design of miRNA- and shRNA-based molecular switches, whose biogenesis is responsive to changes in concentration of intracellular ligand molecules . In general, these regulators are constructed by coupling the binding of a ligand to an RNA aptamer with the proper formation and biogenesis of the RNAi element . The physical coupling of the aptamer to the RNAi element has been accomplished in several ways. Researchers have directly coupled an aptamer to a pri-miRNA, such that binding of the ligand to the aptamer sterically inhibits proper biogenesis by the Microprocessor . Both direct coupling and strand displacement mechanisms have been described, where ligand binding to the aptamer changes the structure of an shRNA such that it is unable to be properly processed by Dicer [14,15].
These RNAi-based control systems are particularly useful for imparting conditional silencing over endogenous genes. Previously, we developed a computational model that predicts target gene expression levels resulting from synthetic circuits based on miRNA regulatory components . This model was applied to direct the rational design of miRNA-based genetic circuits and supported an in-vivo protein concentration detector circuit by providing a quantitative relationship between target reporter and protein ligand levels in the cell. However, the designs in this previous work utilize a switch architecture that inhibits RNAi-based gene silencing in the presence of the ligand, thereby increasing target gene expression as a function of increasing ligand concentration and operating as ON switches. An RNAi-based control platform that can be used to decrease the expression of a target gene in response to increasing concentrations of proteins of interest has not been described. However, this type of OFF switch regulation is essential for building core control strategies such as negative feedback control. Negative feedback is a prevalent control mechanism found in diverse biological systems, such as bacterial chemotaxis  and vision , that can be utilized to achieve homeostasis.
Here we describe and characterize an OFF genetic control device based on RNAi that combines a ligand-responsive ribozyme switch and synthetic miRNA regulators. The device architecture links ribozyme cleavage to miRNA levels, such that ligand binding modulates ribozyme cleavage rates and thus miRNA-based gene silencing. The system was prototyped using a previously described theophylline-responsive ribozyme switch and a miRNA that targets a fluorescent reporter. A mathematical model of this system was developed to highlight important design parameters in programming the quantitative performance of RNAi-based OFF control devices. We demonstrated RNAi-based OFF control devices that respond to protein ligands by incorporating protein-responsive ribozyme switches to E2F1 and MS2. We utilized the OFF control device platform to build a negative feedback control system that maintains target intracellular protein levels around a set point. The negative feedback control system acts as a proportional controller, maintaining target intracellular protein levels in response to increases in transcription rate. The described RNAi-based OFF control device presents a powerful tool that will enable researchers to engineer homeostasis into mammalian cells, for example, to maintain a desired phenotype even in the presence of genetic mutations or fluctuating levels of signaling molecules or cytokines.
A trans-acting genetic device that exhibits OFF control by coupling ribozyme switches and miRNAs
We used a modular ligand-responsive ribozyme switch platform that supports efficient tailoring of RNA device function . The ribozyme switch platform specifies physical linkages between three functional RNA components: a sensor encoded by an RNA aptamer, an actuator encoded by a hammerhead ribozyme, and a transmitter encoded by a sequence that undergoes a strand-displacement event (Additional file 1: Figure S1). The modular nature of this ribozyme design makes it relatively straightforward to modify the ligand-responsiveness of the switch by swapping in new aptamer sequences into the sensor component of the platform while maintaining the overall activity of the switch.
Device performance was quantified by measuring target protein (GFP) and mature miRNA levels in the presence and absence of ligand (Figure 2b, c). GFP levels were measured using a flow cytometry assay and mature miRNA levels were measured using a qRT-PCR assay with custom TaqMan small RNA probes. The data demonstrate that the longest spacer tested (2500 bp) resulted in the greatest change in target gene expression levels in the presence and absence of ligand (Figure 2b). qRT-PCR analysis showed that the decrease in target gene expression levels correlated with an increase in mature miRNA levels, indicating that the observed changes in target gene expression levels were due to miRNA silencing (Figure 2c). The OFF state (i.e., expression level in the presence of theophylline) measured for all constructs is similar to that from the inactive ribozyme control construct, which sets the expected lower limit for the system (Figure 2b). The ON state (i.e., expression level in the absence of theophylline) generally increased with spacer length, consistent with previous findings in the literature . Overall, the device with the longest spacer length tested (2500 bp) exhibited ON and OFF states very close to the controls (Figure 2b, Additional file 1: Figure S2), indicating that this device was accessing nearly the full potential dynamic range of the system. The data indicate that longer spacer lengths may increase the time between transcription of the ribozyme component and transcription of the downstream miRNA component, thus increasing the probability that the ribozyme will cleave and abort transcription  prior to the transcription and processing of the miRNA.
A computational model informs the design of the RNAi-based OFF control device
We developed a mathematical model to inform the design of the RNAi-based OFF control device to elucidate important system design parameters. The model describes the kinetics of ribozyme cleavage and miRNA formation using a series of differential equations describing the separate processes (Additional file 1: Figure S3, Additional file 1: Text S1). The transcription of the ribozyme-miRNA transcript is modeled as a two-step process in which an “immature transcript”, harboring only the ribozyme component, is initially formed. This immature transcript is either cleaved and subsequently degraded or elongated into a full-length transcript, encoding both the ribozyme and miRNA components. Mature miRNAs are formed from the full-length transcript and subsequently silence target gene expression through enzymatic degradation using a term that resembles traditional Michaelis-Menten kinetics, which includes a constant (K m ) describing the affinity between the miRNA and the target transcript . The presence of the input ligand will lower the ribozyme cleavage rate, resulting in an increase in the level of full-length transcript and thus increased miRNA levels. The binding kinetics between the aptamer and the ligand (k a , k d ) as well as the cleavage rates of the ribozyme when bound and unbound to the input ligand (k cleave-bound , k cleave ) were measured experimentally [23,24]. The parameters that determine the gene silencing at different miRNA levels (a, K m ) were determined previously . All other model parameters were determined using parameter fitting software in MATLAB by simulating the system of equations and fitting the steady-state values to the experimental data for the theophylline-responsive OFF control device.
The model can be used to identify which components of the circuit can be targeted for modification to optimize the performance of the circuit. For example, both spacer length and the ratio of ribozyme cleavage rates in the presence and absence of the ligand (k cleave /k cleave-bound ) were found to be critical parameters in tuning the dynamic range of the OFF control device. High-throughput screening strategies have been described that support efficient tuning of the cleavage rates of ribozyme switches . As another example, the model indicated that miRNA silencing efficiency, largely determined by K m , determines the silencing observed at a given miRNA level. The K m of a miRNA-target gene pair can be modified by incorporating additional target sites into the 3′ untranslated region (UTR) of the target transcript [22,26]. With the components selected for this study, the systems were limited to dynamic ranges on the order of ~2-fold. While this regulatory activity is on the order of that observed with endogenous miRNA regulatory networks , it is lower than many synthetic transcription-based control systems in use. Improvements in the properties of the underlying device components (i.e., ribozyme switch, miRNA) will allow for OFF control devices with improved dynamic ranges supporting broader applications.
Protein-responsive RNAi-based OFF control devices
To adapt the RNAi-based OFF control devices to respond to proteins, we replaced the small molecule-responsive ribozyme switch with ribozymes that alter their cleavage activity in the presence of specified protein ligands. The modular nature of the ribozyme switch platform allowed us to tailor the ligand responsiveness of the switch by swapping new aptamer sequences into the sensor domain of the platform .
We developed protein-responsive ribozyme switches to two protein ligands: the MS2 bacteriophage coat protein (MS2) and an oncogene (E2F1). Specifically, sequences for previously characterized RNA aptamers to MS2  and E2F1  were placed directly into the sensor domain of the ribozyme switch platform using a previously characterized transmitter sequence . The binding and cleavage activities of the MS2- and E2F1-responsive ribozyme switches were characterized using previously described in vitro assays. The binding activities of the switches were measured using a surface plasmon resonance assay on a Biacore instrument . The results of this binding assay indicate that the protein-responsive switches bind their cognate ligands with high affinities; the MS2-responsive switch has a KD of 296 nM  and the E2F1-responsive switch has a KD of 548 nM (Additional file 1: Figure S4a). The cleavage rate of the MS2-responsive switch was measured using a gel-based cleavage assay . The results of the cleavage assay demonstrate that the MS2 ligand substantially affected the cleavage rate of the ribozyme switch, resulting in an approximate 2.5-fold decrease in cleavage rate in the presence of 2 μM MS2 . End point cleavage assays were similarly performed on the E2F1-responsive switch and confirmed that the presence of ligand decreased the cleavage activity of this ribozyme switch (Additional file 1: Figure S4b, c). Taken together, the data indicate that the protein-responsive ribozyme switches bind their cognate ligands with high affinities and in a manner that disrupts cleavage activity of the ribozyme.
Device performance was quantified by measuring target protein (GFP) and mature miRNA levels in the presence and absence of the protein ligands as previously described (Figure 4b-e). Plasmids expressing MS2 or E2F1 were transiently transfected into the stable cell lines expressing the MS2- or E2F1-responsive OFF control devices, respectively, and compared to the identical stable cell line transfected with a mock plasmid (i.e., no protein ligand expressed). The data demonstrate that GFP expression levels are reduced up to 2.1- and 1.8-fold in response to MS2 and E2F1 expression, respectively (Figure 4b,c). As with the theophylline-responsive OFF control device, the OFF states of the protein-responsive devices are similar to the positive control (i.e., miRNA only). When taking into account the differences in cleavage rates (k cleave /k cleave-bound ) between the protein-responsive ribozymes and the theophylline-responsive ribozyme, with no additional refitting of other parameters, the model predicts the observed difference in gene-regulatory activities of the devices (Figure 4d). Larger changes in target gene expression correlated with larger cleavage rate ratios in the presence and absence of ligand, supporting that the cleavage rate ratio is a key design parameter for these systems. The qRT-PCR analysis indicated that the increase in GFP silencing was due to an increase in the levels of mature miRNAs within the cells (Figure 4e). Taken together, these results indicate that OFF control devices can be constructed to respond to a variety of ligands by integrating ribozyme switches that respond to the ligands of interest.
Protein-responsive RNAi-based OFF control devices support the implementation of negative feedback control systems
Feedback regulation is ubiquitous in biology and fundamental to many engineering applications. Synthetic biologists have designed a wide range of engineered biological transcriptional feedback regulation systems [31,32]. Negative and positive transcriptional feedback controllers have been used to stabilize desired output protein concentrations, accelerate system dynamics, and generate biological oscillations [33-35]. However, engineered genetic negative feedback control systems have been used to regulate the expression of transgenes; a system has not been developed that can exert feedback control over endogenous genes. This expanded capability would be a powerful tool to engineer synthetic circuits to maintain desired cell phenotypes, for example, in the presence of genetic mutations or fluctuating levels of signaling molecules or cytokines.
The RNAi-based OFF control device has the ability to regulate the levels of a target protein around a desired set point by enabling the implementation of a negative feedback control system in mammalian cells (Additional file 1: Text S1). In this system design, a ribozyme switch responsive to a protein of interest and a miRNA that targets that same protein of interest are integrated into the OFF control device architecture. Thus, an increase in levels of the input protein will result in an increase in levels of the miRNA, which will subsequently reduce levels of the protein to the desired set point level.
We examined the model predictions in our experimental system by modifying the OFF control device to exhibit negative feedback around the levels of the MS2 protein (Figure 5c). An expression cassette was designed in which the MS2-DsRed fusion protein was under the transcriptional control of a doxycycline-inducible CMV-TetO2 promoter and TetR transcriptional repressor pair. In a separate expression cassette, the MS2-responsive OFF control device was modified such that the GFP-targeting miRNA component was replaced with a DsRed-targeting miRNA. In this negative feedback system design, the MS2-DsRed protein levels serve as the system output. As the output levels increase, the MS2 will trigger the formation of miRNAs that target the coding region of DsRed, thereby acting to reduce the levels of MS2-DsRed and serving as a form of negative feedback control. Isogenic cell lines stably expressing both expression cassettes were generated from a Flp-In HEK293 T-REx cell line as previously described. The Flp-In HEK293 T-REx cell line also stably expresses the TetR transcriptional repressor, such that the maximum level of MS2-DsRed (the input) is tightly regulated by the concentration of doxycycline in the media. In particular, the levels of MS2-DsRed were held low prior to induction and measurements were made 48 hours after induction, such that differences in the half lives of d2EGFP and MS2-DsRed (2 hours  versus 8 hours , respectively) would not affect the experimental results.
The resulting stable cell lines were used to characterize the activity of the negative feedback control device regulating MS2 levels (Figure 5c). DsRed levels were used as the output from the feedback system and were measured using a flow cytometry assay, the input (maximum MS2-DsRed level) was controlled using a doxycycline-responsive inducible promoter. Our data show that, compared to a system with no feedback control, there is a significant reduction in the levels of MS2 protein as the transcription rate is increased (Figure 5d). The local slope was also calculated as: Slope i = (Output i – Output i-1 ) /(Input i – Input i-1 ) (Figure 5e). Our experimental system (Figure 5d, e) exhibits similar behavior to that predicted of a proportional controller (Figure 5b) in that the output is not independent of the input, but rather linearly increases as the input increases. Our results highlight that the RNAi-based OFF control device platform can be used to effectively exert feedback control over the levels of a protein of interest.
While there are several examples of negative feedback being implemented in synthetic circuits to alter the dynamics of the circuit [34,38], as well as in the construction of oscillators  and toggle switches , the described control systems do not have the ability to exert feedback control over endogenous genes. Our RNAi-based feedback control system behaves as a proportional controller (P-controller), where the control action (miRNA level) is proportional to the output (protein level). To implement integral feedback control the control action (miRNA level) must be solely a function of the output (protein level). Our model indicates that perfect adaptation cannot be achieved in our system as the miRNA degradation rate is a linear function of the miRNA level. Maintaining miRNA degradation at a constant level would allow us to achieve perfect adaptation . However, in practice, keeping the rate of change of a molecular species independent of its own level through zeroth-order degradation kinetics is difficult to achieve in cellular systems, where molecular concentrations are governed by biochemical kinetics. Any mechanisms working to decrease the concentration of the control action species will likely need to operate at saturation with respect to the control action species in order to achieve zeroth order degradation kinetics. It is likely that many biological feedback systems operate using proportional control and may need to incorporate additional redundancies in cases where maintaining the level of a molecule within a narrow concentration range is critical.
Our work describes a novel genetic device that increases the level of silencing from a miRNA in the presence of a ligand of interest, effectively creating an RNAi-based OFF control device. Our OFF control device incorporates a ligand-responsive ribozyme and spacer element upstream of a miRNA regulator within the same expression cassette such that co-transcriptional cleavage of the ribozyme aborts transcription and prevents pri-miRNA formation. In the presence of ligand, the cleavage rate of the ribozyme is reduced, resulting in accumulation of pri-miRNA transcripts, which are subsequently processed into mature miRNAs that silence the target gene. Similar to previous designs , this coupled architecture allows the miRNA regulator to act as a genetic inverter, by inverting the signal from the ON ribozyme switch to an OFF genetic switch. We demonstrated the flexibility of the OFF switch platform by showing that it can be used to respond to both small molecule and protein ligands and developed a computational model describing the device that revealed important design parameters and parameter ranges associated with optimal performance of the control device. Finally, we used the RNAi-based OFF switch to implement a negative feedback control system, that maintained target intracellular protein levels in response to increases in transcription rate.
Our computational model revealed important design parameters and parameter ranges associated with optimal performance of these control devices. Thus, the model can be used to guide careful selection or tuning of components and/or inform how different parameters would impact the quantitative behavior of the control device. For example, the model identified spacer length, ribozyme cleavage rates in the presence and absence of ligand, and miRNA silencing efficiency as key parameters in the design of OFF RNAi-based control devices.
With the components selected for this study, the systems were limited to dynamic ranges on the order of ~2-fold. While this regulatory activity is on the order of that observed with endogenous miRNA regulatory networks , improvements to these activities will increase the broader utility of these devices in synthetic contexts. Future improvements to the dynamic ranges exhibited by OFF RNAi-based control devices can be achieved through refinement of the underlying device components (i.e., ribozyme switch, miRNA).
By leveraging an RNAi-based regulatory mechanism, the described OFF control device can be adapted to exert negative feedback control over endogenous gene targets. The described control platform can be used in future cellular engineering and gene therapy applications, where the expression of key genes can be kept at desired levels even in the presence of cellular mutations or malfunctioning signaling pathways. Further development and optimization of the ribozyme switch and miRNA components will enable researchers to extend this platform for implementation into applications in health and medicine.
Modern molecular biology techniques were used to create the plasmids and cell lines used in this work. A chemically-competent Escherichia coli strain TOP10 was used for cloning purposes (Life Technologies, Carlsbad, CA) and was grown in LB medium (Becton, Dickinson and Company (BD), Franklin Lakes, NJ) with the indicated antibiotic concentrations. Custom oligonucleotides were synthesized by Integrated DNA Technologies (IDT, Coralville, IA) and the Stanford Protein and Nucleic Acid Facility (PAN, Stanford, CA). PCR was performed with Taq polymerase (New England Biolabs, Ipswich, MA) for products less than 1 kb and Pfu Ultra II Hotstart (Agilent Technologies, Santa Clara, CA) for products greater than 1 kb. All restriction enzymes were obtained from New England Biolabs. Plasmids were prepared from E. coli using Econospin columns (Epoch Life Science, Missouri City, TX) according to manufacturer’s instructions. Sequencing was performed by Elim Biopharmaceuticals, Inc (Hayward, CA).
The plasmid pCS2304 was constructed by PCR amplifying a DNA fragment encoding a CMV promoter, d2EGFP coding sequence, the BGH-polyA tail, using the primers BglII CMV Fwd (5′-AATAAGATCTTAGTTATTAATAGTAATCAATTACGGGGTCATTAGT-3′) and Mfe bgh Rev (5′-TATTCAATTGTTTGCTGTTTCGTCCTCCG-3′) and cloning the fragment between the BglII and MfeI restriction sites in pcDNA5/FRT. Spacer regions of different lengths were PCR amplified from the LacZ gene using the primers BstBi-Xma-mid LacZ Fwd: (5′-ATATTCGAAAATACCCGGGA TACTGTCGTCGTCCCCT-3′) and 500 bp LacZ rev (5′-AATAGGTACCATCGATAAT AACCGGTGTGTAGATGGGCGCATCG-3′) for the 500 bp spacer, 1000 bp LacZ rev (5′-AATAGGTACCATCGATAATAACCGGTTTG GCCCTGTCAGAAATCCAGGGG-3′) for the 1000 bp spacer, and 2500 bp LacZ rev (5′-AA TAGGTACCATCGATAATAACCGGTAAGCGCTGTCACCGGAATCAA-3′) for the 2500 bp spacer. These fragments were subsequently digested with BstBI and BamHI and cloned between the BstBI and BamHI restriction sites in the above intermediate plasmid to create pCS2722 (Additional file 1: Figure S5). The Rev primers used for amplifying the spacer incorporated AgeI and ClaI restriction sites on the 3′ end of the spacer, which were used for miRNA cloning in downstream steps. The resulting plasmids include two expression cassettes, one that drives the expression of d2EGFP and one that drives expression of different spacers that include restrictions sites on the 5′ end (BstBI, XmaI) and 3′ end (AgeI, ClaI) and is the base plasmid which was used in all OFF control switch studies in this work. The BstBI and XmaI restriction sites were used to clone the ribozyme switches and associated controls, and the AgeI and ClaI restriction sites were used to clone the miRNAs and associated controls. Finally, the plasmid contains FRT recombinase sites for making stable cell lines with the FLP recombinase system (Life Technologies).
We constructed pCS2934 (Additional file 1: Figure S5), which encodes MS2-DsRed expression under the control of an inducible promoter. A DNA fragment encoding CMV-TetO2-MS2-DsRed was obtained by digesting pCS2892  with BglII and AvrII, and purifying the 1800 bp fragment by gel extraction. The backbone pCS2722 was digested with BglII and AvrII and the two DNA fragments were ligated together to form pCS2934, which includes an expression cassette encoding the expression of an OFF control device downstream of a cassette which encodes the expression of the MS2-DsRed fusion gene driven by the CMV-TetO2 promoter. A detailed list of all plasmids used in this work is contained in Additional file 1: Table S2.
For the construction of all OFF control devices, the miRNAs were cloned downstream of the spacer region using the AgeI and ClaI restriction sites. The ribozyme switches and controls were cloned upstream of the spacer region using the BstBI and XmaI restriction sites. All miRNA and ribozyme components were constructed by ordering two ultramer oligonucleotides (Integrated DNA Technologies), which were reverse complements of each other at their 3′ ends. The oligonucleotide pairs were subjected to five rounds of annealing and extension using Taq polymerase (New England Biolabs) in a thermocycler. The resulting DNA fragments were subsequently digested and cloned. The sequences of all miRNAs and ribozymes used in this study are presented in Additional file 1: Table S3.
Mammalian cell culture
HEK293 Flp-In T-REx cells (Life Technologies) were maintained in DMEM supplemented with 10% FBS at 37°C in a 5% CO2-humidified incubator. Stable transfection of HEK293 Flp-In T-REx cell lines was performed using the Flp-In recombinase system (Life Technologies) according to the manufacturer’s instructions to generate isogenic stable cell lines. Stable integrants were selected using 200 μg/ml hygromycin B (Life Technologies), whereas stable cell lines were maintained in 100 μg/ml hygromycin B.
Measuring fluorescent reporter expression through flow cytometry assays
miRNA induction experiments were performed by first seeding 24-well cell culture plates with stable cell lines at 2.5×105 cells per well. After 48 hours, cells were trypsinized and subjected to flow cytometry analysis on a Cell Lab Quanta SC MPL (Beckman Coulter, Brea, CA), and the resulting data were analyzed using the FlowJo software (Tree Star, Ashland, OR). For experiments utilizing doxycycline induction, fresh media containing 0, 0.05, 0.2, 0.8, 3.2, or 50 ng/mL doxycycline was added to the wells 24 hours after seeding and measured by flow cytometry as described above. Cells were initially gated for viability by electronic volume and side scatter. GFP and DsRed fluorescence of viable cells were measured through 525- and 670-nm bandpass filters, respectively, after excitation with a 488-nm laser. The levels of the fluorescent reporters were calculated as the median fluorescence of the stable cell population (Additional file 1: Figure S6). All fluorescent reporter levels were normalized to the corresponding fluorescent reporter level from cells harboring a control construct lacking a miRNA under the same conditions. At least two biological replicates were included in each experiment, and reported data are the averages of at least three independent experiments. Error bars represent ±1 standard deviation.
Measuring miRNA levels through Taqman assays
The levels of miRNAs in the cells were quantified using custom Taqman small RNA assays (Applied Biosystems, Carlsbad, CA) according to the manufacturer’s instructions. Cells at different experimental conditions were plated in duplicate in 96-well tissue culture plates at a concentration of 10,000 cells per well. After 48 hours, the cells in one well were trypsinized and counted in a hemocytometer to obtain an accurate count of the number of cells per well for each experimental condition. The cells in the remaining well were lysed by adding 50 μl of Taqman lysis buffer and incubating for 8 minutes. The lysis reaction was stopped with the addition of 5 μl of stop buffer, resulting in a total volume of 55 μl of lysate per sample. A fixed volume of 5 μl of this lysate was used as input into the reverse transcription (RT) reaction.
Input RNA was reverse transcribed using the TaqMan miRNA Reverse Transcription Kit and custom miRNA-specific stem-loop primers (Applied BioSystems) in a small-scale RT reaction (1.387 μl of H2O, 0.5 μl of 10X reverse transcription buffer, 0.063 μl of RNase inhibitor (20 units/μl), 0.05 μl of 100 mM dNTPs with dTTP, 0.33 μl of multiscribe reverse transcriptase, 1.67 μl of input RNA; components other than the input RNA were prepared as a larger volume master mix). The reaction was run on an iCycler iQ system (BioRAD, Hercules, CA) at 16°C for 30 min, 42°C for 30 min, and 85°C for 5 min. The RNU 6B Taqman miRNA control was used to normalize the Ct values of the experimental miRNAs and to confirm the cell quantities measured by hemocytometer counting. Real-time PCR was carried out on an iCycler iQ thermocycler (BioRAD) at 95°C for 10 minutes, followed by 40 cycles of 95°C for 15 seconds and 60°C for 1 minute. The Ct was calculated using available RT-PCR Miner software , which automatically detects Ct and efficiency values for each PCR reaction. The mean of the resulting Ct values for the target gene of each sample were subtracted from the mean Ct value for the control gene. The resulting values were then converted from log2 to linear scale and normalized to the value from the sample lacking any miRNA with the same concentration of ligand.
Measuring binding properties between ribozyme switches and protein ligands
The binding properties between the protein-responsive ribozyme switches or RNA aptamers and the corresponding protein ligands (i.e., MS2, E2F1) were determined according to previously described methods . Briefly, ribozyme switches, aptamers, and appropriate controls were transcribed from DNA templates using the MEGAshortscript T7 kit (Life Technologies) and purification of the transcription products was performed using the RNA Clean & Concentrator Kit (Zymo Research) according to the manufacturers’ instructions. In all experiments, the HBS-N running buffer (GE Healthcare) (10 mM HEPES, 150 mM NaCl, pH 7.4) was used with no MgCl2 present, to prevent cleavage of the ribozyme. A dilution series of the target was prepared in running buffer using a minimum of five concentrations for kinetic analysis. For each sample concentration, the ribozyme switch was captured onto the sample flow cell (FC2) for 30 seconds at a flow rate of 10 μL/minute, the target solution was injected over both flow cells at a flow rate of 30 μL/minute to monitor target association, and running buffer was injected over both flow cells at a flow rate of 30 μL/minute to monitor target dissociation.
Data processing and analysis were performed using Biacore X100 Evaluation Software version 2.0 (GE Healthcare, San Francisco, CA). A double-referencing method was performed to process all datasets [42,43]. Data from the sample flow cell (FC2) were referenced first by subtracting data from the reference flow cell (FC1) to correct for bulk refractive index changes, nonspecific binding, injection noise, matrix effects, and baseline drift. The reference-subtracted data (FC2-FC1) were double-referenced with a blank injection of running buffer to account for any systematic drift over the course of the injection. The double-referenced data were fit to a 1:1 binding model for kinetic analysis to calculate the k a and k d between the ribozyme and the protein. The overall dissociation constant, K D, was calculated from k a , and k d .
Gel-based ribozyme cleavage assays
Gel-based ribozyme cleavage assays were performed as previously described . Briefly, all gel-based ribozyme cleavage assays were performed in a physiologically relevant reaction buffer (50 μl) composed of 500 μM MgCl2, 100 mM NaCl, and 50 mM Tris–HCl (pH 7.5) at 37°C. A 12-nt RNA blocking sequence that is complementary to part of the ribozyme transcript was inserted at the 5′ end of the sTRSV HHRz transcript to generate a cis-blocked ribozyme construct. In the reaction volume, 75 nM of full-length RNA was first incubated with 2.5 μM DNA activator strand (5′-AAACAACTTTGTTTGTTTCCCCC-3′), which is ordered from Integrated DNA Technologies as an oligonucleotide with standard desalting, for 2 min to activate the blocked RNA. A zero time-point aliquot was taken before initiating the self-cleavage reaction with the addition of MgCl2 and the indicated concentration of input protein. Reactions were quenched after 20 min with addition of 3 volumes of RNA stop/load buffer (95% formamide, 30 mM EDTA, 0.25% bromophenol blue, 0.25% xylene cyanol) and placed on ice. Samples were heated to 95°C for 5 min, snap cooled on ice for 5 min, and size-fractionated on a denaturing (8.3 M urea) 10% polyacrylamide gel at 25 W for 45–60 min. Gels were exposed overnight on a phosphor screen and imaged on a FX Molecular Imager (BioRAD). The relative levels of the full-length transcript and cleaved products were determined by phosphorimaging analysis.
We thank M. Mathur, M. Siddiqui for valuable comments on the transcript. This work was supported by the Defense Advanced Research Projects Agency [HR0011-11-2-0002 to CDS] the National Science Foundation [fellowship to RJB], and ARCS [fellowship to RJB]. RJB designed research, performed research, and wrote the article; SW designed the E2F1-responsive ribozyme switch; CDS designed research and wrote the article.
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