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  1. Biological systems have complicated environmental conditions that vary both spatially and temporally. It becomes necessary to impose time-varying soluble factor concentrations to study such systems, including ...

    Authors: Joshua Garrison, Zidong Li, Barath Palanisamy, Ling Wang and Erkin Seker

    Citation: Journal of Biological Engineering 2018 12:31

    Content type: Research

    Published on:

  2. Silkworm genetic engineering is widely used in gene function, silk engineering and disease-resistant engineering in most of Asia. Some of the earliest promoter elements are used to control the development of s...

    Authors: Zhan-Qi Dong, Zhi-Gang Hu, Hai-Qing Li, Ya-Ming Jiang, Ming-Ya Cao, Peng Chen, Cheng Lu and Min-Hui Pan

    Citation: Journal of Biological Engineering 2018 12:30

    Content type: Research

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  3. The retinal pigment epithelium (RPE) is essential to the health of the retina and the proper functioning of the photoreceptors. The RPE is rich in melanosomes, which contain the pigment melanin. Changes in RPE...

    Authors: Maryse Lapierre-Landry, Joseph Carroll and Melissa C. Skala

    Citation: Journal of Biological Engineering 2018 12:29

    Content type: Review

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  4. Cell-based therapy has expanded its influence in cancer immunotherapy, regenerative medicine, and tissue engineering. Due to their secretory functions, differentiation capabilities, specific homing effects thr...

    Authors: Daniel Y. Lee, Byung-Hyun Cha, Minjin Jung, Angela S. Kim, David A. Bull and Young-Wook Won

    Citation: Journal of Biological Engineering 2018 12:28

    Content type: Review

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  5. Standard microorganism isolating technology applied for complex multiphase environmental samples such as soil or sediment needs pre-treatment steps to remove living cells from their mixed-phase microniche, by ...

    Authors: M. V. Gorlenko, E. A. Chutko, E. S. Churbanova, N. V. Minaev, K. I. Kachesov, L. V. Lysak, S. A. Evlashin, V. S. Cheptsov, A. O. Rybaltovskiy, V. I. Yusupov, V. S. Zhigarkov, G. A. Davydova, B. N. Chichkov and V. N. Bagratashvili

    Citation: Journal of Biological Engineering 2018 12:27

    Content type: Research

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  6. In vitro expansion of mesenchymal stem cells (MSCs) has been implicated in loss of multipotency, leading to impaired chondrogenic potential and an eventual therapeutic effect, as reported in our previous study...

    Authors: Chong Shen, Tongmeng Jiang, Bo Zhu, Yiguan Le, Jianwei Liu, Zainen Qin, Haimin Chen, Gang Zhong, Li Zheng, Jinmin Zhao and Xingdong Zhang

    Citation: Journal of Biological Engineering 2018 12:26

    Content type: Research

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  7. Oleaginous fungi are efficient tools to convert agricultural waste streams into valuable components. The filamentous fungus Mucor circinelloides was cultivated in whey permeate, a byproduct from cheese production...

    Authors: Lauryn G. Chan, Joshua L. Cohen, Gulustan Ozturk, Marie Hennebelle, Ameer Y. Taha and Juliana Maria L. N. de Moura Bell

    Citation: Journal of Biological Engineering 2018 12:25

    Content type: Research

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  8. The seeding of scaffolds with the stromal vascular fraction (SVF) of adipose tissue is a common prevascularization strategy in tissue engineering. Alternatively, adipose tissue-derived microvascular fragments ...

    Authors: Thomas Später, Florian S. Frueh, Ruth M. Nickels, Michael D. Menger and Matthias W. Laschke

    Citation: Journal of Biological Engineering 2018 12:24

    Content type: Research

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  9. A primary objective of synthetic biology is the construction of genetic circuits with behaviors that can be predicted based on the properties of the constituent genetic parts from which they are built. However...

    Authors: Kalen P. Clifton, Ethan M. Jones, Sudip Paudel, John P. Marken, Callan E. Monette, Andrew D. Halleran, Lidia Epp and Margaret S. Saha

    Citation: Journal of Biological Engineering 2018 12:23

    Content type: Letters to the Editor

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  10. Fermentation condition optimization and nutrients screening are of equal importance for efficient production of plasmid DNA vaccines. This directly affects the downstream purification and final quality and yie...

    Authors: Juan Long, Xiao Zhao, Fei Liang, Nan Liu, Yuying Sun and Yongzhi Xi

    Citation: Journal of Biological Engineering 2018 12:22

    Content type: Research

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  11. The ability to direct the cellular response by means of biomaterial surface topography is important for biomedical applications. Substrate surface topography has been shown to be an effective cue for the regul...

    Authors: P. Formentín, Ú. Catalán, L. Pol, S. Fernández-Castillejo, R. Solà and L. F. Marsal

    Citation: Journal of Biological Engineering 2018 12:21

    Content type: Research

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  12. Human connective tissues are complex physiological microenvironments favorable for optimal survival, function, growth, proliferation, differentiation, migration, and death of tissue cells. Mimicking native tis...

    Authors: Girdhari Rijal and Weimin Li

    Citation: Journal of Biological Engineering 2018 12:20

    Content type: Review

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  13. Flow cytometry, with its high throughput nature, combined with the ability to measure an increasing number of cell parameters at once can surpass the throughput of prevalent genomic and metagenomic approaches ...

    Authors: Abhishek S. Dhoble, Pratik Lahiri and Kaustubh D. Bhalerao

    Citation: Journal of Biological Engineering 2018 12:19

    Content type: Methodology

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  14. Epithelia and endothelia delineate tissue compartments and control their environments by regulating the passage of ions and solutes. This barrier function is essential for the development and maintenance of mu...

    Authors: Jose Yeste, Xavi Illa, Mar Alvarez and Rosa Villa

    Citation: Journal of Biological Engineering 2018 12:18

    Content type: Review

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  15. Generation of reactive oxygen species (ROS), triggered by ultraviolet radiation (UVR), is associated with carcinogenesis of the skin. UV irradiation induced superoxide anion (O2•−) is the key ROS involved in the ...

    Authors: Li Xia Gao, Chunxiang Bian, Yan Wu, Muhammad Farrukh Nisar, Shida Chen, Chang Ming Li, Ling Yu, Ping Ji, Enyi Huang and Julia Li Zhong

    Citation: Journal of Biological Engineering 2018 12:17

    Content type: Methodology

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  16. Antibiotics, which are the most important medication in human history, have brought global concerns due to their potential risk to human health and environment by accelerating the development of drug-resistant...

    Authors: Sang Hoon Kim, Ee Taek Hwang and Man Bock Gu

    Citation: Journal of Biological Engineering 2018 12:16

    Content type: Research

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  17. Effects of shear stress on endothelium are important for the normal physiology of blood vessels and are implicated in the pathogenesis of atherosclerosis. They have been extensively studied in vitro. In one pa...

    Authors: Mean Ghim, Kuin T. Pang, Mehwish Arshad, Xiaomeng Wang and Peter D. Weinberg

    Citation: Journal of Biological Engineering 2018 12:15

    Content type: Methodology

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  18. Synthesis of a variety of biological circuits for specific functional purposes has made a tremendous progress in recent years. The ultimate goal of combining molecular biology and engineering is to realize a f...

    Authors: Chun-Liang Lin, Ting-Yu Kuo and Wei-Xian Li

    Citation: Journal of Biological Engineering 2018 12:14

    Content type: Research

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  19. Biosafety is a key aspect in the international Genetically Engineered Machine (iGEM) competition, which offers student teams an amazing opportunity to pursue their own research projects in the field of Synthet...

    Authors: Christopher M. Whitford, Saskia Dymek, Denise Kerkhoff, Camilla März, Olga Schmidt, Maximilian Edich, Julian Droste, Boas Pucker, Christian Rückert and Jörn Kalinowski

    Citation: Journal of Biological Engineering 2018 12:13

    Content type: Review

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  20. While hypoxia has been well-studied in various tumor microenvironments, its role in cancer cell dormancy is poorly understood, in part due to a lack of well-established in vitro and in vivo models. Hypoxic con...

    Authors: Hak Rae Lee, Faith Leslie and Samira M. Azarin

    Citation: Journal of Biological Engineering 2018 12:12

    Content type: Research

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  21. Anti-CRISPR proteins are expressed by phages as a reaction to the bacterial CRISPR–Cas defense system. Recently, the structures of anti-CRISPR proteins have been determined, and their diverse functions have be...

    Authors: Jing Li, Zengliang Xu, Aleksandr Chupalov and Mario Andrea Marchisio

    Citation: Journal of Biological Engineering 2018 12:11

    Content type: Research

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  22. Petrochemical wastewaters are highly polluting due to having various destructive materials such as aromatic hydrocarbons and heavy metal ions. Therefore, they need to be treated before disposal to the environm...

    Authors: Mahmood Derakhshan and Mojtaba Fazeli

    Citation: Journal of Biological Engineering 2018 12:10

    Content type: Research

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  23. Regulatory genes play critical roles in natural product biosynthetic pathways. Chromomycins are promising anticancer natural products from actinomycetes. This study is aimed to create an efficient strain for p...

    Authors: Lei Sun, Jia Zeng, Peiwu Cui, Wei Wang, Dayu Yu and Jixun Zhan

    Citation: Journal of Biological Engineering 2018 12:9

    Content type: Research

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  24. Coral reefs are colored by eukaryotic chromoproteins (CPs) that are homologous to green fluorescent protein. CPs differ from fluorescent proteins (FPs) by intensely absorbing visible light to give strong color...

    Authors: Josefine Liljeruhm, Saskia K. Funk, Sandra Tietscher, Anders D. Edlund, Sabri Jamal, Pikkei Wistrand-Yuen, Karl Dyrhage, Arvid Gynnå, Katarina Ivermark, Jessica Lövgren, Viktor Törnblom, Anders Virtanen, Erik R. Lundin, Erik Wistrand-Yuen and Anthony C. Forster

    Citation: Journal of Biological Engineering 2018 12:8

    Content type: Methodology

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  25. Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase...

    Authors: Fouzia Tabssum, Muhammad Irfan, Hafiz Abdullah Shakir and Javed Iqbal Qazi

    Citation: Journal of Biological Engineering 2018 12:7

    Content type: Research

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  26. Regenerative medicine and tissue engineering are promising approaches for organ transplantation. Extracellular matrix (ECM) based scaffolds obtained through the decellularization of natural organs have become ...

    Authors: Huajun Yu, Yunzhi Chen, Hongru Kong, Qikuan He, Hongwei Sun, Pravin Avinash Bhugul, Qiyu Zhang, Bicheng Chen and Mengtao Zhou

    Citation: Journal of Biological Engineering 2018 12:6

    Content type: Research

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  27. Islet transplantation is increasingly used in the diabetic patients to control the blood glucose level. However, the functional output of transplanted islets remains hampered due to the local inflammation, los...

    Authors: Kelei Zhu, Leqi Dong, Jinbo Wang, Dingyao Li, Mingliang Chen, Cunbin Jiang and Jinfa Wang

    Citation: Journal of Biological Engineering 2018 12:5

    Content type: Research

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  28. Protecting drinking water supplies from pathogens such as Cryptosporidium parvum is a major concern for water utilities worldwide. The sensitivity and specificity of current detection methods are largely determin...

    Authors: L. Diéguez, M. Winter, S. Molan, P. Monis, B. King and B. Thierry

    Citation: Journal of Biological Engineering 2018 12:4

    Content type: Research

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  29. Tryptophan derivatives such as 5-hydroxytryptophan (5HTP) and serotonin are valuable molecules with pharmaceutical interest. 5HTP is presently mainly obtained by extraction from the plant Griffonia simplicifolia ...

    Authors: José-Aníbal Mora-Villalobos and An-Ping Zeng

    Citation: Journal of Biological Engineering 2018 12:3

    Content type: Research

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  30. As a family of hormones with pleiotropic effects, natriuretic peptide (NP) system includes atrial NP (ANP), B-type NP (BNP), C-type NP (CNP), dendroaspis NP and urodilatin, with NP receptor-A (guanylate cyclas...

    Authors: Shihui Fu, Ping Ping, Fengqi Wang and Leiming Luo

    Citation: Journal of Biological Engineering 2018 12:2

    Content type: Review

    Published on:

  31. Viral infections often pose tremendous public health concerns as well as economic burdens. Despite the availability of vaccines or antiviral drugs, personal hygiene is considered as effective means as the firs...

    Authors: Yun Ha Lee, Yo Han Jang, Young-Seok Kim, Jinku Kim and Baik Lin Seong

    Citation: Journal of Biological Engineering 2018 12:1

    Content type: Letters to the Editor

    Published on:

  32. The study of simplified, ad-hoc constructed model systems can help to elucidate if quantitatively characterized biological parts can be effectively re-used in composite circuits to yield predictable functions....

    Authors: Lorenzo Pasotti, Massimo Bellato, Michela Casanova, Susanna Zucca, Maria Gabriella Cusella De Angelis and Paolo Magni

    Citation: Journal of Biological Engineering 2017 11:50

    Content type: Research

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  33. The repair or replacement of damaged skins is still an important, challenging public health problem. Immune acceptance and long-term survival of skin grafts represent the major problem to overcome in grafting ...

    Authors: Saurabh Dixit, Dieudonné R. Baganizi, Rajnish Sahu, Ejowke Dosunmu, Atul Chaudhari, Komal Vig, Shreekumar R. Pillai, Shree R. Singh and Vida A. Dennis

    Citation: Journal of Biological Engineering 2017 11:49

    Content type: Review

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  34. Needle-free, painless and localized drug delivery has been a coveted technology in the area of biomedical research. We present an innovative way of trans-dermal vaccine delivery using a miniature detonation-dr...

    Authors: Janardhanraj Subburaj, Akshay Datey, Jagadeesh Gopalan and Dipshikha Chakravortty

    Citation: Journal of Biological Engineering 2017 11:48

    Content type: Research

    Published on:

  35. The blood-brain barrier (BBB) is the interface between the vasculature and the brain, regulating molecular and cellular transport into the brain. Endothelial cells (ECs) that form the capillary walls constitut...

    Authors: John J. Jamieson, Peter C. Searson and Sharon Gerecht

    Citation: Journal of Biological Engineering 2017 11:37

    Content type: Review

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  37. In the past two decades, methods have been developed to measure the mechanical properties of single biomolecules. One of these methods, Magnetic tweezers, is amenable to acquisition of data on many single mole...

    Authors: Keith C. Johnson, Emilie Clemmens, Hani Mahmoud, Robin Kirkpatrick, Juan C. Vizcarra and Wendy E. Thomas

    Citation: Journal of Biological Engineering 2017 11:47

    Content type: Methodology

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  38. Liver disease contributes significantly to global disease burden and is associated with rising incidence and escalating costs. It is likely that innovative approaches, arising from the emerging field of liver ...

    Authors: Ogechi Ogoke, Janet Oluwole and Natesh Parashurama

    Citation: Journal of Biological Engineering 2017 11:46

    Content type: Review

    Published on:

  39. Targeted nucleases have transformed genome editing technology, providing more efficient methods to make targeted changes in mammalian genome. In parallel, there is an increasing demand of Cre-LoxP technology f...

    Authors: Ranjita Sengupta, Amy Mendenhall, Nandita Sarkar, Chandreyee Mukherjee, Amirali Afshari, Joseph Huang and Biao Lu

    Citation: Journal of Biological Engineering 2017 11:45

    Content type: Methodology

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  40. Electronic lab notebooks (ELNs) are better equipped than paper lab notebooks (PLNs) to handle present-day life science and engineering experiments that generate large data sets and require high levels of data ...

    Authors: Erin M. Riley, Holly Z. Hattaway and P. Arthur Felse

    Citation: Journal of Biological Engineering 2017 11:40

    Content type: Research

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  41. Previously we demonstrated that an entire bacterial operon (the PRN operon) is expressible in plants when driven by the Tomato -yellow-leaf-curl-virus (TYLCV) -derived universal vector IL-60.

    Authors: Rita Mozes-Koch, Edna Tanne, Alexandra Brodezki, Ran Yehuda, Ofer Gover, Haim D. Rabinowitch and Ilan Sela

    Citation: Journal of Biological Engineering 2017 11:44

    Content type: Research

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  42. This study examines the effects of adding gelatin to a starch-chitosan composite foam, focusing on the altered structural and biological properties. The compressive modulus of foams containing different gelati...

    Authors: Gregory E. Risser, Brittany L. Banik, Justin L. Brown and Jeffrey M. Catchmark

    Citation: Journal of Biological Engineering 2017 11:43

    Content type: Research

    Published on:

  43. Locomotor activity of rodents is an important readout to assess well-being and physical health, and is pivotal for behavioral phenotyping. Measuring homecage-activity with standard and cost-effective optical m...

    Authors: Andreas Genewsky, Daniel E. Heinz, Paul M. Kaplick, Kasyoka Kilonzo and Carsten T. Wotjak

    Citation: Journal of Biological Engineering 2017 11:36

    Content type: Methodology

    Published on:

  44. The accumulated evidence points to the microenvironment as the primary mediator of cellular fate determination. Comprised of parenchymal cells, stromal cells, structural extracellular matrix proteins, and sign...

    Authors: Patrick C. Sachs, Peter A. Mollica and Robert D. Bruno

    Citation: Journal of Biological Engineering 2017 11:34

    Content type: Review

    Published on:

  45. Oxygen-responsive promoters can be useful for synthetic biology applications, however, information on their characteristics is still limited. Here, we characterized a group of heterologous microaerobic globin ...

    Authors: Alvaro R. Lara, Karim E. Jaén, Juan-Carlos Sigala, Lars Regestein and Jochen Büchs

    Citation: Journal of Biological Engineering 2017 11:39

    Content type: Letters to the Editor

    Published on:

  46. Aerobic granular sludge has become an attractive alternative to the conventional activated sludge due to its high settling velocity, compact structure, and higher tolerance to toxic substances and adverse cond...

    Authors: Yanan Ren, Fernanda Ferraz, Abbass Jafari Kang and Qiuyan Yuan

    Citation: Journal of Biological Engineering 2017 11:42

    Content type: Research

    Published on:

  47. This article explains some simple experiments that can be used in undergraduate or graduate physics or biomedical engineering laboratory classes to learn how birdcage volume radiofrequency (RF) coils and magne...

    Authors: Dwight E. Vincent, Tianhao Wang, Thalia A. K. Magyar, Peni I. Jacob, Richard Buist and Melanie Martin

    Citation: Journal of Biological Engineering 2017 11:41

    Content type: Research

    Published on:

  48. UP elements (upstream element) are DNA sequences upstream of a promoter that interact with the α-subunit of RNA polymerase (RNAP) and can affect transcription by altering the binding RNAP to DNA. However, deta...

    Authors: Qiang Yan and Stephen S. Fong

    Citation: Journal of Biological Engineering 2017 11:33

    Content type: Research

    Published on:

  49. Human induced pluripotent stem cells (hiPSCs) can form any tissue found in the body, making them attractive for regenerative medicine applications. Seeding hiPSC aggregates into biomaterial scaffolds can contr...

    Authors: Meghan E. Hall, Nima Khadem Mohtaram, Stephanie M. Willerth and Roderick Edwards

    Citation: Journal of Biological Engineering 2017 11:38

    Content type: Research

    Published on:

  50. The primary objective of Tissue engineering is a regeneration or replacement of tissues or organs damaged by disease, injury, or congenital anomalies. At present, Tissue engineering repairs damaged tissues and...

    Authors: Aleš Gregor, Eva Filová, Martin Novák, Jakub Kronek, Hynek Chlup, Matěj Buzgo, Veronika Blahnová, Věra Lukášová, Martin Bartoš, Alois Nečas and Jan Hošek

    Citation: Journal of Biological Engineering 2017 11:31

    Content type: Research

    Published on:

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  • ISSN: 1754-1611 (electronic)

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Journal of Biological Engineering

ISSN: 1754-1611