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  1. Content type: Research

    Conventional diagnosis and identification of bacteria requires shipment of samples to a laboratory for genetic and biochemical analysis. This process can take days and imposes significant delay to action in si...

    Authors: Euiwon Bae, Dawei Ying, Donald Kramer, Valery Patsekin, Bartek Rajwa, Cheryl Holdman, Jennifer Sturgis, V Jo Davisson and J Paul Robinson

    Citation: Journal of Biological Engineering 2012 6:12

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  2. Content type: Research

    Recombinant protein production is a process of great industrial interest, with products that range from pharmaceuticals to biofuels. Since high level production of recombinant protein imposes significant stres...

    Authors: Martin Dragosits, Daniel Nicklas and Ilias Tagkopoulos

    Citation: Journal of Biological Engineering 2012 6:2

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  3. Content type: Methodology

    A computer numerical control (CNC) apparatus was used to perform droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling on a single superhydrophobic surface and a multi-chambered PCB h...

    Authors: David J You and Jeong-Yeol Yoon

    Citation: Journal of Biological Engineering 2012 6:15

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  4. Content type: Research

    In prior work, adding a gene to phage T7 that degraded the host K1 capsule facilitated growth when plated on capsulated hosts. However, the transgenic protein (an endosialidase) is expressed as an exoenzyme, r...

    Authors: Eric G Gladstone, Ian J Molineux and James J Bull

    Citation: Journal of Biological Engineering 2012 6:13

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  5. Content type: Methodology

    The Respiration Activity Monitoring System (RAMOS) is an established device to measure on-line the oxygen transfer rate (OTR), thereby, yielding relevant information about metabolic activities of microorganism...

    Authors: Sven Hansen, Ioanna Hariskos, Bettina Luchterhand and Jochen Büchs

    Citation: Journal of Biological Engineering 2012 6:11

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  6. Content type: Research

    In a globalized word, prevention of infectious diseases is a major challenge. Rapid detection of viable virus particles in water and other environmental samples is essential to public health risk assessment, h...

    Authors: Felipe T Lee-Montiel, Kelly A Reynolds and Mark R Riley

    Citation: Journal of Biological Engineering 2011 5:16

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  7. Content type: Research

    The threatened plant Centella asiatica L. is traditionallyused for a number of remedies. In vitro plant propagation and enhanced metabolite production of active metabolites through biotechnological approaches has...

    Authors: Yamin Bibi, Muhammad Zia, Sobia Nisa, Darima Habib, Abdul Waheed and Fayyaz M Chaudhary

    Citation: Journal of Biological Engineering 2011 5:13

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  8. Content type: Methodology

    As engineered biological systems become more complex, it is increasingly common to express multiple operons from different plasmids and inducible expression systems within a single host cell. Optimizing such s...

    Authors: Taek Soon Lee, Rachel A Krupa, Fuzhong Zhang, Meghdad Hajimorad, William J Holtz, Nilu Prasad, Sung Kuk Lee and Jay D Keasling

    Citation: Journal of Biological Engineering 2011 5:12

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  9. Content type: Research

    Stink bugs (Hemiptera: Pentatomidae) comprise a critically important insect pest complex affecting 12 major crops worldwide including cotton. In the US, stink bug damage to developing cotton bolls causes boll ...

    Authors: Jinjun Xia, Adnan Mustafic, Michael D Toews and Mark A Haidekker

    Citation: Journal of Biological Engineering 2011 5:11

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  10. Content type: Research

    Engineers frequently vary design parameters to optimize the behaviour of a system. However, synthetic biologists lack the tools to rapidly explore a critical design parameter, gene expression level, and have n...

    Authors: Joshua T Kittleson, Sherine Cheung and JChristopher Anderson

    Citation: Journal of Biological Engineering 2011 5:10

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  11. Content type: Letter to the editors

    Members of the synthetic biology community have discussed the significance of word selection when describing synthetic biology to the general public. In particular, many leaders proposed the word "create" was ...

    Authors: Brianna Pearson, Sam Snell, Kyri Bye-Nagel, Scott Tonidandel, Laurie J Heyer and A Malcolm Campbell

    Citation: Journal of Biological Engineering 2011 5:9

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  12. Content type: Research

    Bacterial cell lysis is a widely studied mechanism that can be achieved through the intracellular expression of phage native lytic proteins. This mechanism can be exploited for programmed cell death and for ge...

    Authors: Lorenzo Pasotti, Susanna Zucca, Manuel Lupotto, Maria Gabriella Cusella De Angelis and Paolo Magni

    Citation: Journal of Biological Engineering 2011 5:8

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  13. Content type: Research

    FeFe-hydrogenases are the most active class of H2-producing enzymes known in nature and may have important applications in clean H2 energy production. Many potential uses are currently complicated by a crucial we...

    Authors: Buz Barstow, Christina M Agapakis, Patrick M Boyle, Gerald Grandl, Pamela A Silver and Edwin H Wintermute

    Citation: Journal of Biological Engineering 2011 5:7

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  14. Content type: Review

    Microfluidics holds great promise to revolutionize various areas of biological engineering, such as single cell analysis, environmental monitoring, regenerative medicine, and point-of-care diagnostics. Despite...

    Authors: Mandy LY Sin, Jian Gao, Joseph C Liao and Pak Kin Wong

    Citation: Journal of Biological Engineering 2011 5:6

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  15. Content type: Research

    An investigation of the electrochemical activity of human white blood cells (WBC) for biofuel cell (BFC) applications is described. WBCs isolated from whole human blood were suspended in PBS and introduced int...

    Authors: Gusphyl A Justin, Yingze Zhang, X Tracy Cui, Charles W Bradberry, Mingui Sun and Robert J Sclabassi

    Citation: Journal of Biological Engineering 2011 5:5

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  16. Content type: Review

    The U.S. National Academy of Engineering (NAE) recently published a document presenting "Grand Challenges for Engineering". This list was proposed by leading engineers and scientists from around the world at t...

    Authors: Mark R Riley, Charles P Gerba and Menachem Elimelech

    Citation: Journal of Biological Engineering 2011 5:2

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  17. Content type: Research

    Dendritic cells (DCs) are antigen-presenting immune cells that interact with T cells and have been widely studied for vaccine applications. To achieve this, DCs can be manipulated by lentiviral vectors (LVs) t...

    Authors: April Tai, Steven Froelich, Kye-Il Joo and Pin Wang

    Citation: Journal of Biological Engineering 2011 5:1

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  18. Content type: Methodology

    BioBrick standard biological parts are designed to make biological systems easier to engineer (e.g. assemble, manipulate, and modify). There are over 5,000 parts available in the Registry of Standard Biologica...

    Authors: Julie E Norville, Ratmir Derda, Saurabh Gupta, Kelly A Drinkwater, Angela M Belcher, Andres E Leschziner and Thomas F Knight Jr

    Citation: Journal of Biological Engineering 2010 4:17

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  19. Content type: Research

    In synthetic biology, gene regulatory circuits are often constructed by combining smaller circuit components. Connections between components are achieved by transcription factors acting on promoters. If the in...

    Authors: Kyung H Kim and Herbert M Sauro

    Citation: Journal of Biological Engineering 2010 4:16

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  20. Content type: Review

    For years, the field of drug delivery has focused on (1) controlling the release of a therapeutic and (2) targeting the therapeutic to a specific cell type. These research endeavors have concentrated mainly on...

    Authors: Jin-Oh You, Dariela Almeda, George JC Ye and Debra T Auguste

    Citation: Journal of Biological Engineering 2010 4:15

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  21. Content type: Research

    Most of synthetic circuits developed so far have been designed by an ad hoc approach, using a small number of components (i.e. LacI, TetR) and a trial and error strategy. We are at the point where an increasin...

    Authors: Francesca Ceroni, Simone Furini, Emanuele Giordano and Silvio Cavalcanti

    Citation: Journal of Biological Engineering 2010 4:14

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  22. Content type: Research

    One problem with engineered genetic circuits in synthetic microbes is their stability over evolutionary time in the absence of selective pressure. Since design of a selective environment for maintaining functi...

    Authors: Sean C Sleight, Bryan A Bartley, Jane A Lieviant and Herbert M Sauro

    Citation: Journal of Biological Engineering 2010 4:12

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  23. Content type: Review

    Molecular rotors are a group of fluorescent molecules that form twisted intramolecular charge transfer (TICT) states upon photoexcitation. When intramolecular twisting occurs, the molecular rotor returns to th...

    Authors: Mark A Haidekker and Emmanuel A Theodorakis

    Citation: Journal of Biological Engineering 2010 4:11

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  24. Content type: Research

    Membrane proteins are an important class of proteins, playing a key role in many biological processes, and are a promising target in pharmaceutical development. However, membrane proteins are often difficult t...

    Authors: Karan Bansal, Ke Yang, Goutam J Nistala, Robert B Gennis and Kaustubh D Bhalerao

    Citation: Journal of Biological Engineering 2010 4:6

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  25. Content type: Research

    A polarized light scattering technique was used to monitor the performance of a continuously operated foam fractionation process. The S11 and S12 parameters, elements of the light scattering matrix, combined toge...

    Authors: Janakiraman N Swamy, Czarena L Crofcheck and MP Mengüç

    Citation: Journal of Biological Engineering 2010 4:5

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  26. Content type: Research

    Positive feedback is a common mechanism used in the regulation of many gene circuits as it can amplify the response to inducers and also generate binary outputs and hysteresis. In the context of electrical cir...

    Authors: Goutam J Nistala, Kang Wu, Christopher V Rao and Kaustubh D Bhalerao

    Citation: Journal of Biological Engineering 2010 4:4

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  27. Content type: Research

    The engineering of metabolism holds tremendous promise for the production of desirable metabolites, particularly alternative fuels and other highly reduced molecules. Engineering approaches must redirect the t...

    Authors: Christina M Agapakis, Daniel C Ducat, Patrick M Boyle, Edwin H Wintermute, Jeffrey C Way and Pamela A Silver

    Citation: Journal of Biological Engineering 2010 4:3

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  28. Content type: Research

    The highly reactive aldehyde acrolein is a very potent endogenous toxin with a long half-life. Acrolein is produced within cells after insult, and is a central player in slow and progressive "secondary injury"...

    Authors: Youngnam Cho, Riyi Shi and Richard Ben Borgens

    Citation: Journal of Biological Engineering 2010 4:2

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  29. Content type: Methodology

    Standard biological parts, such as BioBricks™ parts, provide the foundation for a new engineering discipline that enables the design and construction of synthetic biological systems with a variety of applicati...

    Authors: JChristopher Anderson, John E Dueber, Mariana Leguia, Gabriel C Wu, Jonathan A Goler, Adam P Arkin and Jay D Keasling

    Citation: Journal of Biological Engineering 2010 4:1

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  30. Content type: Research

    Synthetic biology brings together concepts and techniques from engineering and biology. In this field, computer-aided design (CAD) is necessary in order to bridge the gap between computational modeling and bio...

    Authors: Deepak Chandran, Frank T Bergmann and Herbert M Sauro

    Citation: Journal of Biological Engineering 2009 3:19

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  31. Content type: Research

    Efflux pump activity has been associated with multidrug resistance phenotypes in bacteria, compromising the effectiveness of antimicrobial therapy. The development of methods for the early detection and quanti...

    Authors: Laura Paixão, Liliana Rodrigues, Isabel Couto, Marta Martins, Pedro Fernandes, Carla CCR de Carvalho, Gabriel A Monteiro, Filipe Sansonetty, Leonard Amaral and Miguel Viveiros

    Citation: Journal of Biological Engineering 2009 3:18

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  32. Content type: Research

    Cnidocysts isolated from cnidarian organisms are attractive as a drug-delivery platform due to their fast, efficient delivery of toxins. The cnidocyst could be utilized as the means to deliver therapeutics in ...

    Authors: Shawn C Oppegard, Peter A Anderson and David T Eddington

    Citation: Journal of Biological Engineering 2009 3:17

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  33. Content type: Research

    Biological engineering will play a significant role in solving many of the world's problems in medicine, agriculture, and the environment. Recently the U.S. National Academy of Engineering (NAE) released a doc...

    Authors: Jeong-Yeol Yoon and Mark R Riley

    Citation: Journal of Biological Engineering 2009 3:16

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  34. Content type: Research

    Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidop...

    Authors: Oxana Sorokina, Anita Kapus, Kata Terecskei, Laura E Dixon, Laszlo Kozma-Bognar, Ferenc Nagy and Andrew J Millar

    Citation: Journal of Biological Engineering 2009 3:15

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  35. Content type: Research

    Determination of lipophilicity as a tool for predicting pharmacokinetic molecular behavior is limited by the predictive power of available experimental models of the biomembrane. There is current interest, the...

    Authors: Sunday Olakunle Idowu, Morenikeji Ambali Adeyemo and Udochi Ihechiluru Ogbonna

    Citation: Journal of Biological Engineering 2009 3:14

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  36. Content type: Research

    Escherichia coli exhibits diauxic growth in sugar mixtures due to CRP-mediated catabolite repression and inducer exclusion related to phosphotransferase system enzyme activity. Replacement of the native crp gene ...

    Authors: Reza Khankal, Jonathan W Chin, Debashis Ghosh and Patrick C Cirino

    Citation: Journal of Biological Engineering 2009 3:13

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  37. Content type: Research

    Bacterial cellulose has been used in the food industry for applications such as low-calorie desserts, salads, and fabricated foods. It has also been used in the paper manufacturing industry to enhance paper st...

    Authors: Kuan-Chen Cheng, Jeff M Catchmark and Ali Demirci

    Citation: Journal of Biological Engineering 2009 3:12

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