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  1. Demineralized bone matrix (DBM), an allograft bone processed to better expose osteoinductive factors such as bone morphogenetic proteins (BMPs), is increasingly used for clinical bone repair. However, more ext...

    Authors: Soyon Kim, Jiabing Fan, Chung-Sung Lee, Chen Chen, Ksenia Bubukina and Min Lee
    Citation: Journal of Biological Engineering 2020 14:6
  2. An efficient biodegradation-strengthening approach was developed to improve penicillin G degradation from industrial bacterial residue in an expanded bed adsorption bioreactor (EBAB) is reported in this paper.

    Authors: Peng Wang, Chen Shen, Xiaochun Wang, Shouxin Liu, Luwei Li and Jinfeng Guo
    Citation: Journal of Biological Engineering 2020 14:5
  3. Knowledge of glutamate excitotoxicity has increased substantially over the past few decades, with multiple proposed pathways involved in inflicting damage. We sought to develop a monosodium glutamate (MSG) exp...

    Authors: Rick Liao, Thomas R. Wood and Elizabeth Nance
    Citation: Journal of Biological Engineering 2020 14:3
  4. Aptamers, single-stranded DNAs or RNAs, can be selected from a library containing random sequences using a method called Systematic Evolution of Ligands by EXponential Enrichment (SELEX). In SELEX, monitoring ...

    Authors: Qingxiu Liu, Wei Zhang, Siying Chen, Zhenjing Zhuang, Yi Zhang, Lingli Jiang and Jun Sheng LIN
    Citation: Journal of Biological Engineering 2020 14:1
  5. Controlling of biofilms formation in numerous pathogenic bacteria is one of the most difficult tasks in the control of bacterial diseases. Plasma has attracted extensive attention due to their potential applic...

    Authors: Hyemi Seo, Jisoo Hong, Taeyeol Kim, Won ll Choi, Daekyung Sung and Eunpyo Moon
    Citation: Journal of Biological Engineering 2019 13:94
  6. This paper introduces a new technique combining microdialysis with patch clamp to detect the changes in ATP (adenosine triphosphate) concentration in acupoints during acupuncture. The microdialysis probe was i...

    Authors: Yong Wu, Meng Huang, Ying Xia and Guanghong Ding
    Citation: Journal of Biological Engineering 2019 13:93
  7. Synthetic biology heavily depends on rapid and simple techniques for DNA engineering, such as Ligase Cycling Reaction (LCR), Gibson assembly and Golden Gate assembly, all of which allow for fast, multi-fragmen...

    Authors: Stefano Vecchione and Georg Fritz
    Citation: Journal of Biological Engineering 2019 13:92
  8. Transcription factor-based biosensors are useful tools for the detection of metabolites and industrially valuable molecules, and present many potential applications in biotechnology and biomedicine. However, t...

    Authors: Leopoldo F. M. Machado, Andrew Currin and Neil Dixon
    Citation: Journal of Biological Engineering 2019 13:91
  9. The production of transgenic chicken cells holds great promise for several diverse areas, including developmental biology and biomedical research. To this end, site-specific gene integration has been an attrac...

    Authors: Long Xie, Juanjuan Sun, Lifen Mo, Tianpeng Xu, Qaisar Shahzad, Dongyang Chen, Wenhao Yang, Yuying Liao and Yangqing Lu
    Citation: Journal of Biological Engineering 2019 13:90
  10. With the inherent high density and durable preservation, DNA has been recently recognized as a distinguished medium to store enormous data over millennia. To overcome the limitations existing in a recently rep...

    Authors: Yixin Wang, Md Noor-A-Rahim, Jingyun Zhang, Erry Gunawan, Yong Liang Guan and Chueh Loo Poh
    Citation: Journal of Biological Engineering 2019 13:89
  11. Exposure to air particulate matter (PM) is associated with various diseases in the human respiratory system. To date, most in vitro studies showing cellular responses to PM have been performed in cell culture ...

    Authors: Junhyoung Byun, Boa Song, Kyungwoo Lee, Byoungjae Kim, Hae Won Hwang, Myung-Ryul Ok, Hojeong Jeon, Kijeong Lee, Seung-Kuk Baek, Sang-Heon Kim, Seung Ja Oh and Tae Hoon Kim
    Citation: Journal of Biological Engineering 2019 13:88
  12. As an emerging research area of artificial enzymes, nanozyme, the catalytic nanomaterials with enzyme-like characteristics, have attracted enormous attention in research. Here, a nanozyme probe has been realiz...

    Authors: Aiyun Li, Lin Long, Fengshou Liu, Jianbo Liu, Xiaochun Wu and Yinglu Ji
    Citation: Journal of Biological Engineering 2019 13:87
  13. Large gap healing is a difficult issue in the recovery of peripheral nerve injury. The present study provides in vivo trials of silicone rubber chambers filled with collagen containing IFN-γ or IL-4 to bridge ...

    Authors: Chien-Fu Liao, Chung-Chia Chen, Yu-Wen Lu, Chun-Hsu Yao, Jia-Horng Lin, Tzong-Der Way, Tse-Yen Yang and Yueh-Sheng Chen
    Citation: Journal of Biological Engineering 2019 13:86
  14. Tissue engineering, as an interdisciplinary approach, is seeking to create tissues with optimal performance for clinical applications. Various factors, including cells, biomaterials, cell or tissue culture con...

    Authors: Azizeh Rahmani Del Bakhshayesh, Nahideh Asadi, Alireza Alihemmati, Hamid Tayefi Nasrabadi, Azadeh Montaseri, Soodabeh Davaran, Sepideh Saghati, Abolfazl Akbarzadeh and Ali Abedelahi
    Citation: Journal of Biological Engineering 2019 13:85
  15. The original version of this article [1] unfortunately included a typographical error to author Žiga Pušnik’s name.

    Authors: Žiga Pušnik, Miha Mraz, Nikolaj Zimic and Miha Moškon
    Citation: Journal of Biological Engineering 2019 13:84

    The original article was published in Journal of Biological Engineering 2019 13:75

  16. Attention has recently increased in the application of electrospun fibers because of their putative capability to create nanoscale platforms toward tissue engineering. To some extent, electrospun fibers are ap...

    Authors: Sonia Fathi Karkan, Soodabeh Davaran, Reza Rahbarghazi, Roya Salehi and Abolfazl Akbarzadeh
    Citation: Journal of Biological Engineering 2019 13:83
  17. The intestinal epithelium is a major site of drug metabolism in the human body, possessing enterocytes that house brush border enzymes and phase I and II drug metabolizing enzymes (DMEs). The enterocytes are s...

    Authors: Jennifer E. Speer, Yuli Wang, John K. Fallon, Philip C. Smith and Nancy L. Allbritton
    Citation: Journal of Biological Engineering 2019 13:82
  18. Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke. In the present study, a rec...

    Authors: Junyan Ma, Qian Li, Haidong Tan, Hao Jiang, Kuikui Li, Lihua Zhang, Quan Shi and Heng Yin
    Citation: Journal of Biological Engineering 2019 13:81
  19. The main issue in cutaneous regeneration is to develop engineered scaffolds based on natural extracellular matrix to promote dynamics of skin progenitor cells and accelerate differentiation into mature keratin...

    Authors: Leila Mohammadzadeh, Reza Rahbarghazi, Roya Salehi and Mehrdad Mahkam
    Citation: Journal of Biological Engineering 2019 13:79
  20. Influenza viruses cause hundreds of thousands of respiratory diseases worldwide each year, and vaccination is considered the most effective approach for preventing influenza annual epidemics or pandemics. Sinc...

    Authors: Chia-Chun Lai, Yu-Chieh Cheng, Pin-Wen Chen, Ting-Hui Lin, Tsai-Teng Tzeng, Chia-Chun Lu, Min-Shi Lee and Alan Yung-Chih Hu
    Citation: Journal of Biological Engineering 2019 13:78
  21. The baculovirus (BV) Autographa californica multiple nuclear polyhedrosis virus has been used in numerous protein expression systems because of its ability to infect insect cells and serves as a useful vaccinatio...

    Authors: Keigo Kondou, Tomoyuki Suzuki, Myint Oo Chang and Hiroshi Takaku
    Citation: Journal of Biological Engineering 2019 13:77
  22. The promise of biopharmaceuticals comprising one or more binding domains motivates the development of novel methods for de novo isolation and affinity maturation of virion-binding domains. Identifying avenues ...

    Authors: Pete Heinzelman, Alyssa Low, Rudo Simeon, Gus A. Wright and Zhilei Chen
    Citation: Journal of Biological Engineering 2019 13:76
  23. Gene regulatory networks with different topological and/or dynamical properties might exhibit similar behavior. System that is less perceptive for the perturbations of its internal and external factors should ...

    Authors: Žiga Pušnik, Miha Mraz, Nikolaj Zimic and Miha Moškon
    Citation: Journal of Biological Engineering 2019 13:75

    The Correction to this article has been published in Journal of Biological Engineering 2019 13:84

  24. Since their inception, human induced pluripotent stem cells (hiPSCs) have held much promise for pharmacological applications and cell-based therapies. However, their potential can only be realised if large num...

    Authors: Diogo E. S. Nogueira, Carlos A. V. Rodrigues, Marta S. Carvalho, Cláudia C. Miranda, Yas Hashimura, Sunghoon Jung, Brian Lee and Joaquim M. S. Cabral
    Citation: Journal of Biological Engineering 2019 13:74
  25. Jamming state transition has been used in literature to describe migrating-to-resting cell state transition during collective cell migration without proper rheological confirmation. Yield stress often has been...

    Authors: Ivana Pajic-Lijakovic and Milan Milivojevic
    Citation: Journal of Biological Engineering 2019 13:73
  26. The green algae balls (Aegagropila linnaei), known as Marimo, are large spherical colonies of live photosynthetic filaments, formed by rolling water currents in freshwater lakes. Photosynthesis therein produces g...

    Authors: Neil Phillips, Thomas C. Draper, Richard Mayne and Andrew Adamatzky
    Citation: Journal of Biological Engineering 2019 13:72
  27. MicroRNAs (miRNAs) are implicated in the progression of ischemic stroke (IS) and bone marrow-derived mesenchymal stem cells (BMSCs)-derived exosomes play a role in IS therapy. Herein we hypothesized that the B...

    Authors: Yiming Deng, Duanduan Chen, Feng Gao, Hong Lv, Guojun Zhang, Xuan Sun, Lian Liu, Dapeng Mo, Ning Ma, Ligang Song, Xiaochuan Huo, Tianyi Yan, Jingbo Zhang and Zhongrong Miao
    Citation: Journal of Biological Engineering 2019 13:71

    The Correction to this article has been published in Journal of Biological Engineering 2022 16:8

  28. Whole cell biosensors provide a simple method for the detection of heavy metals. However, previous designs of them rely primarily on simulation of heavy metal resistance systems of bacteria.

    Authors: Mingzhang Guo, Ruoxi Du, Zixin Xie, Xiaoyun He, Kunlun Huang, Yunbo Luo and Wentao Xu
    Citation: Journal of Biological Engineering 2019 13:70
  29. Non-alcoholic fatty liver disease (NAFLD) begins as simple hepatic steatosis, but further progress to chronic liver diseases results in severe liver damage and hepatic failure. However, therapeutic options are...

    Authors: Jae-Sung Ryu, Minji Lee, Seon Ju Mun, Sin-Hyoung Hong, Ho-Joon Lee, Hyo-Suk Ahn, Kyung-Sook Chung, Gun-Hwa Kim and Myung Jin Son
    Citation: Journal of Biological Engineering 2019 13:69
  30. Mesenchymal stem cells (MSCs) show tremendous promise as a cell source for tissue engineering and regenerative medicine, and are understood to be mechanosensitive to external mechanical environments. In recent...

    Authors: Tasneem Bouzid, Eunju Kim, Brandon D. Riehl, Amir Monemian Esfahani, Jordan Rosenbohm, Ruiguo Yang, Bin Duan and Jung Yul Lim
    Citation: Journal of Biological Engineering 2019 13:68
  31. Neuromodulation of central and peripheral neural circuitry brings together neurobiologists and neural engineers to develop advanced neural interfaces to decode and recapitulate the information encoded in the n...

    Authors: Benjamin J. Seicol, Sebastian Bejarano, Nicholas Behnke and Liang Guo
    Citation: Journal of Biological Engineering 2019 13:67
  32. Endophytic microorganisms are a rich source of bioactive natural products. They are considered as promising biofertilizers and biocontrol agents due to their growth-promoting interactions with the host plants ...

    Authors: Ozkan Fidan and Jixun Zhan
    Citation: Journal of Biological Engineering 2019 13:66
  33. Creating designer molecules using a combination of select domains from polyketide synthases and/or nonribosomal peptide synthetases (NRPS) continues to be a synthetic goal. However, an incomplete understanding...

    Authors: Fuchao Xu, Russell Butler, Kyle May, Megi Rexhepaj, Dayu Yu, Jiachen Zi, Yi Chen, Yonghong Liang, Jia Zeng, Joan Hevel and Jixun Zhan
    Citation: Journal of Biological Engineering 2019 13:65
  34. Significant rise in concentration of saline wastewater entering the treatment plants has been resulting in many problems in the biological treatment processes. On the other hand, the specific conditions of phy...

    Authors: Mostafa Tizghadam Ghazani and Alireza Taghdisian
    Citation: Journal of Biological Engineering 2019 13:64
  35. Silk glands are used by silkworms to spin silk fibers for making their cocoons. These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and ...

    Authors: Sheng Xu, Feng Wang, Yuancheng Wang, Riyuan Wang, Kai Hou, Chi Tian, Yanting Ji, Qianqian Yang, Ping Zhao and Qingyou Xia
    Citation: Journal of Biological Engineering 2019 13:61
  36. As a type of chronic autoimmune joint disease, rheumatoid arthritis (RA) is a disorder, characterized by a variety of physical symptoms as well as RA fibroblast-like synoviocyte (RA-FLS) proliferation. More re...

    Authors: Chun-Wang Zhang, Xia Wu, Dan Liu, Wei Zhou, Wei Tan, Yu-Xuan Fang, Yu Zhang, Yan-Qing Liu and Guo-Qing Li
    Citation: Journal of Biological Engineering 2019 13:60
  37. The production of recombinant proteins in mammalian cell lines is one of the most important areas in biopharmaceutical industry. Viral transcriptional promoters are widely used to express recombinant proteins ...

    Authors: Roberto A Zúñiga, Matías Gutiérrez-González, Norberto Collazo, Pablo Hérnan Sotelo, Carolina H Ribeiro, Claudia Altamirano, Carmen Lorenzo, Juan Carlos Aguillón and María Carmen Molina
    Citation: Journal of Biological Engineering 2019 13:59
  38. Traditional cancer therapies, such as surgery treatment, radiotherapy, and chemotherapy, often fail to completely eliminate tumor cells in an anaerobic microenvironment of tumor regions.

    Authors: Lian He, Huijun Yang, Jianli Tang, Zhudong Liu, Yiyan Chen, Binghua Lu, Haocheng He, Sijia Tang, Yunjun Sun, Fei Liu, Xuezhi Ding, Youming Zhang, Shengbiao Hu and Liqiu Xia
    Citation: Journal of Biological Engineering 2019 13:58
  39. The purpose of this review is to assess the state-of-the-art fabrication methods, advances in genome editing, and the use of machine learning to shape the prospective growth in cardiac tissue engineering. Thos...

    Authors: Anh H. Nguyen, Paul Marsh, Lauren Schmiess-Heine, Peter J. Burke, Abraham Lee, Juhyun Lee and Hung Cao
    Citation: Journal of Biological Engineering 2019 13:57
  40. Immunotoxins consisting of a toxin from bacteria or plants and a targeting module have been developed as potent anti-cancer therapeutics. The majority of them, especially those in preclinical or clinical testi...

    Authors: Byeong Sung Lee, Yumi Lee, Jisoo Park, Bo Seok Jeong, Migyeong Jo, Sang Taek Jung and Tae Hyeon Yoo
    Citation: Journal of Biological Engineering 2019 13:56
  41. Engineered living materials (ELMs) are an exciting new frontier, where living organisms create highly functional materials. In particular, protein ELMs have the advantage that their properties can be manipulat...

    Authors: Daniel T. Peters, Helen Waller, Mark A. Birch and Jeremy H. Lakey
    Citation: Journal of Biological Engineering 2019 13:54
  42. A transcriptional reporter is the key component in bacterial biosensors which are employed to monitor the induction or repression of a reporter gene corresponding to environmental change. Interaction of a tran...

    Authors: Xingjuan Chen, Xin Jiang, Cuijuan Tie, Jinnon Yoo, Yan Wang, Meiying Xu, Guoping Sun, Jun Guo and Xianqiang Li
    Citation: Journal of Biological Engineering 2019 13:53
  43. Biofilters are soil-plant based passive stormwater treatment systems which demonstrate promising, although inconsistent, removal of faecal microorganisms. Antimicrobial-producing plants represent a safe, inexp...

    Authors: P. Galbraith, R. Henry and D. T. McCarthy
    Citation: Journal of Biological Engineering 2019 13:52
  44. We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted b...

    Authors: Jun Ki Ahn, Hyo Yong Kim, Chang Yeol Lee, Ki Soo Park and Hyun Gyu Park
    Citation: Journal of Biological Engineering 2019 13:51