Self organization of the kinesin-microtubule system was implemented as a novel template to create percolated nanofiber networks. Asters of microtubule seeds were immobilized on glass surfaces and their growth ...
Authors: Vivek Verma, Jeffrey M Catchmark, Nicole R Brown and William O Hancock
Citation:Journal of Biological Engineering
2013
6:23
Stink bugs represent a major agricultural pest complex attacking more than 200 wild and cultivated plants, including cotton in the southeastern US. Stink bug feeding on developing cotton bolls will cause boll ...
Authors: Adnan Mustafic, Erin E Roberts, Michael D Toews and Mark A Haidekker
Citation:Journal of Biological Engineering
2013
7:5
Among the important principles in biology that should be taught in biological engineering educational programs is the principle of optimization, what it means, why it is important, and how it comes about. This...
Authors: Arthur T Johnson
Citation:Journal of Biological Engineering
2013
7:6
Like other bacteria, Escherichia coli must carefully regulate the intracellular concentration of sodium ion (Na+). During the bacterial production of any organic acid, cations like Na+ invariably accumulate durin...
Authors: Xianghao Wu, Ronni Altman, Mark A Eiteman and Elliot Altman
Citation:Journal of Biological Engineering
2013
7:3
There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assembl...
Authors: Mariana Leguia, Jennifer AN Brophy, Douglas Densmore, Angel Asante and J Christopher Anderson
Citation:Journal of Biological Engineering
2013
7:2
Sweet sorghum is a domesticated grass containing a sugar-rich juice that can be readily utilized for ethanol production. Most of the sugar is stored inside the cells of the stalk tissue and can be difficult to...
Authors: Fei Jia, Jeerwan Chawhuaymak, Mark R Riley, Werner Zimmt and Kimberly L Ogden
Citation:Journal of Biological Engineering
2013
7:1
Conventional diagnosis and identification of bacteria requires shipment of samples to a laboratory for genetic and biochemical analysis. This process can take days and imposes significant delay to action in si...
Authors: Euiwon Bae, Dawei Ying, Donald Kramer, Valery Patsekin, Bartek Rajwa, Cheryl Holdman, Jennifer Sturgis, V Jo Davisson and J Paul Robinson
Citation:Journal of Biological Engineering
2012
6:12
Tumor shape and size effect on drug delivery to solid tumors are studied, based on the application of the governing equations for fluid flow, i.e., the conservation laws for mass and momentum, to physiological...
Authors: M Soltani and Pu Chen
Citation:Journal of Biological Engineering
2012
6:4
Recombinant protein production is a process of great industrial interest, with products that range from pharmaceuticals to biofuels. Since high level production of recombinant protein imposes significant stres...
Authors: Martin Dragosits, Daniel Nicklas and Ilias Tagkopoulos
Citation:Journal of Biological Engineering
2012
6:2
Gene delivery approaches serve as a platform to modify gene expression of a cell population with applications including functional genomics, tissue engineering, and gene therapy. The delivery of exogenous gene...
Authors: Tadas Kasputis and Angela K Pannier
Citation:Journal of Biological Engineering
2012
6:17
A computer numerical control (CNC) apparatus was used to perform droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling on a single superhydrophobic surface and a multi-chambered PCB h...
Authors: David J You and Jeong-Yeol Yoon
Citation:Journal of Biological Engineering
2012
6:15
In prior work, adding a gene to phage T7 that degraded the host K1 capsule facilitated growth when plated on capsulated hosts. However, the transgenic protein (an endosialidase) is expressed as an exoenzyme, r...
Authors: Eric G Gladstone, Ian J Molineux and James J Bull
Citation:Journal of Biological Engineering
2012
6:13
The Respiration Activity Monitoring System (RAMOS) is an established device to measure on-line the oxygen transfer rate (OTR), thereby, yielding relevant information about metabolic activities of microorganism...
Authors: Sven Hansen, Ioanna Hariskos, Bettina Luchterhand and Jochen Büchs
Citation:Journal of Biological Engineering
2012
6:11
Bistability is a fundamental property in engineered and natural systems, conferring the ability to switch and retain states. Synthetic bistable switches in prokaryotes have mainly utilized transcriptional comp...
Authors: Daniel Huang, William J Holtz and Michel M Maharbiz
Citation:Journal of Biological Engineering
2012
6:9
Controlling gene expression via small interfering RNA (siRNA) has opened the doors to a plethora of therapeutic possibilities, with many currently in the pipelines of drug development for various ocular diseas...
Authors: Ajit Thakur, Scott Fitzpatrick, Abeyat Zaman, Kapilan Kugathasan, Ben Muirhead, Gonzalo Hortelano and Heather Sheardown
Citation:Journal of Biological Engineering
2012
6:7
Evolutionary dynamics of microbial organisms can now be visualized using the Visualizing Evolution in Real Time (VERT) system, in which several isogenic strains expressing different fluorescent proteins compet...
Authors: James Winkler and Katy C Kao
Citation:Journal of Biological Engineering
2012
6:3
Biological Computer Aided Design (bioCAD) assists the de novo design and selection of existing genetic components to achieve a desired biological activity, as part of an integrated design-build-test cycle. To mee...
Authors: Joanna Chen, Douglas Densmore, Timothy S Ham, Jay D Keasling and Nathan J Hillson
Citation:Journal of Biological Engineering
2012
6:1
Traumatic spinal cord injury ( SCI) leads to serious neurological and functional deficits through a chain of pathophysiological events. At the molecular level, progressive damage is initially revealed by collapse...
Authors: Bojun Chen, Mahvash Zuberi, Richard Ben Borgens and Youngnam Cho
Citation:Journal of Biological Engineering
2012
6:18
Fluorescent reporter proteins have revolutionized our understanding of cellular bioprocesses by enabling live cell imaging with exquisite spatio-temporal resolution. Existing fluorescent proteins are predomina...
Authors: Arnab Mukherjee, Kevin B Weyant, Joshua Walker and Charles M Schroeder
Citation:Journal of Biological Engineering
2012
6:20
Evolution has selected for organisms that benefit from genetically encoded cell-cell communication. Engineers have begun to repurpose elements of natural communication systems to realize programmed pattern for...
Authors: Monica E Ortiz and Drew Endy
Citation:Journal of Biological Engineering
2012
6:16
Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making p...
Authors: Patrick M Boyle, Devin R Burrill, Mara C Inniss, Christina M Agapakis, Aaron Deardon, Jonathan G DeWerd, Michael A Gedeon, Jacqueline Y Quinn, Morgan L Paull, Anugraha M Raman, Mark R Theilmann, Lu Wang, Julia C Winn, Oliver Medvedik, Kurt Schellenberg, Karmella A Haynes…
Citation:Journal of Biological Engineering
2012
6:8
AC Biosusceptometry (ACB) was previously employed towards recording gastrointestinal motility. Our data show a reliable and successful evaluation of gastrointestinal transit of liquid and solid meals in rats, ...
Authors: Caio C Quini, Madileine F Américo, Luciana A Corá, Marcos FF Calabresi, Matheus Alvarez, Ricardo B Oliveira and JoseRicardo A Miranda
Citation:Journal of Biological Engineering
2012
6:6
Biohydrogen from cyanobacteria has attracted public interest due to its potential as a renewable energy carrier produced from solar energy and water. Anabaena siamensis TISTR 8012, a novel strain isolated from ri...
Authors: Wanthanee Khetkorn, Peter Lindblad and Aran Incharoensakdi
Citation:Journal of Biological Engineering
2012
6:19
Modeling various neuronal functions in search of emergent properties may achieve success when the gold standard of replicating the models in physical systems starts exhibiting some of these properties. Since v...
Authors: Kunjumon I Vadakkan
Citation:Journal of Biological Engineering
2012
6:21
Temperature-sensitive (Ts) plasmids are useful tools for genetic engineering, but there are currently none compatible with the gram positive, thermophilic, obligate anaerobe, Clostridium thermocellum. Traditional...
Authors: Daniel G Olson and Lee R Lynd
Citation:Journal of Biological Engineering
2012
6:5
Rapid increase in multi-walled carbon nanotube (MWCNT) production for their industrial and biomedical applications has led to concerns over the effects of MWCNTs on human health and the environment. Both anima...
Authors: Hemang Patel and Soonjo Kwon
Citation:Journal of Biological Engineering
2012
6:22
Synthetic biology is an emerging field that combines molecular biology with engineering principles, which requires abstraction levels applied to a modular biological componentry. The Registry of Standard Biolo...
Authors: Matthijn C Hesselman, Jasper J Koehorst, Thijs Slijkhuis, Dorett I Odoni, Floor Hugenholtz and MarkW J van Passel
Citation:Journal of Biological Engineering
2012
6:14
Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthe...
Authors: Oliver Purcell, Claire S Grierson, Mario di Bernardo and Nigel J Savery
Citation:Journal of Biological Engineering
2012
6:10
A modified BioBrick™ assembly method was developed with higher fidelity than current protocols. The method utilizes a PCR reaction with a standard primer set to amplify the inserted part. Background colonies a...
Authors: Michael A Speer and Tom L Richard
Citation:Journal of Biological Engineering
2011
5:17
In a globalized word, prevention of infectious diseases is a major challenge. Rapid detection of viable virus particles in water and other environmental samples is essential to public health risk assessment, h...
Authors: Felipe T Lee-Montiel, Kelly A Reynolds and Mark R Riley
Citation:Journal of Biological Engineering
2011
5:16
CD-ELISA uses the microfluidic ranking method and centrifugal force to control the testing solution as it flows into the reaction region. The most challenging part of CD-ELISA is controlling the flow process f...
Authors: Samuel I En Lin
Citation:Journal of Biological Engineering
2011
5:15
On February 15, 2008, the National Academy of Engineering unveiled their list of 14 Grand Challenges for Engineering. Building off of tremendous advancements in the past century, these challenges were selected...
Authors: Ben J Stuart
Citation:Journal of Biological Engineering
2011
5:14
The threatened plant Centella asiatica L. is traditionallyused for a number of remedies. In vitro plant propagation and enhanced metabolite production of active metabolites through biotechnological approaches has...
Authors: Yamin Bibi, Muhammad Zia, Sobia Nisa, Darima Habib, Abdul Waheed and Fayyaz M Chaudhary
Citation:Journal of Biological Engineering
2011
5:13
As engineered biological systems become more complex, it is increasingly common to express multiple operons from different plasmids and inducible expression systems within a single host cell. Optimizing such s...
Authors: Taek Soon Lee, Rachel A Krupa, Fuzhong Zhang, Meghdad Hajimorad, William J Holtz, Nilu Prasad, Sung Kuk Lee and Jay D Keasling
Citation:Journal of Biological Engineering
2011
5:12
Stink bugs (Hemiptera: Pentatomidae) comprise a critically important insect pest complex affecting 12 major crops worldwide including cotton. In the US, stink bug damage to developing cotton bolls causes boll ...
Authors: Jinjun Xia, Adnan Mustafic, Michael D Toews and Mark A Haidekker
Citation:Journal of Biological Engineering
2011
5:11
Engineers frequently vary design parameters to optimize the behaviour of a system. However, synthetic biologists lack the tools to rapidly explore a critical design parameter, gene expression level, and have n...
Authors: Joshua T Kittleson, Sherine Cheung and JChristopher Anderson
Citation:Journal of Biological Engineering
2011
5:10
Members of the synthetic biology community have discussed the significance of word selection when describing synthetic biology to the general public. In particular, many leaders proposed the word "create" was ...
Authors: Brianna Pearson, Sam Snell, Kyri Bye-Nagel, Scott Tonidandel, Laurie J Heyer and A Malcolm Campbell
Citation:Journal of Biological Engineering
2011
5:9
Bacterial cell lysis is a widely studied mechanism that can be achieved through the intracellular expression of phage native lytic proteins. This mechanism can be exploited for programmed cell death and for ge...
Authors: Lorenzo Pasotti, Susanna Zucca, Manuel Lupotto, Maria Gabriella Cusella De Angelis and Paolo Magni
Citation:Journal of Biological Engineering
2011
5:8
FeFe-hydrogenases are the most active class of H2-producing enzymes known in nature and may have important applications in clean H2 energy production. Many potential uses are currently complicated by a crucial we...
Authors: Buz Barstow, Christina M Agapakis, Patrick M Boyle, Gerald Grandl, Pamela A Silver and Edwin H Wintermute
Citation:Journal of Biological Engineering
2011
5:7
Microfluidics holds great promise to revolutionize various areas of biological engineering, such as single cell analysis, environmental monitoring, regenerative medicine, and point-of-care diagnostics. Despite...
Authors: Mandy LY Sin, Jian Gao, Joseph C Liao and Pak Kin Wong
Citation:Journal of Biological Engineering
2011
5:6
Two current technologies used in biosensor development are very promising: 1. The sol-gel process of making microporous glass at room temperature, and 2. Using a fluorescent compound that undergoes fluorescenc...
Authors: Michael F Yoder and William S Kisaalita
Citation:Journal of Biological Engineering
2011
5:4
An investigation of the electrochemical activity of human white blood cells (WBC) for biofuel cell (BFC) applications is described. WBCs isolated from whole human blood were suspended in PBS and introduced int...
Authors: Gusphyl A Justin, Yingze Zhang, X Tracy Cui, Charles W Bradberry, Mingui Sun and Robert J Sclabassi
Citation:Journal of Biological Engineering
2011
5:5
The ability to compose biological systems from smaller elements that act independently of the other upon assembly may help make the forward engineering of biological systems practical. Engineering biology in t...
Authors: Meghdad Hajimorad, Paul R Gray and Jay D Keasling
Citation:Journal of Biological Engineering
2011
5:3
The U.S. National Academy of Engineering (NAE) recently published a document presenting "Grand Challenges for Engineering". This list was proposed by leading engineers and scientists from around the world at t...
Authors: Mark R Riley, Charles P Gerba and Menachem Elimelech
Citation:Journal of Biological Engineering
2011
5:2
Dendritic cells (DCs) are antigen-presenting immune cells that interact with T cells and have been widely studied for vaccine applications. To achieve this, DCs can be manipulated by lentiviral vectors (LVs) t...
Authors: April Tai, Steven Froelich, Kye-Il Joo and Pin Wang
Citation:Journal of Biological Engineering
2011
5:1
BioBrick standard biological parts are designed to make biological systems easier to engineer (e.g. assemble, manipulate, and modify). There are over 5,000 parts available in the Registry of Standard Biologica...
Authors: Julie E Norville, Ratmir Derda, Saurabh Gupta, Kelly A Drinkwater, Angela M Belcher, Andres E Leschziner and Thomas F Knight Jr
Citation:Journal of Biological Engineering
2010
4:17
In synthetic biology, gene regulatory circuits are often constructed by combining smaller circuit components. Connections between components are achieved by transcription factors acting on promoters. If the in...
Authors: Kyung H Kim and Herbert M Sauro
Citation:Journal of Biological Engineering
2010
4:16
For years, the field of drug delivery has focused on (1) controlling the release of a therapeutic and (2) targeting the therapeutic to a specific cell type. These research endeavors have concentrated mainly on...
Authors: Jin-Oh You, Dariela Almeda, George JC Ye and Debra T Auguste
Citation:Journal of Biological Engineering
2010
4:15
Most of synthetic circuits developed so far have been designed by an ad hoc approach, using a small number of components (i.e. LacI, TetR) and a trial and error strategy. We are at the point where an increasin...
Authors: Francesca Ceroni, Simone Furini, Emanuele Giordano and Silvio Cavalcanti
Citation:Journal of Biological Engineering
2010
4:14
Published descriptions of biology protocols are often ambiguous and incomplete, making them difficult to replicate in other laboratories. However, there is increasing benefit to formalizing the descriptions of...
Authors: Vaishnavi Ananthanarayanan and William Thies
Citation:Journal of Biological Engineering
2010
4:13