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  1. Content type: Research

    Self organization of the kinesin-microtubule system was implemented as a novel template to create percolated nanofiber networks. Asters of microtubule seeds were immobilized on glass surfaces and their growth ...

    Authors: Vivek Verma, Jeffrey M Catchmark, Nicole R Brown and William O Hancock

    Citation: Journal of Biological Engineering 2013 6:23

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  2. Content type: Research

    Stink bugs represent a major agricultural pest complex attacking more than 200 wild and cultivated plants, including cotton in the southeastern US. Stink bug feeding on developing cotton bolls will cause boll ...

    Authors: Adnan Mustafic, Erin E Roberts, Michael D Toews and Mark A Haidekker

    Citation: Journal of Biological Engineering 2013 7:5

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  3. Content type: Review

    Among the important principles in biology that should be taught in biological engineering educational programs is the principle of optimization, what it means, why it is important, and how it comes about. This...

    Authors: Arthur T Johnson

    Citation: Journal of Biological Engineering 2013 7:6

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  4. Content type: Research

    Like other bacteria, Escherichia coli must carefully regulate the intracellular concentration of sodium ion (Na+). During the bacterial production of any organic acid, cations like Na+ invariably accumulate durin...

    Authors: Xianghao Wu, Ronni Altman, Mark A Eiteman and Elliot Altman

    Citation: Journal of Biological Engineering 2013 7:3

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  5. Content type: Methodology

    There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assembl...

    Authors: Mariana Leguia, Jennifer AN Brophy, Douglas Densmore, Angel Asante and J Christopher Anderson

    Citation: Journal of Biological Engineering 2013 7:2

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  6. Content type: Research

    Sweet sorghum is a domesticated grass containing a sugar-rich juice that can be readily utilized for ethanol production. Most of the sugar is stored inside the cells of the stalk tissue and can be difficult to...

    Authors: Fei Jia, Jeerwan Chawhuaymak, Mark R Riley, Werner Zimmt and Kimberly L Ogden

    Citation: Journal of Biological Engineering 2013 7:1

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  7. Content type: Research

    Conventional diagnosis and identification of bacteria requires shipment of samples to a laboratory for genetic and biochemical analysis. This process can take days and imposes significant delay to action in si...

    Authors: Euiwon Bae, Dawei Ying, Donald Kramer, Valery Patsekin, Bartek Rajwa, Cheryl Holdman, Jennifer Sturgis, V Jo Davisson and J Paul Robinson

    Citation: Journal of Biological Engineering 2012 6:12

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  9. Content type: Research

    Recombinant protein production is a process of great industrial interest, with products that range from pharmaceuticals to biofuels. Since high level production of recombinant protein imposes significant stres...

    Authors: Martin Dragosits, Daniel Nicklas and Ilias Tagkopoulos

    Citation: Journal of Biological Engineering 2012 6:2

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  11. Content type: Methodology

    A computer numerical control (CNC) apparatus was used to perform droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling on a single superhydrophobic surface and a multi-chambered PCB h...

    Authors: David J You and Jeong-Yeol Yoon

    Citation: Journal of Biological Engineering 2012 6:15

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  12. Content type: Research

    In prior work, adding a gene to phage T7 that degraded the host K1 capsule facilitated growth when plated on capsulated hosts. However, the transgenic protein (an endosialidase) is expressed as an exoenzyme, r...

    Authors: Eric G Gladstone, Ian J Molineux and James J Bull

    Citation: Journal of Biological Engineering 2012 6:13

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  13. Content type: Methodology

    The Respiration Activity Monitoring System (RAMOS) is an established device to measure on-line the oxygen transfer rate (OTR), thereby, yielding relevant information about metabolic activities of microorganism...

    Authors: Sven Hansen, Ioanna Hariskos, Bettina Luchterhand and Jochen Büchs

    Citation: Journal of Biological Engineering 2012 6:11

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  14. Content type: Research

    Bistability is a fundamental property in engineered and natural systems, conferring the ability to switch and retain states. Synthetic bistable switches in prokaryotes have mainly utilized transcriptional comp...

    Authors: Daniel Huang, William J Holtz and Michel M Maharbiz

    Citation: Journal of Biological Engineering 2012 6:9

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  15. Content type: Review

    Controlling gene expression via small interfering RNA (siRNA) has opened the doors to a plethora of therapeutic possibilities, with many currently in the pipelines of drug development for various ocular diseas...

    Authors: Ajit Thakur, Scott Fitzpatrick, Abeyat Zaman, Kapilan Kugathasan, Ben Muirhead, Gonzalo Hortelano and Heather Sheardown

    Citation: Journal of Biological Engineering 2012 6:7

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  17. Content type: Research

    Biological Computer Aided Design (bioCAD) assists the de novo design and selection of existing genetic components to achieve a desired biological activity, as part of an integrated design-build-test cycle. To mee...

    Authors: Joanna Chen, Douglas Densmore, Timothy S Ham, Jay D Keasling and Nathan J Hillson

    Citation: Journal of Biological Engineering 2012 6:1

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  18. Content type: Research

    Traumatic spinal cord injury ( SCI) leads to serious neurological and functional deficits through a chain of pathophysiological events. At the molecular level, progressive damage is initially revealed by collapse...

    Authors: Bojun Chen, Mahvash Zuberi, Richard Ben Borgens and Youngnam Cho

    Citation: Journal of Biological Engineering 2012 6:18

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  19. Content type: Research

    Fluorescent reporter proteins have revolutionized our understanding of cellular bioprocesses by enabling live cell imaging with exquisite spatio-temporal resolution. Existing fluorescent proteins are predomina...

    Authors: Arnab Mukherjee, Kevin B Weyant, Joshua Walker and Charles M Schroeder

    Citation: Journal of Biological Engineering 2012 6:20

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  20. Content type: Research

    Evolution has selected for organisms that benefit from genetically encoded cell-cell communication. Engineers have begun to repurpose elements of natural communication systems to realize programmed pattern for...

    Authors: Monica E Ortiz and Drew Endy

    Citation: Journal of Biological Engineering 2012 6:16

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  21. Content type: Methodology

    Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making p...

    Authors: Patrick M Boyle, Devin R Burrill, Mara C Inniss, Christina M Agapakis, Aaron Deardon, Jonathan G DeWerd, Michael A Gedeon, Jacqueline Y Quinn, Morgan L Paull, Anugraha M Raman, Mark R Theilmann, Lu Wang, Julia C Winn, Oliver Medvedik, Kurt Schellenberg, Karmella A Haynes…

    Citation: Journal of Biological Engineering 2012 6:8

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  22. Content type: Methodology

    AC Biosusceptometry (ACB) was previously employed towards recording gastrointestinal motility. Our data show a reliable and successful evaluation of gastrointestinal transit of liquid and solid meals in rats, ...

    Authors: Caio C Quini, Madileine F Américo, Luciana A Corá, Marcos FF Calabresi, Matheus Alvarez, Ricardo B Oliveira and JoseRicardo A Miranda

    Citation: Journal of Biological Engineering 2012 6:6

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  23. Content type: Research

    Biohydrogen from cyanobacteria has attracted public interest due to its potential as a renewable energy carrier produced from solar energy and water. Anabaena siamensis TISTR 8012, a novel strain isolated from ri...

    Authors: Wanthanee Khetkorn, Peter Lindblad and Aran Incharoensakdi

    Citation: Journal of Biological Engineering 2012 6:19

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  24. Content type: Letters to the Editor

    Modeling various neuronal functions in search of emergent properties may achieve success when the gold standard of replicating the models in physical systems starts exhibiting some of these properties. Since v...

    Authors: Kunjumon I Vadakkan

    Citation: Journal of Biological Engineering 2012 6:21

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  25. Content type: Research

    Temperature-sensitive (Ts) plasmids are useful tools for genetic engineering, but there are currently none compatible with the gram positive, thermophilic, obligate anaerobe, Clostridium thermocellum. Traditional...

    Authors: Daniel G Olson and Lee R Lynd

    Citation: Journal of Biological Engineering 2012 6:5

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  27. Content type: Research

    Synthetic biology is an emerging field that combines molecular biology with engineering principles, which requires abstraction levels applied to a modular biological componentry. The Registry of Standard Biolo...

    Authors: Matthijn C Hesselman, Jasper J Koehorst, Thijs Slijkhuis, Dorett I Odoni, Floor Hugenholtz and MarkW J van Passel

    Citation: Journal of Biological Engineering 2012 6:14

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  28. Content type: Letters to the Editor

    Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthe...

    Authors: Oliver Purcell, Claire S Grierson, Mario di Bernardo and Nigel J Savery

    Citation: Journal of Biological Engineering 2012 6:10

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  30. Content type: Research

    In a globalized word, prevention of infectious diseases is a major challenge. Rapid detection of viable virus particles in water and other environmental samples is essential to public health risk assessment, h...

    Authors: Felipe T Lee-Montiel, Kelly A Reynolds and Mark R Riley

    Citation: Journal of Biological Engineering 2011 5:16

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  33. Content type: Research

    The threatened plant Centella asiatica L. is traditionallyused for a number of remedies. In vitro plant propagation and enhanced metabolite production of active metabolites through biotechnological approaches has...

    Authors: Yamin Bibi, Muhammad Zia, Sobia Nisa, Darima Habib, Abdul Waheed and Fayyaz M Chaudhary

    Citation: Journal of Biological Engineering 2011 5:13

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  34. Content type: Methodology

    As engineered biological systems become more complex, it is increasingly common to express multiple operons from different plasmids and inducible expression systems within a single host cell. Optimizing such s...

    Authors: Taek Soon Lee, Rachel A Krupa, Fuzhong Zhang, Meghdad Hajimorad, William J Holtz, Nilu Prasad, Sung Kuk Lee and Jay D Keasling

    Citation: Journal of Biological Engineering 2011 5:12

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  35. Content type: Research

    Stink bugs (Hemiptera: Pentatomidae) comprise a critically important insect pest complex affecting 12 major crops worldwide including cotton. In the US, stink bug damage to developing cotton bolls causes boll ...

    Authors: Jinjun Xia, Adnan Mustafic, Michael D Toews and Mark A Haidekker

    Citation: Journal of Biological Engineering 2011 5:11

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  36. Content type: Research

    Engineers frequently vary design parameters to optimize the behaviour of a system. However, synthetic biologists lack the tools to rapidly explore a critical design parameter, gene expression level, and have n...

    Authors: Joshua T Kittleson, Sherine Cheung and JChristopher Anderson

    Citation: Journal of Biological Engineering 2011 5:10

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  37. Content type: Letter to the editors

    Members of the synthetic biology community have discussed the significance of word selection when describing synthetic biology to the general public. In particular, many leaders proposed the word "create" was ...

    Authors: Brianna Pearson, Sam Snell, Kyri Bye-Nagel, Scott Tonidandel, Laurie J Heyer and A Malcolm Campbell

    Citation: Journal of Biological Engineering 2011 5:9

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  38. Content type: Research

    Bacterial cell lysis is a widely studied mechanism that can be achieved through the intracellular expression of phage native lytic proteins. This mechanism can be exploited for programmed cell death and for ge...

    Authors: Lorenzo Pasotti, Susanna Zucca, Manuel Lupotto, Maria Gabriella Cusella De Angelis and Paolo Magni

    Citation: Journal of Biological Engineering 2011 5:8

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  39. Content type: Research

    FeFe-hydrogenases are the most active class of H2-producing enzymes known in nature and may have important applications in clean H2 energy production. Many potential uses are currently complicated by a crucial we...

    Authors: Buz Barstow, Christina M Agapakis, Patrick M Boyle, Gerald Grandl, Pamela A Silver and Edwin H Wintermute

    Citation: Journal of Biological Engineering 2011 5:7

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  40. Content type: Review

    Microfluidics holds great promise to revolutionize various areas of biological engineering, such as single cell analysis, environmental monitoring, regenerative medicine, and point-of-care diagnostics. Despite...

    Authors: Mandy LY Sin, Jian Gao, Joseph C Liao and Pak Kin Wong

    Citation: Journal of Biological Engineering 2011 5:6

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  42. Content type: Research

    An investigation of the electrochemical activity of human white blood cells (WBC) for biofuel cell (BFC) applications is described. WBCs isolated from whole human blood were suspended in PBS and introduced int...

    Authors: Gusphyl A Justin, Yingze Zhang, X Tracy Cui, Charles W Bradberry, Mingui Sun and Robert J Sclabassi

    Citation: Journal of Biological Engineering 2011 5:5

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  44. Content type: Review

    The U.S. National Academy of Engineering (NAE) recently published a document presenting "Grand Challenges for Engineering". This list was proposed by leading engineers and scientists from around the world at t...

    Authors: Mark R Riley, Charles P Gerba and Menachem Elimelech

    Citation: Journal of Biological Engineering 2011 5:2

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  45. Content type: Research

    Dendritic cells (DCs) are antigen-presenting immune cells that interact with T cells and have been widely studied for vaccine applications. To achieve this, DCs can be manipulated by lentiviral vectors (LVs) t...

    Authors: April Tai, Steven Froelich, Kye-Il Joo and Pin Wang

    Citation: Journal of Biological Engineering 2011 5:1

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  46. Content type: Methodology

    BioBrick standard biological parts are designed to make biological systems easier to engineer (e.g. assemble, manipulate, and modify). There are over 5,000 parts available in the Registry of Standard Biologica...

    Authors: Julie E Norville, Ratmir Derda, Saurabh Gupta, Kelly A Drinkwater, Angela M Belcher, Andres E Leschziner and Thomas F Knight Jr

    Citation: Journal of Biological Engineering 2010 4:17

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  47. Content type: Research

    In synthetic biology, gene regulatory circuits are often constructed by combining smaller circuit components. Connections between components are achieved by transcription factors acting on promoters. If the in...

    Authors: Kyung H Kim and Herbert M Sauro

    Citation: Journal of Biological Engineering 2010 4:16

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  48. Content type: Review

    For years, the field of drug delivery has focused on (1) controlling the release of a therapeutic and (2) targeting the therapeutic to a specific cell type. These research endeavors have concentrated mainly on...

    Authors: Jin-Oh You, Dariela Almeda, George JC Ye and Debra T Auguste

    Citation: Journal of Biological Engineering 2010 4:15

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  49. Content type: Research

    Most of synthetic circuits developed so far have been designed by an ad hoc approach, using a small number of components (i.e. LacI, TetR) and a trial and error strategy. We are at the point where an increasin...

    Authors: Francesca Ceroni, Simone Furini, Emanuele Giordano and Silvio Cavalcanti

    Citation: Journal of Biological Engineering 2010 4:14

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  • ISSN: 1754-1611

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Journal of Biological Engineering

ISSN: 1754-1611