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  1. Content type: Methodology

    Escherichia coli is often used for recombinant protein production. The expression of recombinant proteins negatively affects the microbial growth, thus, a balance between protein expre...

    Authors: Tobias Ladner, Martina Mühlmann, Andreas Schulte, Georg Wandrey and Jochen Büchs

    Citation: Journal of Biological Engineering 2017 11:20

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  2. Content type: Research

    Synechococcus sp. PCC 7002 (henceforth Synechococcus) is developing into a powerful synthetic biology chassis. In order to streamline the integration of genes into the Synechococcus ch...

    Authors: Anne Ilse Maria Vogel, Rahmi Lale and Martin Frank Hohmann-Marriott

    Citation: Journal of Biological Engineering 2017 11:19

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  3. Content type: Research

    High-throughput methods based on molecular reporters have greatly advanced our knowledge of cell signaling in mammalian cells. However, their ability to monitor various types of cells is markedly limited by th...

    Authors: Zhiwen Zhang, Zachary Stickney, Natalie Duong, Kevin Curley and Biao Lu

    Citation: Journal of Biological Engineering 2017 11:18

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  4. Content type: Research

    Probiotic bacteria are becoming an important tool for improving human health, controlling diseases and enhancing immune responses. The availability of a cost effective cultivation conditions has profound effec...

    Authors: Asma Manzoor, Javed Iqbal Qazi, Ikram ul Haq, Hamid Mukhtar and Akhtar Rasool

    Citation: Journal of Biological Engineering 2017 11:17

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  5. Content type: Review

    Two important and large non-point sources of nitrogen in residential areas that adversely affect water quality are stormwater runoff and effluent from on-site treatment systems. These sources are challenging t...

    Authors: E. V. Lopez-Ponnada, T. J. Lynn, M. Peterson, S. J. Ergas and J. R. Mihelcic

    Citation: Journal of Biological Engineering 2017 11:16

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  6. Content type: Research

    Umbelliferone, also known as 7-hydroxycoumarin, is a phenolic metabolite found in many familiar plants. Its derivatives have been shown to have various pharmacological and chemo-preventive effects on human hea...

    Authors: Luan Luong Chu, Ramesh Prasad Pandey, Haet Nim Lim, Hye Jin Jung, Nguyen Huy Thuan, Tae-Su Kim and Jae Kyung Sohng

    Citation: Journal of Biological Engineering 2017 11:15

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  7. Content type: Research

    Physical scaffolds are useful for supporting cells to form three-dimensional (3D) tissue. However, it is non-trivial to develop a scheme that can robustly guide cells to self-organize into a tissue with the de...

    Authors: Xiaolu Zhu, Shiva Gojgini, Ting-Hsuan Chen, Peng Fei, Siyan Dong, Chih-Ming Ho and Tatiana Segura

    Citation: Journal of Biological Engineering 2017 11:12

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  8. Content type: Research

    Nuclear magnetic resonance (NMR) technique is a powerful analytical tool in determining the presence of bacterial contaminants in complex biological samples. In this paper, a portable NMR-based (pNMR) biosenso...

    Authors: Yilun Luo and Evangelyn C. Alocilja

    Citation: Journal of Biological Engineering 2017 11:14

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  9. Content type: Review

    Nanomedicine has seen a significant increase in research on stimuli-responsive activatable nanoprobes for tumor-specific delivery and diagnosis. The tumor microenvironment has particular characteristics that c...

    Authors: Byunghoon Kang, Aastha Kukreja, Daesub Song, Yong-Min Huh and Seungjoo Haam

    Citation: Journal of Biological Engineering 2017 11:13

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  10. Content type: Review

    Sandwich-type biosensor platforms have drawn lots of attentions due to its superior features, compared to other platforms, in terms of its stable and reproducible responses and easy enhancement in the detectio...

    Authors: Ho Bin Seo and Man Bock Gu

    Citation: Journal of Biological Engineering 2017 11:11

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  11. Content type: Methodology

    Quantifying gene expression at single cell level is fundamental for the complete characterization of synthetic gene circuits, due to the significant impact of noise and inter-cellular variability on the system...

    Authors: Marilisa Cortesi, Lucia Bandiera, Alice Pasini, Alessandro Bevilacqua, Alessandro Gherardi, Simone Furini and Emanuele Giordano

    Citation: Journal of Biological Engineering 2017 11:8

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  12. Content type: Research

    It is challenging to achieve ultrasensitive and selective detection of waterborne pathogens at extremely low levels (i.e., single cell/mL) using conventional methods. Even with molecular methods such as ELISA ...

    Authors: Chao Wang, Foram Madiyar, Chenxu Yu and Jun Li

    Citation: Journal of Biological Engineering 2017 11:9

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  13. Content type: Research

    Deposits of aggregated amyloid-β protein (Aβ) are a pathological hallmark of Alzheimer’s disease (AD). Thus, one therapeutic strategy is to eliminate these deposits by halting Aβ aggregation. While a variety o...

    Authors: Kelly A. Moore, Kayla M. Pate, Deborah D. Soto-Ortega, Samuel Lohse, Nicholas van der Munnik, Mihyun Lim, Kaliah S. Jackson, Venetia D. Lyles, Lemeisha Jones, Nisha Glassgow, Vanessa M. Napumecheno, Shanee Mobley, Mark J. Uline, Rahina Mahtab, Catherine J. Murphy and Melissa A. Moss

    Citation: Journal of Biological Engineering 2017 11:5

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  14. Content type: Research

    During hydrous ethanol production from the sugar refinery industry in Mexico, vinasse is generated. Phenolic compounds and melanoidins contribute to its color and make degradation of the vinasse a difficult ta...

    Authors: Elda España-Gamboa, Teresa Vicent, Xavier Font, Jorge Dominguez-Maldonado, Blondy Canto-Canché and Liliana Alzate-Gaviria

    Citation: Journal of Biological Engineering 2017 11:6

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  15. Content type: Research

    Microbial consortia composed of autotrophic and heterotrophic species abound in nature, yet examples of synthetic communities with mixed metabolism are limited in the laboratory. We previously engineered a mod...

    Authors: Stephanie G. Hays, Leo L. W. Yan, Pamela A. Silver and Daniel C. Ducat

    Citation: Journal of Biological Engineering 2017 11:4

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  16. Content type: Research

    A viable single cell is crucial for studies of single cell biology. In this paper, laser-induced forward transfer (LIFT) was used to isolate individual cell with a closed chamber designed to avoid contaminatio...

    Authors: Yu Deng, Philippe Renaud, Zhongning Guo, Zhigang Huang and Ying Chen

    Citation: Journal of Biological Engineering 2017 11:2

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  17. Content type: Research

    Cellulases are key player in the hydrolyzation of cellulose. Unfortunately, this reaction is slow and a bottleneck in the process chain from biomass to intermediates and biofuels due to low activities of the e...

    Authors: Martina Mühlmann, Martin Kunze, Joaquim Ribeiro, Bertram Geinitz, Christian Lehmann, Ulrich Schwaneberg, Ulrich Commandeur and Jochen Büchs

    Citation: Journal of Biological Engineering 2017 11:1

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  18. Content type: Research

    Biofilm-based microalgal growth was determined as functions of organic chemical loading and water temperature utilizing dairy wastewater from a full-scale dairy farm. The dairy industry is a significant source...

    Authors: Zachary T. Fica and Ronald C. Sims

    Citation: Journal of Biological Engineering 2016 10:18

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  19. Content type: Review

    There have been many trials to visualize smell using various techniques in order to objectively express the smell because information obtained from the sense of smell in human is very subjective. So far, well-...

    Authors: Hwi Jin Ko and Tai Hyun Park

    Citation: Journal of Biological Engineering 2016 10:17

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  20. Content type: Review

    Each cell forever interacts with its extracellular matrix (ECM); a stem cell relies on this interaction to guide differentiation. The stiffness, nanotopography, protein composition, stress and strain inherent ...

    Authors: William J. Hadden and Yu Suk Choi

    Citation: Journal of Biological Engineering 2016 10:16

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  21. Content type: Research

    Cycles of covalent modification are ubiquitous motifs in cellular signalling. Although such signalling cycles are implemented via a highly concise set of chemical reactions, they have been shown to be capable ...

    Authors: Mathias Foo, Rucha Sawlekar and Declan G. Bates

    Citation: Journal of Biological Engineering 2016 10:15

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  22. Content type: Research

    Small-scale micro-bioreactors have become the cultivation vessel of choice during the first steps of bioprocess development. They combine high cultivation throughput with enhanced cost efficiency per cultivati...

    Authors: David Flitsch, Sebastian Krabbe, Tobias Ladner, Mario Beckers, Jana Schilling, Stefan Mahr, Uwe Conrath, Werner K. Schomburg and Jochen Büchs

    Citation: Journal of Biological Engineering 2016 10:14

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  23. Content type: Research

    Alkaline amylase has significant potential for applications in the textile, paper and detergent industries, however, low yield of which cannot meet the requirement of industrial application. In this work, a no...

    Authors: Yingfang Ma, Wei Shen, Xianzhong Chen, Long Liu, Zhemin Zhou, Fei Xu and Haiquan Yang

    Citation: Journal of Biological Engineering 2016 10:13

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  24. Content type: Research

    We propose, model, and implement a novel system of population-level intervention against a virus. One context is a treatment against a chronic infection such as HIV. The underlying principle is a form of virus...

    Authors: Matthew L. Paff, Scott L. Nuismer, Andrew D. Ellington, Ian J. Molineux, Ryan H. May and James J. Bull

    Citation: Journal of Biological Engineering 2016 10:12

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  25. Content type: Research

    Genetic code expansion has developed into an elegant tool to incorporate unnatural amino acids (uAA) at predefined sites in the protein backbone in response to an amber codon. However, recombinant production a...

    Authors: Georg Wandrey, Joel Wurzel, Kyra Hoffmann, Tobias Ladner, Jochen Büchs, Lorenz Meinel and Tessa Lühmann

    Citation: Journal of Biological Engineering 2016 10:11

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  26. Content type: Research

    The rapidly evolving discipline of biological and biomedical engineering requires adaptive instructional approaches that teach students to target and solve multi-pronged and ill-structured problems at the cutt...

    Authors: Ritu Raman, Marlon Mitchell, Pablo Perez-Pinera, Rashid Bashir and Lizanne DeStefano

    Citation: Journal of Biological Engineering 2016 10:10

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  27. Content type: Research

    In this study, a hybrid treatment system (Fluidized Bed positioned in a biological reactor of an Activated Sludge process) was used to treat saline domestic wastewater. The performance of the mentioned hybrid ...

    Authors: N. Salmanikhas, M. Tizghadam and A. Rashidi Mehrabadi

    Citation: Journal of Biological Engineering 2016 10:9

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  28. Content type: Research

    During the downstream process of bio-based bulk chemicals, organic impurities, mostly residues from the fermentation process, must be separated to obtain a pure and ready-to-market chemical. In this study, cap...

    Authors: Hendrik Laube, Frank-Michael Matysik, Andreas Schmidberger, Kerstin Mehlmann, Andreas Toursel and Jana Boden

    Citation: Journal of Biological Engineering 2016 10:7

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  29. Content type: Letter to the Editor

    CRISPR assisted homology directed repair enables the introduction of virtually any modification to the Saccharomyces cerevisiae genome. Of obvious interest is the marker-free and seamless introduction of point mu...

    Authors: Damien Biot-Pelletier and Vincent J. J. Martin

    Citation: Journal of Biological Engineering 2016 10:6

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  30. Content type: Research

    In recent years, there have been intensive efforts to develop synthetic microbial platforms for the production, biosensing and bio-remediation of fossil fuel constituents such as alkanes. Building predictable ...

    Authors: Toby P. Call, M. Kalim Akhtar, Frank Baganz and Chris Grant

    Citation: Journal of Biological Engineering 2016 10:5

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  31. Content type: Research

    Synthetic biology aims to engineer biological systems for desired behaviors. The construction of these systems can be complex, often requiring genetic reprogramming, extensive de novo DNA synthesis, and functi...

    Authors: Gregory Linshiz, Erik Jensen, Nina Stawski, Changhao Bi, Nick Elsbree, Hong Jiao, Jungkyu Kim, Richard Mathies, Jay D. Keasling and Nathan J. Hillson

    Citation: Journal of Biological Engineering 2016 10:3

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  32. Content type: Research

    Surface-enhanced Raman scattering (SERS) is a powerful light scattering technique that can be used for sensitive immunoassay development and cell labeling. A major obstacle to using SERS is the complexity of f...

    Authors: Nathan D. Israelsen, Donald Wooley, Cynthia Hanson and Elizabeth Vargis

    Citation: Journal of Biological Engineering 2016 10:2

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  33. Content type: Erratum

    Authors: Afnan Azizi, Wilson Lam, Hilary Phenix, Lioudmila Tepliakova, Ian J. Roney, Daniel Jedrysiak, Alex Power, Vaibhav Gupta, Nada Elnour, Martin Hanzel, Alexandra C. Tzahristos, Shihab Sarwar and Mads Kærn

    Citation: Journal of Biological Engineering 2016 10:1

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    The original article was published in Journal of Biological Engineering 2015 9:8

  34. Content type: Research

    Plant terpenoids are known for their diversity, stereochemical complexity, and their commercial interest as pharmaceuticals, food additives, and cosmetics. Developing biotechnology approaches for the productio...

    Authors: Thiyagarajan Gnanasekaran, Konstantinos Vavitsas, Johan Andersen-Ranberg, Agnieszka Zygadlo Nielsen, Carl Erik Olsen, Björn Hamberger and Poul Erik Jensen

    Citation: Journal of Biological Engineering 2015 9:24

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  35. Content type: Research

    Imbalances in gene expression of a metabolic pathway can result in less-yield of the desired products. Several targets were intensively investigated to balance the gene expression, such as promoter, ribosome b...

    Authors: Shasha Zhang, Xuejin Zhao, Yong Tao and Chunbo Lou

    Citation: Journal of Biological Engineering 2015 9:23

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  36. Content type: Research

    In the field of biomedical engineering, many studies have focused on the possible applications of graphene and related nanomaterials due to their potential for use as scaffolds, coating materials and delivery ...

    Authors: Yong Cheol Shin, Jong Ho Lee, Min Jeong Kim, Suck Won Hong, Bongju Kim, Jung Keun Hyun, Yu Suk Choi, Jong-Chul Park and Dong-Wook Han

    Citation: Journal of Biological Engineering 2015 9:22

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  37. Content type: Research

    Cell migration is a vital process for growth and repair. In vitro migration assays, utilized to study cell migration, often rely on physical scraping of a cell monolayer to induce cell migration. The physical act...

    Authors: Kaitlyn R. Ammann, Katrina J. DeCook, Phat L. Tran, Valerie M. Merkle, Pak K. Wong and Marvin J. Slepian

    Citation: Journal of Biological Engineering 2015 9:19

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  38. Content type: Research

    The interaction of stem cells with their culture substrates is critical in controlling their fate and function. Declining stemness of adult-derived human mesenchymal stem cells (hMSCs) during in vitro expansion o...

    Authors: Lucas H. Hofmeister, Lino Costa, Daniel A. Balikov, Spencer W. Crowder, Alexander Terekhov, Hak-Joon Sung and William H. Hofmeister

    Citation: Journal of Biological Engineering 2015 9:18

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  39. Content type: Letters to the Editor

    There is a general assent on the key role of standards in Synthetic Biology. In two consecutive letters to this journal, suggestions on the assembly methods for the Registry of standard biological parts have b...

    Authors: Cristina Vilanova, Kristie Tanner, Pedro Dorado-Morales, Paula Villaescusa, Divya Chugani, Alba Frías, Ernesto Segredo, Xavier Molero, Marco Fritschi, Lucas Morales, Daniel Ramón, Carlos Peña, Juli Peretó and Manuel Porcar

    Citation: Journal of Biological Engineering 2015 9:17

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  40. Content type: Methodology

    Molecular cloning is an essential step in biological engineering. Methods involving megaprimer-based PCR of a whole plasmid are promising alternatives to the traditional restriction-ligation-based molecular cl...

    Authors: Pawel Jajesniak and Tuck Seng Wong

    Citation: Journal of Biological Engineering 2015 9:15

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